دورية أكاديمية
Development of anti-Crimean-Congo hemorrhagic fever virus Gc and NP-specific ELISA for detection of antibodies in domestic animal sera.
| العنوان: | Development of anti-Crimean-Congo hemorrhagic fever virus Gc and NP-specific ELISA for detection of antibodies in domestic animal sera. |
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| المؤلفون: | Belij-Rammerstorfer S; Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom., Limon G; The Pirbright Institute, Woking, United Kingdom., Maze EA; The Pirbright Institute, Woking, United Kingdom., Hannant K; The Pirbright Institute, Woking, United Kingdom., Hughes E; Medical Research Council (MRC)-University of Glasgow Centre for Virus Research (CRV), Glasgow, United Kingdom., Tchakarova SR; Bulgarian Food Safety Agency, Sofia, Bulgaria., Alexandrov T; Bulgarian Food Safety Agency, Sofia, Bulgaria., Mmbaga BT; Kilimanjaro Clinical Research Institute (KCRI), Kilimanjaro Christian Medical Centre, Moshi, Tanzania.; Kilimanjaro Christian Medical University College, Moshi, Tanzania., Willett B; Medical Research Council (MRC)-University of Glasgow Centre for Virus Research (CRV), Glasgow, United Kingdom., Booth G; The Pirbright Institute, Woking, United Kingdom., Lyons NA; The Pirbright Institute, Woking, United Kingdom., Baker N; Research and Evaluation, UK Health Security Agency, Porton Down, United Kingdom., Thomas KM; Research and Evaluation, UK Health Security Agency, Porton Down, United Kingdom., Wright D; Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom., Saunders J; Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom., Browning C; The Pirbright Institute, Woking, United Kingdom., Wilsden G; The Pirbright Institute, Woking, United Kingdom., Carroll M; Pandemic Science Institute, Wellcome Centre for Human Genetics, Nuffield Department of Medicine, Oxford University, Oxford, United Kingdom., Hewson R; Research and Evaluation, UK Health Security Agency, Porton Down, United Kingdom.; Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom., Charleston B; The Pirbright Institute, Woking, United Kingdom., Lambe T; Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom., Ludi AB; The Pirbright Institute, Woking, United Kingdom. |
| المصدر: | Frontiers in veterinary science [Front Vet Sci] 2022 Aug 25; Vol. 9, pp. 913046. Date of Electronic Publication: 2022 Aug 25 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Crimean-Congo hemorrhagic fever (CCHF) is a priority emerging disease. CCHF, caused by the CCHF virus (CCHFV), can lead to hemorrhagic fever in humans with severe cases often having fatal outcomes. CCHFV is maintained within a tick-vertebrate-tick cycle, which includes domestic animals. Domestic animals infected with CCHFV do not show clinical signs of the disease and the presence of antibodies in the serum can provide evidence of their exposure to the virus. Current serological tests are specific to either one CCHFV antigen or the whole virus antigen. Here, we present the development of two in-house ELISAs for the detection of serum IgG that is specific for two different CCHFV antigens: glycoprotein Gc (CCHFV Gc) and nucleoprotein (CCHFV NP). We demonstrate that these two assays were able to detect anti-CCHFV Gc-specific and anti-CCHFV NP-specific IgG in sheep from endemic CCHFV areas with high specificity, providing new insight into the heterogeneity of the immune response induced by natural infection with CCHFV in domestic animals. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Belij-Rammerstorfer, Limon, Maze, Hannant, Hughes, Tchakarova, Alexandrov, Mmbaga, Willett, Booth, Lyons, Baker, Thomas, Wright, Saunders, Browning, Wilsden, Carroll, Hewson, Charleston, Lambe and Ludi.) |
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| معلومات مُعتمدة: | BBS/E/I/00007036 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| فهرسة مساهمة: | Keywords: CCHFV Gc; CCHFV NP; Crimean-Congo hemorrhagic fever; ELISA; antibody response; domestic animals |
| تواريخ الأحداث: | Date Created: 20220912 Latest Revision: 20240228 |
| رمز التحديث: | 20240228 |
| مُعرف محوري في PubMed: | PMC9454098 |
| DOI: | 10.3389/fvets.2022.913046 |
| PMID: | 36090164 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2297-1769 |
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| DOI: | 10.3389/fvets.2022.913046 |