دورية أكاديمية
أعرض في EDS

Photodynamic inactivation of Staphylococcus aureus in the presence of aggregation-prone photosensitizers based on BODIPY used at submicromolar concentrations.

التفاصيل البيبلوغرافية
العنوان: Photodynamic inactivation of Staphylococcus aureus in the presence of aggregation-prone photosensitizers based on BODIPY used at submicromolar concentrations.
المؤلفون: Navarro-Barreda D; Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain., de Llanos R; Unidad Predepartamental de Medicina, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain., Miravet JF; Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain., Galindo F; Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain. Electronic address: francisco.galindo@uji.es.
المصدر: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2022 Oct; Vol. 235, pp. 112543. Date of Electronic Publication: 2022 Aug 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Sequoia Country of Publication: Switzerland NLM ID: 8804966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2682 (Electronic) Linking ISSN: 10111344 NLM ISO Abbreviation: J Photochem Photobiol B Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Elsevier Sequoia, 1987-
مواضيع طبية MeSH: Photochemotherapy* , Staphylococcal Infections*, Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Boron Compounds ; Coloring Agents/pharmacology ; Escherichia coli ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Humans ; Lysine/pharmacology ; Photosensitizing Agents/chemistry ; Photosensitizing Agents/pharmacology ; Singlet Oxygen/chemistry ; Solvents/pharmacology ; Staphylococcus aureus ; Valine/pharmacology
مستخلص: Two new brominated BODIPYs (1 and 2) bearing amino acid-based chains (l-valine for 1, and dimethyl-l-lysine for 2) were synthesized and characterized. In organic solvents, 1 and 2 were fully soluble and showed the photophysical properties expected for brominated BODIPY dyes, including efficient generation of singlet oxygen ( 1 O 2 ), upon irradiation. In contrast, in aqueous media, both compounds were prone to aggregation and the photo-induced generation of 1 O 2 was halted. Despite the lack of generation of this reactive species in aqueous media (in cuvette), both 1 and 2 have positive antimicrobial Photodynamic Inactivation (aPDI) effect. The activity against gram-positive Staphylococcus aureus and gram-negative Escherichia coli was determined through the inactivation curves, with a total energy dose of 5.3 J/cm 2 (white light LED used as an energy source). Compound 2 was highly active against both gram-positive and gram-negative bacteria (3 log CFU/mL reduction was obtained at 0.16 μM for S. aureus and 2.5-5.0 μM for E. coli), whereas 1 was less effective to kill S. aureus (3 log CFU/mL at 0.32 μM) and ineffective for E. coli. The higher efficiency of 2, as compared to 1, to reduce the population of bacteria, can reside in the presence of a protonatable residue in 2, allowing a more effective interaction of this molecule with the cell walls of the microorganisms. In order to explain the lack of reactivity in pure aqueous media (in cuvette) and the contrasting good activity in the presence of bacterial cells it can be hypothesized that upon interaction with the walls of the microorganisms, the aggregated photosensitizers suffer a disaggregation process restoring the ability to generate 1 O 2 , and hence leading to efficient photodynamic activity against these pathogenic microorganisms, in agreement with the similar effect observed recently for porphyrinoid photosensitizers.
Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest to declare.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: E. coli; ESKAPE; Photo-antimicrobials; Photodynamic inactivation; Photosensitizers; S. aureus; Singlet oxygen
المشرفين على المادة: 0 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
0 (Anti-Bacterial Agents)
0 (Boron Compounds)
0 (Coloring Agents)
0 (Photosensitizing Agents)
0 (Solvents)
17778-80-2 (Singlet Oxygen)
HG18B9YRS7 (Valine)
K3Z4F929H6 (Lysine)
تواريخ الأحداث: Date Created: 20220916 Date Completed: 20220929 Latest Revision: 20220929
رمز التحديث: 20231215
DOI: 10.1016/j.jphotobiol.2022.112543
PMID: 36113260
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2682
DOI:10.1016/j.jphotobiol.2022.112543