دورية أكاديمية
Bovine Lactoferrin Induces Cell Death in Human Prostate Cancer Cells.
| العنوان: | Bovine Lactoferrin Induces Cell Death in Human Prostate Cancer Cells. |
|---|---|
| المؤلفون: | Rocha VP; Department of Biochemistry, Biomedical Institute, UNIRIO, 20211-040, Brazil.; Cell and Molecular Biology Graduate Program, UNIRIO, 20211-040, Brazil., Campos SPC; Medical Biochemistry Institute, UFRJ, 21941-590, Brazil., Barros CA; Department of Biochemistry, Biomedical Institute, UNIRIO, 20211-040, Brazil.; Multicentre Graduate Program in Biochemistry and Molecular Biology, IFRJ, 20270-021, Brazil., Trindade P; Cell and Molecular Biology Graduate Program, UNIRIO, 20211-040, Brazil.; D'Or Institute for Research and Education-IDOR, 22281-100, Brazil., Souza LRQ; D'Or Institute for Research and Education-IDOR, 22281-100, Brazil., Silva TG; National Center for Structural Biology and Bioimaging, CENABIO, UFRJ, 21941-902, Brazil., Gimba ERP; Natural Science Department, UFF, 28895-532, Brazil.; Cellular and Molecular Oncology Program, National Institute of Cancer (INCA), 20231-050, Brazil., Teodoro AJ; Department of Nutrition and Dietetics, UFF, 22290-250, Brazil., Gonçalves RB; Department of Biochemistry, Biomedical Institute, UNIRIO, 20211-040, Brazil.; Cell and Molecular Biology Graduate Program, UNIRIO, 20211-040, Brazil. |
| المصدر: | Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2022 Sep 02; Vol. 2022, pp. 2187696. Date of Electronic Publication: 2022 Sep 02 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Pub. Corp Country of Publication: United States NLM ID: 101479826 Publication Model: eCollection Cited Medium: Internet ISSN: 1942-0994 (Electronic) Linking ISSN: 19420994 NLM ISO Abbreviation: Oxid Med Cell Longev Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : New York : Hindawi Pub. Corp. Original Publication: 2008-2010: Austin, TX : Landes Bioscience |
| مواضيع طبية MeSH: | Lactoferrin*/metabolism , Lactoferrin*/pharmacology , Prostatic Neoplasms*/drug therapy , Prostatic Neoplasms*/metabolism, Apoptosis ; Cell Survival ; Humans ; Male ; Reactive Oxygen Species/metabolism |
| مستخلص: | Bovine lactoferrin (bLf) is a multifunctional protein widely associated with anticancer activity. Prostate cancer is the second most frequent type of cancer worldwide. This study was aimed at evaluating the influence of bLf on cell viability, cell cycle progression, reactive oxygen species (ROS) production, and rate of apoptosis in the human prostate cancer cell line (DU-145). MTT assay and trypan blue exclusion were used to analyze cell viability. Morphological changes were analyzed through optical microscopy after 24 h and 48 h of bLf treatment. FITC-bLf internalization and cellular damage were observed within 24 h by confocal fluorescence microscopy. Cell cycle analyses were performed by flow cytometry and propidium iodide. For caspases 3/7 activation and reactive oxygen species production evaluation, cells were live-imaged using the high-throughput system Operetta. The cell viability assays demonstrated that bLf induces cell death and morphological changes after 24 h and 48 h of treatment compared to control on DU-145 cells. The bLf internalization was detected in DU-145 cells, G Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper. (Copyright © 2022 Vanessa P. Rocha et al.) |
