دورية أكاديمية
Activation of Neutrophils by Mucin-Vaterite Microparticles.
| العنوان: | Activation of Neutrophils by Mucin-Vaterite Microparticles. |
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| المؤلفون: | Mikhalchik E; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Basyreva LY; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Gusev SA; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Panasenko OM; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Klinov DV; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.; Laboratory of Biomaterials, Sirius University of Science and Technology, 354340 Sochi, Russia., Barinov NA; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.; Laboratory of Biomaterials, Sirius University of Science and Technology, 354340 Sochi, Russia., Morozova OV; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.; National Research Center of Epidemiology and Microbiology of N.F. Gamaleya, 123098 Moscow, Russia., Moscalets AP; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.; Laboratory of Biomaterials, Sirius University of Science and Technology, 354340 Sochi, Russia., Maltseva LN; Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia., Filatova LY; Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia., Pronkin EA; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Bespyatykh JA; Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.; Expertise Department in Anti-Doping and Drug Control, Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia., Balabushevich NG; Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2022 Sep 13; Vol. 23 (18). Date of Electronic Publication: 2022 Sep 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Luminol*/chemistry , Neutrophils*/metabolism, Calcium/metabolism ; Calcium Carbonate/pharmacology ; Calcium Oxalate/metabolism ; Interleukin-10/metabolism ; Interleukin-6/metabolism ; Interleukin-8/metabolism ; Ions/metabolism ; Magnesium/metabolism ; Mucins/metabolism ; Oxidants/pharmacology ; Phosphates/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Zymosan/pharmacology |
| مستخلص: | Nano- and microparticles enter the body through the respiratory airways and the digestive system, or form as biominerals in the gall bladder, salivary glands, urinary bladder, kidney, or diabetic pancreas. Calcium, magnesium, and phosphate ions can precipitate from biological fluids in the presence of mucin as hybrid nanoparticles. Calcium carbonate nanocrystallites also trap mucin and are assembled into hybrid microparticles. Both mucin and calcium carbonate polymorphs (calcite, aragonite, and vaterite) are known to be components of such biominerals as gallstones which provoke inflammatory reactions. Our study was aimed at evaluation of neutrophil activation by hybrid vaterite-mucin microparticles (CCM). Vaterite microparticles (CC) and CCM were prepared under standard conditions. The diameter of CC and CCM was 3.3 ± 0.8 µm and 5.8 ± 0.7 µm, with ƺ-potentials of -1 ± 1 mV and -7 ± 1 mV, respectively. CC microparticles injured less than 2% of erythrocytes in 2 h at 1.5 mg mL -1 , and no hemolysis was detected with CCM; this let us exclude direct damage of cellular membranes by microparticles. Activation of neutrophils was analyzed by luminol- and lucigenin-dependent chemiluminescence (Lum-CL and Luc-CL), by cytokine gene expression (IL-6, IL-8, IL-10) and release (IL-1β, IL-6, IL-8, IL-10, TNF-α), and by light microscopy of stained smears. There was a 10-fold and higher increase in the amplitude of Lum-CL and Luc-CL after stimulation of neutrophils with CCM relative to CC. Adsorption of mucin onto prefabricated CC microparticles also contributed to activation of neutrophil CL, unlike mucin adsorption onto yeast cell walls (zymosan); adsorbed mucin partially suppressed zymosan-stimulated production of oxidants by neutrophils. Preliminary treatment of CCM with 0.1-10 mM NaOCl decreased subsequent activation of Lum-CL and Luc-CL of neutrophils depending on the used NaOCl concentration, presumably because of the surface mucin oxidation. Based on the results of ELISA, incubation of neutrophils with CCM downregulated IL-6 production but upregulated that of IL-8. IL-6 and IL-8 gene expression in neutrophils was not affected by CC or CCM according to RT 2 -PCR data, which means that post-translational regulation was involved. Light microscopy revealed adhesion of CC and CCM microparticles onto the neutrophils; CCM increased neutrophil aggregation with a tendency to form neutrophil extracellular traps (NETs). We came to the conclusion that the main features of neutrophil reaction to mucin-vaterite hybrid microparticles are increased oxidant production, cell aggregation, and NET-like structure formation, but without significant cytokine release (except for IL-8). This effect of mucin is not anion-specific since particles of powdered kidney stone (mainly calcium oxalate) in the present study or calcium phosphate nanowires in our previous report also activated Lum-CL and Luc-CL response of neutrophils after mucin sorption. |
