دورية أكاديمية
Characterization and assessment of lung and bone marrow derived endothelial cells and their bone regenerative potential.
العنوان: | Characterization and assessment of lung and bone marrow derived endothelial cells and their bone regenerative potential. |
---|---|
المؤلفون: | Moraes de Lima Perini M; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, United States., Valuch CR; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, United States., Dadwal UC; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Awosanya OD; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Mostardo SL; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Blosser RJ; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Knox AM; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., McGuire AC; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Battina HL; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Nazzal M; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States., Kacena MA; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN, United States.; Richard L. Roudebush Veterans Affairs (VA) Medical Center, Indianapolis, IN, United States., Li J; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, United States. |
المصدر: | Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Aug 31; Vol. 13, pp. 935391. Date of Electronic Publication: 2022 Aug 31 (Print Publication: 2022). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
مواضيع طبية MeSH: | Endothelial Cells* , Fractures, Bone*, Animals ; Bone Marrow ; Bone Regeneration ; Collagen ; Disease Models, Animal ; Lung ; Mice ; Rats |
مستخلص: | Angiogenesis is important for successful fracture repair. Aging negatively affects the number and activity of endothelial cells (ECs) and subsequently leads to impaired bone healing. We previously showed that implantation of lung-derived endothelial cells (LECs) improved fracture healing in rats. In this study, we characterized and compared neonatal lung and bone marrow-derived endothelial cells (neonatal LECs and neonatal BMECs) and further asses3sed if implantation of neonatal BMECs could enhance bone healing in both young and aged mice. We assessed neonatal EC tube formation, proliferation, and wound migration ability in vitro in ECs isolated from the bone marrow and lungs of neonatal mice. The in vitro studies demonstrated that both neonatal LECs and neonatal BMECs exhibited EC traits. To test the function of neonatal ECs in vivo , we created a femoral fracture in young and aged mice and implanted a collagen sponge to deliver neonatal BMECs at the fracture site. In the mouse fracture model, endochondral ossification was delayed in aged control mice compared to young controls. Neonatal BMECs significantly improved endochondral bone formation only in aged mice. These data suggest BMECs have potential to enhance aged bone healing. Compared to LECs, BMECs are more feasible for translational cell therapy and clinical applications in bone repair. Future studies are needed to examine the fate and function of BMECs implanted into the fracture sites. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Moraes de Lima Perini, Valuch, Dadwal, Awosanya, Mostardo, Blosser, Knox, McGuire, Battina, Nazzal, Kacena and Li.) |
