دورية أكاديمية
Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC).
| العنوان: | Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC). |
|---|---|
| المؤلفون: | Sauter M; Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany. Electronic address: manuela.sauter@uksh.de., Sauter RJ; Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany., Nording H; Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany., Olbrich M; University Hospital, Department of Cardiology, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany., Emschermann F; University Hospital, Department of Cardiology, Eberhard Karls University Tuebingen, 72076 Tuebingen, Germany., Langer HF; Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany; Department of Cardiology, Angiology, Haemostaseology and Medical Intensive Care, University Medical Centre Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany. Electronic address: harald.langer@uksh.de. |
| المصدر: | STAR protocols [STAR Protoc] 2022 Sep 16; Vol. 3 (3), pp. 101664. Date of Electronic Publication: 2022 Sep 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Bone Marrow* , Dendritic Cells*, Animals ; Apolipoproteins E ; Bone Marrow Cells ; CD11c Antigen ; Mice |
| مستخلص: | Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c + cells and CD11c + -derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in culture dishes, treating them with differentiation factors, and collecting cells after removal of undesirable populations. This protocol can be adapted for CD11c + cells in different contexts, thus, serving as models for different diseases and to analyze cell-specific molecules. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021). Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell biology; Cell culture; Cell isolation; Flow cytometry/Mass cytometry; Health sciences; Immunology; Molecular biology |
| المشرفين على المادة: | 0 (Apolipoproteins E) 0 (CD11c Antigen) |
| تواريخ الأحداث: | Date Created: 20220913 Date Completed: 20220922 Latest Revision: 20220930 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9471451 |
| DOI: | 10.1016/j.xpro.2022.101664 |
| PMID: | 36097382 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
|---|---|
| DOI: | 10.1016/j.xpro.2022.101664 |