| References: | Exp Oncol. 2018 Oct;40(3):184-189. (PMID: 30285009) Drug Deliv. 2020 Dec;27(1):1236-1247. (PMID: 32812454) Biofactors. 2000;12(1-4):83-8. (PMID: 11216510) Jpn J Cancer Res. 2000 Jun;91(6):582-8. (PMID: 10874209) Nutr Cancer. 2014;66(8):1371-85. (PMID: 25356800) Front Biosci (Schol Ed). 2011 Jun 01;3(3):1080-8. (PMID: 21622257) Proc Natl Acad Sci U S A. 2011 May 24;108(21):8773-8. (PMID: 21555567) Ann Allergy Asthma Immunol. 1997 Mar;78(3):245-9; quiz 249-50. (PMID: 9087147) Science. 1998 Aug 28;281(5381):1309-12. (PMID: 9721092) Biochimie. 2009 Jan;91(1):3-10. (PMID: 18541155) Biochem Cell Biol. 2017 Feb;95(1):99-109. (PMID: 28169560) Front Oncol. 2018 Jun 04;8:200. (PMID: 29915723) Heliyon. 2021 Sep 28;7(9):e08087. (PMID: 34632151) J Dairy Sci. 2009 Jun;92(6):2477-84. (PMID: 19447979) J Med Virol. 2004 Oct;74(2):262-71. (PMID: 15332275) Cell. 2011 Mar 4;144(5):646-74. (PMID: 21376230) PLoS One. 2018 Jan 30;13(1):e0191683. (PMID: 29381751) Jpn J Cancer Res. 1999 Mar;90(3):262-7. (PMID: 10359039) J Dairy Sci. 2013;96(12):7511-20. (PMID: 24140317) Methods Mol Biol. 2018;1683:47-57. (PMID: 29082486) Clin Exp Metastasis. 1999 Feb;17(1):35-40. (PMID: 10390145) CA Cancer J Clin. 2022 Jan;72(1):7-33. (PMID: 35020204) Virology. 2014 Mar;452-453:297-302. (PMID: 24606707) BMC Cancer. 2015 May 22;15:425. (PMID: 25998617) CA Cancer J Clin. 2021 May;71(3):209-249. (PMID: 33538338) J Dairy Sci. 2010 Jun;93(6):2344-50. (PMID: 20494139) J Immunol Methods. 1983 Dec 16;65(1-2):55-63. (PMID: 6606682) Biochem J. 1995 Nov 15;312 ( Pt 1):107-14. (PMID: 7492299) Psychol Rev. 2004 Jan;111(1):33-51. (PMID: 14756584) Genes Dev. 2018 Sep 1;32(17-18):1105-1140. (PMID: 30181359) Exp Oncol. 2017 Jul;39(2):106-111. (PMID: 29483488) Cancer Biol Ther. 2002 May-Jun;1(3):226-31. (PMID: 12432268) Annu Rev Biochem. 1999;68:383-424. (PMID: 10872455) J Med Virol. 2003 Apr;69(4):495-502. (PMID: 12601757) Biochem Cell Biol. 2021 Feb;99(1):149-158. (PMID: 33307991) Protein Pept Lett. 2013 Apr;20(4):450-8. (PMID: 23016584) Crit Rev Food Sci Nutr. 2009 Mar;49(3):203-17. (PMID: 19093266) Oxid Med Cell Longev. 2019 Jun 3;2019:6797921. (PMID: 31281587) Food Chem. 2013 Dec 1;141(3):3007-13. (PMID: 23871052) J Vet Med Sci. 2008 May;70(5):443-8. (PMID: 18525164) Cell Mol Life Sci. 2005 Nov;62(22):2588-98. (PMID: 16261252) Sci Rep. 2016 Aug 31;6:32062. (PMID: 27576789) Science. 1998 Aug 28;281(5381):1305-8. (PMID: 9721089) Biomed Pharmacother. 2017 Dec;96:1109-1118. (PMID: 29196103) J Mol Biol. 1997 Nov 28;274(2):222-36. (PMID: 9398529) Arch Virol. 2021 Apr;166(4):1203-1211. (PMID: 33606112) Virchows Arch B Cell Pathol. 1977 Aug 10;24(3):227-42. (PMID: 410154) Hum Exp Toxicol. 2018 Sep;37(9):953-958. (PMID: 29226720) Adv Nutr. 2011 Nov;2(6):472-85. (PMID: 22332090) Biomaterials. 2014 Aug;35(26):7522-34. (PMID: 24933511) Cell Biochem Funct. 2017 Jan;35(1):33-41. (PMID: 28052347) |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) EC 3.4.21.- (Lactoferrin) |
| تواريخ الأحداث: | Date Created: 20220912 Date Completed: 20220913 Latest Revision: 20220913 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9463017 |
| DOI: | 10.1155/2022/2187696 |
| PMID: | 36092155 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1942-0994 |
|---|---|
| DOI: | 10.1155/2022/2187696 |