| References: | Sci Rep. 2018 Jan 19;8(1):1256. (PMID: 29352150) Biochim Biophys Acta. 1999 Oct 6;1447(1):77-92. (PMID: 10500247) Blood. 2017 Jun 8;129(23):3100-3110. (PMID: 28416510) Int J Gen Med. 2022 Jun 08;15:5513-5531. (PMID: 35702368) Biochem Biophys Res Commun. 2017 Aug 19;490(2):499-505. (PMID: 28624457) Sci Rep. 2017 Nov 30;7(1):16628. (PMID: 29192209) Atherosclerosis. 2008 Jan;196(1):98-105. (PMID: 17350022) Dig Dis Sci. 1993 May;38(5):909-15. (PMID: 8482190) Pediatr Surg Int. 2007 Mar;23(3):219-23. (PMID: 17225180) Nephron. 1999;81 Suppl 1:38-44. (PMID: 9873213) Eur J Immunol. 2019 Mar;49(3):386-397. (PMID: 30443903) J Colloid Interface Sci. 2019 Jun 1;545:330-339. (PMID: 30901672) J Lipid Res. 2002 Nov;43(11):1950-9. (PMID: 12401894) Nat Commun. 2016 Mar 11;7:10973. (PMID: 26964500) Biochem Biophys Res Commun. 1985 Jan 16;126(1):482-8. (PMID: 2982377) Chem Commun (Camb). 2015 Nov 14;51(88):15902-5. (PMID: 26376942) Curr Biol. 2014 May 19;24(10):R453-62. (PMID: 24845678) J Formos Med Assoc. 2021 Jan;120(1 Pt 1):5-24. (PMID: 31882261) Viszeralmedizin. 2015 Jun;31(3):156-61. (PMID: 26468308) Chem Biol Interact. 2020 Dec 1;332:109305. (PMID: 33130048) Mediators Inflamm. 2012;2012:849136. (PMID: 22481865) J Mater Sci Mater Med. 2009 Feb;20(2):621-31. (PMID: 18925363) Asian J Surg. 2013 Jan;36(1):26-35. (PMID: 23270822) Cell Tissue Res. 2004 Dec;318(3):545-51. (PMID: 15490242) Nat Methods. 2016 Jun;13(6):528-34. (PMID: 27135973) Bull Exp Biol Med. 2018 Jun;165(2):235-238. (PMID: 29923001) Biomedicines. 2019 Aug 28;7(3):. (PMID: 31466331) Front Immunol. 2013 Jan 21;3:424. (PMID: 23346086) J Clin Invest. 1957 Mar;36(3):449-55. (PMID: 13406059) Front Pharmacol. 2020 Sep 30;11:554353. (PMID: 33101020) Int J Nanomedicine. 2015 Mar 23;10:2273-93. (PMID: 25848250) Hepatobiliary Pancreat Dis Int. 2021 Feb;20(1):61-66. (PMID: 33341401) J Tissue Eng. 2021 Oct 26;12:20417314211041428. (PMID: 34721831) Cells. 2020 Sep 22;9(9):. (PMID: 32971767) J Biol Chem. 2018 Jun 1;293(22):8543-8553. (PMID: 29581231) Nat Rev Immunol. 2012 Jun 15;12(7):492-502. (PMID: 22699831) Macromol Biosci. 2022 Jul;22(7):e2200005. (PMID: 35489086) Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4930-4. (PMID: 16549762) Dig Dis Sci. 1997 Jun;42(6):1283-9. (PMID: 9201096) Front Immunol. 2019 Nov 06;10:2478. (PMID: 31781090) J Nanobiotechnology. 2015 Sep 04;13:53. (PMID: 26337452) N Engl J Med. 1989 Feb 9;320(6):365-76. (PMID: 2536474) Adv Colloid Interface Sci. 2014 May;207:306-24. (PMID: 24794739) Glycobiology. 1995 Feb;5(1):29-38. (PMID: 7539644) Immunity. 2019 Sep 17;51(3):443-450.e4. (PMID: 31422870) Sci Rep. 2017 Nov 3;7(1):15003. (PMID: 29101355) FEBS Open Bio. 2020 Feb;10(2):180-196. (PMID: 31785127) Nanomedicine (Lond). 2011 Jun;6(4):643-58. (PMID: 21506688) Small. 2013 Jul 8;9(13):2297-307. (PMID: 23255529) Nanomedicine. 2020 Nov;30:102293. (PMID: 32853784) Glycobiology. 1996 Mar;6(2):191-208. (PMID: 8727791) J Immunol Methods. 1987 Mar 12;97(2):209-13. (PMID: 3029229) J Lipid Res. 1998 Sep;39(9):1744-51. (PMID: 9741686) J Colloid Interface Sci. 2021 Jul 15;594:802-811. (PMID: 33794402) Micromachines (Basel). 2018 Jun 19;9(6):. (PMID: 30424240) |
| معلومات مُعتمدة: | 121041500039-8 Development Program of Lomonosov MSU and registration theme; 20-1500390 Russian Science Foundation |
| فهرسة مساهمة: | Keywords: chemiluminescence; cytokines; inflammation; microparticles; mucin; neutrophils; vaterite |
| المشرفين على المادة: | 0 (Interleukin-6) 0 (Interleukin-8) 0 (Ions) 0 (Mucins) 0 (Oxidants) 0 (Phosphates) 0 (Tumor Necrosis Factor-alpha) 130068-27-8 (Interleukin-10) 2612HC57YE (Calcium Oxalate) 5EXP385Q4F (Luminol) 9010-72-4 (Zymosan) H0G9379FGK (Calcium Carbonate) I38ZP9992A (Magnesium) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20220923 Date Completed: 20220926 Latest Revision: 20220928 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9501559 |
| DOI: | 10.3390/ijms231810579 |
| PMID: | 36142492 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms231810579 |