References: | Bone Rep. 2017 Mar 16;6:87-100. (PMID: 28377988) Nano Lett. 2019 May 8;19(5):3040-3048. (PMID: 30968694) J Orthop Res. 2019 Jan;37(1):35-50. (PMID: 30370699) Blood. 2004 Nov 1;104(9):2752-60. (PMID: 15226175) Bone. 2021 May;146:115883. (PMID: 33581374) Arch Biochem Biophys. 2014 Nov 1;561:109-17. (PMID: 25034215) Stem Cells Int. 2017;2017:7923826. (PMID: 28523072) J Acquir Immune Defic Syndr. 2015 Apr 15;68(5):550-3. (PMID: 25564108) Nat Rev Mol Cell Biol. 2007 Jun;8(6):464-78. (PMID: 17522591) Tissue Eng Part B Rev. 2018 Feb;24(1):1-24. (PMID: 28548628) Stem Cells. 2011 Nov;29(11):1898-907. (PMID: 21905169) Ann Intern Med. 2017 Aug 1;167(3):ITC17-ITC32. (PMID: 28761958) Biomaterials. 2013 Nov;34(35):8887-98. (PMID: 23958029) J Orthop Trauma. 2014;28 Suppl 1:S24-7. (PMID: 24464097) Nat Genet. 2007 Jul;39(7):875-81. (PMID: 17558409) Am J Respir Cell Mol Biol. 2015 Oct;53(4):500-12. (PMID: 25719275) BMC Med Genomics. 2010 May 13;3:18. (PMID: 20465783) Stem Cells Int. 2018 Nov 18;2018:9847015. (PMID: 30581475) Bone. 2016 Oct;91:30-8. (PMID: 27353702) Nature. 2016 Oct 13;538(7624):260-264. (PMID: 27698416) Sci Adv. 2020 Nov 18;6(47):. (PMID: 33208358) Calcif Tissue Int. 2011 Nov;89(5):347-57. (PMID: 21882012) Int J Mol Sci. 2021 Oct 14;22(20):. (PMID: 34681756) Rom J Morphol Embryol. 2018;59(2):455-467. (PMID: 30173249) Yale J Biol Med. 2014 Dec 12;87(4):549-61. (PMID: 25506287) Nat Med. 2001 Apr;7(4):430-6. (PMID: 11283669) Cell Commun Signal. 2011 May 14;9:12. (PMID: 21569606) Stem Cell Res Ther. 2019 Jan 11;10(1):12. (PMID: 30635031) Toxicol Lett. 2020 Nov 1;334:110-116. (PMID: 32707277) Cell Physiol Biochem. 2018;49(2):555-564. (PMID: 30165361) JAKSTAT. 2013 Jul 1;2(3):e23930. (PMID: 24069548) Genes Cancer. 2011 Dec;2(12):1097-105. (PMID: 22866201) Arch Intern Med. 2003 Oct 13;163(18):2237-46. (PMID: 14557222) Bioact Mater. 2021 Dec 17;14:169-181. (PMID: 35310361) Bone. 2008 May;42(5):932-41. (PMID: 18326482) Sci Rep. 2018 Mar 1;8(1):3867. (PMID: 29497100) Spine J. 2011 Jun;11(6):506. (PMID: 21729800) Tissue Eng Part A. 2019 Dec;25(23-24):1623-1634. (PMID: 30973074) Handb Exp Pharmacol. 2007;(180):67-88. (PMID: 17554505) Stem Cell Res Ther. 2019 Jan 11;10(1):19. (PMID: 30635045) Stem Cells Transl Med. 2017 May;6(5):1316-1320. (PMID: 28296182) Biol Reprod. 2019 May 1;100(5):1228-1237. (PMID: 30601943) Bone Joint Res. 2018 May 5;7(3):232-243. (PMID: 29922441) Lab Animal Sci Prof. 2018 Sep;6(3):65-67. (PMID: 32025533) World J Stem Cells. 2019 Jun 26;11(6):281-296. (PMID: 31293713) Spine J. 2011 Jun;11(6):471-91. (PMID: 21729796) Pulm Circ. 2018 Jan-Mar;8(1):2045893217743950. (PMID: 29099663) J Orthop Res. 2009 Jan;27(1):8-14. (PMID: 18634016) J Am Heart Assoc. 2015 Jun 16;4(6):e002043. (PMID: 26080813) Bioact Mater. 2021 Nov 03;13:300-311. (PMID: 35224310) J Orthop Res. 2022 Feb;40(2):439-448. (PMID: 33713476) Int J Mol Sci. 2019 Nov 19;20(22):. (PMID: 31752267) J Bone Joint Surg Am. 2013 Sep 4;95(17):1537-45. (PMID: 24005193) J Orthop Res. 2010 Aug;28(8):1007-14. (PMID: 20135674) Cell Adh Migr. 2014;8(5):440-51. (PMID: 25482647) |
معلومات مُعتمدة: | I01 BX003751 United States BX BLRD VA; R01 AG060621 United States AG NIA NIH HHS; U54 DK106846 United States DK NIDDK NIH HHS |
فهرسة مساهمة: | Keywords: aging; angiogenesis; endothelial progenitor cells; fracture healing; mouse |
المشرفين على المادة: | 9007-34-5 (Collagen) |
تواريخ الأحداث: | Date Created: 20220919 Date Completed: 20220920 Latest Revision: 20221120 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9470942 |
DOI: | 10.3389/fendo.2022.935391 |
PMID: | 36120459 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!