دورية أكاديمية
Identification of trypsin-degrading commensals in the large intestine.
| العنوان: | Identification of trypsin-degrading commensals in the large intestine. |
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| المؤلفون: | Li Y; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan.; Institute for Cancer Research, Faculty of Medicine, University of Oslo, Oslo, Norway., Watanabe E; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan.; Department of Pediatric Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan., Kawashima Y; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Japan., Plichta DR; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wang Z; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Ujike M; Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan., Ang QY; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wu R; Interdisciplinary Life Science-PULSe, Purdue University, West Lafayette, IN, USA., Furuichi M; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Takeshita K; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Yoshida K; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Nishiyama K; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Kearney SM; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Suda W; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan., Hattori M; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Cooperative Major in Advanced Health Science, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan., Sasajima S; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Matsunaga T; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan., Zhang X; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan., Watanabe K; JSR-Keio University Medical and Chemical Innovation Center, Keio University School of Medicine, Tokyo, Japan., Fujishiro J; Department of Pediatric Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan., Norman JM; Vedanta Biosciences, Cambridge, MA, USA., Olle B; Vedanta Biosciences, Cambridge, MA, USA., Matsuyama S; Department of Virology III, National Institute of Infectious Diseases, Tokyo, Japan., Namkoong H; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan., Uwamino Y; Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan., Ishii M; Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo, Japan., Fukunaga K; Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo, Japan., Hasegawa N; Department of Infectious Diseases, Keio University School of Medicine, Tokyo, Japan., Ohara O; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.; Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Japan., Xavier RJ; Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA. xavier@molbio.mgh.harvard.edu.; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA. xavier@molbio.mgh.harvard.edu.; Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. xavier@molbio.mgh.harvard.edu., Atarashi K; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan. kojiatarashi@keio.jp.; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan. kojiatarashi@keio.jp.; JSR-Keio University Medical and Chemical Innovation Center, Keio University School of Medicine, Tokyo, Japan. kojiatarashi@keio.jp., Honda K; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan. kenya@keio.jp.; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan. kenya@keio.jp.; JSR-Keio University Medical and Chemical Innovation Center, Keio University School of Medicine, Tokyo, Japan. kenya@keio.jp. |
| المصدر: | Nature [Nature] 2022 Sep; Vol. 609 (7927), pp. 582-589. Date of Electronic Publication: 2022 Sep 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basingstoke : Nature Publishing Group Original Publication: London, Macmillan Journals ltd. |
| مواضيع طبية MeSH: | Gastrointestinal Microbiome*/genetics , Intestine, Large*/metabolism , Intestine, Large*/microbiology , Symbiosis* , Trypsin*/metabolism, Administration, Oral ; Animals ; Bacterial Secretion Systems ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/immunology ; Bacteroidetes/isolation & purification ; Bacteroidetes/metabolism ; COVID-19/complications ; Citrobacter rodentium/immunology ; Diarrhea/complications ; Feces/microbiology ; Humans ; Immunoglobulin A/metabolism ; Mice ; Murine hepatitis virus/metabolism ; Murine hepatitis virus/pathogenicity ; Proteolysis ; SARS-CoV-2/pathogenicity ; Virus Internalization |
| مستخلص: | Increased levels of proteases, such as trypsin, in the distal intestine have been implicated in intestinal pathological conditions 1-3 . However, the players and mechanisms that underlie protease regulation in the intestinal lumen have remained unclear. Here we show that Paraprevotella strains isolated from the faecal microbiome of healthy human donors are potent trypsin-degrading commensals. Mechanistically, Paraprevotella recruit trypsin to the bacterial surface through type IX secretion system-dependent polysaccharide-anchoring proteins to promote trypsin autolysis. Paraprevotella colonization protects IgA from trypsin degradation and enhances the effectiveness of oral vaccines against Citrobacter rodentium. Moreover, Paraprevotella colonization inhibits lethal infection with murine hepatitis virus-2, a mouse coronavirus that is dependent on trypsin and trypsin-like proteases for entry into host cells 4,5 . Consistently, carriage of putative genes involved in trypsin degradation in the gut microbiome was associated with reduced severity of diarrhoea in patients with SARS-CoV-2 infection. Thus, trypsin-degrading commensal colonization may contribute to the maintenance of intestinal homeostasis and protection from pathogen infection. (© 2022. The Author(s).) |
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| معلومات مُعتمدة: | P30 DK043351 United States DK NIDDK NIH HHS; R01 AT009708 United States AT NCCIH NIH HHS |
| المشرفين على المادة: | 0 (Bacterial Secretion Systems) 0 (Bacterial Vaccines) 0 (Immunoglobulin A) EC 3.4.21.4 (Trypsin) |
| تواريخ الأحداث: | Date Created: 20220907 Date Completed: 20220919 Latest Revision: 20220930 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9477747 |
| DOI: | 10.1038/s41586-022-05181-3 |
| PMID: | 36071157 |
| قاعدة البيانات: | MEDLINE |
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| DOI: | 10.1038/s41586-022-05181-3 |