دورية أكاديمية
Rapid Quantitation of Anatoxins in Benthic Cyanobacterial Mats Using Direct Analysis in Real-Time-High-Resolution Tandem Mass Spectrometry.
| العنوان: | Rapid Quantitation of Anatoxins in Benthic Cyanobacterial Mats Using Direct Analysis in Real-Time-High-Resolution Tandem Mass Spectrometry. |
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| المؤلفون: | Beach DG; Biotoxin Metrology, National Research Council Canada, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada., Bruce M; Canadian Rivers Institute, University of New Brunswick, P.O. Box 4400, Fredericton, New Brunswick E3B 5A3, Canada., Lawrence J; Department of Biology, University of New Brunswick, 10 Bailey Drive, Fredericton, New Brunswick E3B 5A3, Canada., McCarron P; Biotoxin Metrology, National Research Council Canada, 1411 Oxford Street, Halifax, Nova Scotia B3H 3Z1, Canada. |
| المصدر: | Environmental science & technology [Environ Sci Technol] 2022 Oct 04; Vol. 56 (19), pp. 13837-13844. Date of Electronic Publication: 2022 Sep 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0213155 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5851 (Electronic) Linking ISSN: 0013936X NLM ISO Abbreviation: Environ Sci Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington DC : American Chemical Society Original Publication: Easton, Pa. : American Chemical Society, c1967- |
| مواضيع طبية MeSH: | Cyanobacteria*/chemistry , Tandem Mass Spectrometry*, Animals ; Cyanobacteria Toxins ; Neurotoxins ; Rivers/chemistry ; Tropanes |
| مستخلص: | Toxic benthic cyanobacterial mats are increasingly reported worldwide as being responsible for animal mortalities due to their production of the potent neurotoxin anatoxin-a (ATX) and its analogues. Improved analytical methods for anatoxins are needed to address public health and watershed management challenges arising from extremely high spatial and temporal variability within impacted systems. We present the development, validation, and application of a direct analysis in real-time-high-resolution tandem mass spectrometry (DART-HRMS/MS) method for analysis of anatoxins in cyanobacterial field samples, including a simplified sample preparation approach. The method showed excellent sensitivity and selectivity for ATX, homoanatoxin-a, and dihydroanatoxin-a. Isotopically labeled ATX was used as an internal standard for all three analogues and successfully corrected for the matrix effects observed (86 ± 16% suppression). The limit of detection and recovery for ATX was estimated as 5 ng/g and 88%, respectively, using spiked samples. The total analysis time was ∼2 min, and excellent agreement was observed with results from a liquid chromatography-HRMS reference method. Finally, the DART-HRMS/MS method was applied to a set of 45 Microcoleus -dominated benthic cyanobacterial mat samples from the Wolastoq near Fredericton, Canada, demonstrating its power and applicability in enabling broad-scale field studies of ATX distribution. |
| References: | Sci Rep. 2019 Jul 26;9(1):10894. (PMID: 31350418) Mar Drugs. 2017 Oct 11;15(10):. (PMID: 29019928) Toxicon. 2010 May;55(5):965-72. (PMID: 19878689) Environ Sci Technol. 2007 Jan 15;41(2):506-10. (PMID: 17310714) Rapid Commun Mass Spectrom. 2021 Jan 15;35(1):e8940. (PMID: 32881159) Talanta. 2015 Jan;132:836-44. (PMID: 25476385) Toxicon. 2005 Jun 1;45(7):919-28. (PMID: 15904687) Arch Microbiol. 2007 May;187(5):397-413. (PMID: 17186222) Harmful Algae. 2016 May;55:282-294. (PMID: 28073542) Toxicon. 2012 Sep 1;60(3):378-84. (PMID: 22534073) Food Addit Contam. 2001 Jun;18(6):525-31. (PMID: 11407751) Toxins (Basel). 2012 Oct;4(10):900-12. (PMID: 23162704) Toxicon. 2013 Jan;61:165-74. (PMID: 23159398) Toxicon. 2010 Nov;56(6):964-71. (PMID: 20615427) Toxicon. 2008 Jun 1;51(7):1308-15. (PMID: 18420244) Front Microbiol. 2020 Feb 06;11:129. (PMID: 32117151) Freshw Biol. 2020 Oct 1;65(10):1824-1842. (PMID: 34970014) Toxicon. 2008 Jul;52(1):163-74. (PMID: 18617214) PLoS One. 2018 May 18;13(5):e0197669. (PMID: 29775481) Chemosphere. 2021 Jan;263:127937. (PMID: 32828056) Anal Bioanal Chem. 2017 Aug;409(21):4959-4969. (PMID: 28634756) Anal Bioanal Chem. 2015 Jul;407(18):5353-63. (PMID: 25929442) Toxicon. 2018 Aug;150:13-16. (PMID: 29746979) Toxicon. 2012 Dec 15;60(8):1404-14. (PMID: 23085422) |
| فهرسة مساهمة: | Keywords: DART−HRMS; Microcoleus; Phormidium; ambient ionization; cyanotoxins; high-throughput screening |
| المشرفين على المادة: | 0 (Cyanobacteria Toxins) 0 (Neurotoxins) 0 (Tropanes) 80023A73NK (anatoxin a) |
| تواريخ الأحداث: | Date Created: 20220920 Date Completed: 20221005 Latest Revision: 20221027 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9535865 |
| DOI: | 10.1021/acs.est.2c05426 |
| PMID: | 36125920 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5851 |
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| DOI: | 10.1021/acs.est.2c05426 |