دورية أكاديمية
Detection of Leptospira spp. in genitourinary tract of female goats managed in the brazilian semiarid.
| العنوان: | Detection of Leptospira spp. in genitourinary tract of female goats managed in the brazilian semiarid. |
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| المؤلفون: | Soares RR; Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Av. Universitária, s/n, Santa Cecília, Patos, PB, 58708-110, Brazil., Natércio da Costa Barnabé N; Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Av. Universitária, s/n, Santa Cecília, Patos, PB, 58708-110, Brazil., Cristiny Rodrigues Silva ML; Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Av. Universitária, s/n, Santa Cecília, Patos, PB, 58708-110, Brazil., Figueiredo da Costa D; Universidade Federal da Paraíba (UFPB), Centro de Ciências Agrárias (CCA), Rodovia BR 079, Km 02, Areia, PB, 58397-000, Brazil., Araújo Júnior JP; Universidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, campus de Botucatu, Botucatu, SP, 18618-687, Brazil., Malossi CD; Universidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, campus de Botucatu, Botucatu, SP, 18618-687, Brazil., Ullmann LS; Universidade Estadual Paulista (Unesp), Av. Prof. Mário Rubens Guimarães Montenegro, s/n, campus de Botucatu, Botucatu, SP, 18618-687, Brazil., Silvano Dos Santos Higino S; Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Av. Universitária, s/n, Santa Cecília, Patos, PB, 58708-110, Brazil., Santos de Azevedo S; Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Av. Universitária, s/n, Santa Cecília, Patos, PB, 58708-110, Brazil., Alves CJ; Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Av. Universitária, s/n, Santa Cecília, Patos, PB, 58708-110, Brazil. Electronic address: clebertja@uol.com.br. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2022 Nov; Vol. 172, pp. 105763. Date of Electronic Publication: 2022 Sep 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Leptospira* , Leptospirosis*/diagnosis , Leptospirosis*/epidemiology , Leptospirosis*/veterinary , Urinary Tract*, Animals ; Female ; Brazil/epidemiology ; Goats ; Serogroup |
| مستخلص: | Studies related to the prevalence of leptospirosis in the semiarid region showed that even during long periods of drought, the disease has a remarkable frequency in herds in the region. It is a neglected disease and the extent of its effects in the Brazilian semiarid region is not known. The dynamics of this agent is well studied in the urinary tract, however, there are not many studies regarding the genital tract in female goats. Observing this scenario, the present work aimed to diagnose Leptospira spp. in female goats kept in the Brazilian semiarid region by means of serological, molecular and isolation techniques. Blood samples, vaginal fluid, urine and fragments of organs from the genitourinary tract were collected from 40 goats destined for slaughter. Microscopic agglutination test (MAT) was used as a serological technique, with a battery of 24 serovars. The Polymerase Chain Reaction (PCR) of the vaginal fluid, urine and organ fragments was performed, as well as the bacterial growth of these same products in a selective medium. Isolation positive samples were subjected to PCR. It was observed that two (5%) animals were serologically positive for the Pyrogenes serogroup. A total of 29 (72.5%) animals were PCR positive, with DNA present in 51/160 (31.8%) samples from the genital tract and 34/120 (28.3%) from the urinary tract, with no statistical difference. For bacterial growth, 22/40 (55%) animals were positive for growth, with morphology being observed in 19/160 (11.8%) for the genital tract and 16/120 (13.3%) for the urinary tract, with no statistical difference. Two uterus samples showed 99% similarity with L. interrogans after sequencing. Thus, female goats kept under semiarid conditions were positive for Leptospira spp, with positive samples from both the urinary and genital tracts, which possible is an alternative way of adapting and maintaining the agent for severe and adverse conditions. (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Genital tract; Goat leptospirosis; Isolation; PCR; Venereal transmission |
| تواريخ الأحداث: | Date Created: 20220918 Date Completed: 20221027 Latest Revision: 20221027 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.micpath.2022.105763 |
| PMID: | 36116606 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2022.105763 |