دورية أكاديمية
أعرض في EDS

Characterization and assessment of lung and bone marrow derived endothelial cells and their bone regenerative potential.

التفاصيل البيبلوغرافية
العنوان: Characterization and assessment of lung and bone marrow derived endothelial cells and their bone regenerative potential.
المؤلفون: Moraes de Lima Perini M; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, United States., Valuch CR; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, United States., Dadwal UC; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Awosanya OD; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Mostardo SL; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Blosser RJ; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Knox AM; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., McGuire AC; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Battina HL; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Nazzal M; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Kacena MA; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States.; Richard L. Roudebush Veterans Affairs (VA) Medical Center, Indianapolis, IN, United States., Li J; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, United States.
المصدر: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Aug 31; Vol. 13, pp. 935391. Date of Electronic Publication: 2022 Aug 31 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Endothelial Cells* , Fractures, Bone*, Animals ; Bone Marrow ; Bone Regeneration ; Collagen ; Disease Models, Animal ; Lung ; Mice ; Rats
مستخلص: Angiogenesis is important for successful fracture repair. Aging negatively affects the number and activity of endothelial cells (ECs) and subsequently leads to impaired bone healing. We previously showed that implantation of lung-derived endothelial cells (LECs) improved fracture healing in rats. In this study, we characterized and compared neonatal lung and bone marrow-derived endothelial cells (neonatal LECs and neonatal BMECs) and further asses3sed if implantation of neonatal BMECs could enhance bone healing in both young and aged mice. We assessed neonatal EC tube formation, proliferation, and wound migration ability in vitro in ECs isolated from the bone marrow and lungs of neonatal mice. The in vitro studies demonstrated that both neonatal LECs and neonatal BMECs exhibited EC traits. To test the function of neonatal ECs in vivo , we created a femoral fracture in young and aged mice and implanted a collagen sponge to deliver neonatal BMECs at the fracture site. In the mouse fracture model, endochondral ossification was delayed in aged control mice compared to young controls. Neonatal BMECs significantly improved endochondral bone formation only in aged mice. These data suggest BMECs have potential to enhance aged bone healing. Compared to LECs, BMECs are more feasible for translational cell therapy and clinical applications in bone repair. Future studies are needed to examine the fate and function of BMECs implanted into the fracture sites.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Moraes de Lima Perini, Valuch, Dadwal, Awosanya, Mostardo, Blosser, Knox, McGuire, Battina, Nazzal, Kacena and Li.)
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معلومات مُعتمدة: I01 BX003751 United States BX BLRD VA; R01 AG060621 United States AG NIA NIH HHS; U54 DK106846 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: aging; angiogenesis; endothelial progenitor cells; fracture healing; mouse
المشرفين على المادة: 9007-34-5 (Collagen)
تواريخ الأحداث: Date Created: 20220919 Date Completed: 20220920 Latest Revision: 20221120
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9470942
DOI: 10.3389/fendo.2022.935391
PMID: 36120459
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-2392
DOI:10.3389/fendo.2022.935391