دورية أكاديمية
Aptamer-based enzyme-linked oligonucleotide assay for specific detection of clinical bacterial strains isolated from cerebrospinal fluid samples.
| العنوان: | Aptamer-based enzyme-linked oligonucleotide assay for specific detection of clinical bacterial strains isolated from cerebrospinal fluid samples. |
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| المؤلفون: | Pourdadashi A; Department of Biology, Shahed University, Tehran-Qom Express way, Tehran 3319118651, Iran., Rezaei Adriani R; Department of Biology, Shahed University, Tehran-Qom Express way, Tehran 3319118651, Iran., Mousavi Gargari SL; Department of Biology, Shahed University, Tehran-Qom Express way, Tehran 3319118651, Iran. Electronic address: slmousavi@shahed.ac.ir. |
| المصدر: | Journal of bioscience and bioengineering [J Biosci Bioeng] 2022 Nov; Vol. 134 (5), pp. 441-449. Date of Electronic Publication: 2022 Sep 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Society for Biotechnology, Japan Country of Publication: Japan NLM ID: 100888800 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1347-4421 (Electronic) Linking ISSN: 13474421 NLM ISO Abbreviation: J Biosci Bioeng Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003-: Osaka, Japan : Society for Biotechnology, Japan Original Publication: Osaka, Japan : Amsterdam, The Netherlands : Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science, 1999- |
| مواضيع طبية MeSH: | Aptamers, Nucleotide*/genetics , Meningitis, Bacterial*/diagnosis, Humans ; SELEX Aptamer Technique/methods ; DNA, Single-Stranded ; Bacteria/metabolism |
| مستخلص: | Meningitis, acute infection of the meninges, is the 10th leading cause of mortality among infectious diseases. Although many different causes for meningitis (viruses and bacteria) have been diagnosed, the most common ones are Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae. The effort to find a new method for detection of bacterial meningitis is an urgent need for clinical treatment. DNA aptamers generated by cell-systematic evolution of ligands by exponential enrichment (SELEX) against bacterial cells provide a novel cell labeling and biosensing technique. Here, we isolated single-stranded DNA aptamers during the SELEX method with a high affinity for different bacterial genera. This approach was demonstrated on H. influenzae type B, N. meningitidis serogroups A, B, C, and Y, and Streptococcus pneumoniae serotypes 18, 14, 19A, 6A, and 6B which served as targets in 20 rounds of cell-SELEX. After 20 rounds of SELEX, a total of 93 aptamers were identified. Among these, aptamers C65 and C50 showed the highest affinity toward targets with a dissociation constant of 6.98 and 15.79, respectively. Selected aptamers were able to successfully detect clinical bacterial strains isolated from cerebrospinal fluid samples of meningitis patients by double-aptamer sandwich enzyme-linked oligonucleotide assay (ELONA). Our findings demonstrated that aptamers with broad affinity to bacterial taxa in different genera can be isolated for the development of diagnostic tools for multiple targets. We further showed that sandwich ELONA based on single-stranded DNA aptamer is sensitive and specific enough for detection of the superior cause of bacterial meningitis. (Copyright © 2022 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aptamer; Enzyme-linked oligonucleotide assay; Flow cytometry; Meningitis; Systematic evolution of ligands by exponential enrichment |
| المشرفين على المادة: | 0 (DNA, Single-Stranded) 0 (Aptamers, Nucleotide) |
| تواريخ الأحداث: | Date Created: 20220915 Date Completed: 20221121 Latest Revision: 20221121 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jbiosc.2022.07.009 |
| PMID: | 36109302 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1347-4421 |
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| DOI: | 10.1016/j.jbiosc.2022.07.009 |