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Comparative Analyses of Complete Chloroplast Genomes and Karyotypes of Allotetraploid Iris koreana and Its Putative Diploid Parental Species ( Iris Series Chinenses , Iridaceae).

التفاصيل البيبلوغرافية
العنوان: Comparative Analyses of Complete Chloroplast Genomes and Karyotypes of Allotetraploid Iris koreana and Its Putative Diploid Parental Species ( Iris Series Chinenses , Iridaceae).
المؤلفون: Park I; Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Korea.; Department of Biology and Chemistry, Changwon National University, Changwon 51140, Korea., Choi B; Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Korea., Weiss-Schneeweiss H; Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, A-1030 Vienna, Austria., So S; Plant Conservation Center, Korea National Park Research Institute, 2 Baengnyeonsa-gil, Seolcheon-Myeon, Muju-gun 55557, Korea., Myeong HH; Korea National Park Research Institute, 171 Dangu-ro, Wonju-si 26441, Korea., Jang TS; Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2022 Sep 18; Vol. 23 (18). Date of Electronic Publication: 2022 Sep 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Genome, Chloroplast* , Iridaceae* , Iris Plant*/genetics, DNA, Ribosomal/genetics ; Diploidy ; In Situ Hybridization, Fluorescence ; Karyotype ; Nucleotides ; Phylogeny
مستخلص: The Iris series Chinenses in Korea comprises four species ( I. minutoaurea , I. odaesanensis , I. koreana, and I. rossii ), and the group includes some endangered species, owing to their high ornamental, economic, and conservation values. Among them, the putative allotetraploid, Iris koreana (2 n = 4 x = 50), is hypothesized to have originated from the hybridization of the diploids I. minutoaurea (2 n = 2 x = 22) and I. odaesanensis (2 n = 2 x = 28) based on morphological characters, chromosome numbers, and genome size additivity. Despite extensive morphological and molecular phylogenetical studies on the genus Iris , little is known about Korean irises in terms of their complete chloroplast (cp) genomes and molecular cytogenetics that involve rDNA loci evolution based on fluorescence in situ hybridization (FISH). This study reports comparative analyses of the karyotypes of the three Iris species ( I. koreana , I. odaesanensis , and I. minutoaurea ), with an emphasis on the 5S and 35S rDNA loci number and localization using FISH together with the genome size and chromosome number. Moreover, the cp genomes of the same individuals were sequenced and assembled for comparative analysis. The rDNA loci numbers, which were localized consistently at the same position in all species, and the chromosome numbers and genome size values of tetraploid Iris koreana (four 5S and 35S loci; 2 n = 50; 1C = 7.35 pg) were additively compared to its putative diploid progenitors, I. minutoaurea (two 5S and 35S loci; 2 n = 22; 1C = 3.71 pg) and I. odaesanensis (two 5S and 35S loci; 2 n = 28; 1C = 3.68 pg). The chloroplast genomes were 152,259-155,145 bp in length, and exhibited a conserved quadripartite structure. The Iris cp genomes were highly conserved and similar to other Iridaceae cp genomes. Nucleotide diversity analysis indicated that all three species had similar levels of genetic variation, but the cp genomes of I. koreana and I. minutoaurea were more similar to each other than to I. odaesanensis . Positive selection was inferred for psbK and ycf2 genes of the three Iris species. Phylogenetic analyses consistently recovered I. odaesanensis as a sister to a clade containing I. koreana and I. minutoaurea . Although the phylogenetic relationship, rDNA loci number, and localization, together with the genome size and chromosome number of the three species, allowed for the inference of I. minutoaurea as a putative maternal taxon and I. odaesanensis as a paternal taxon, further analyses involving species-specific molecular cytogenetic markers and genomic in situ hybridization are required to interpret the mechanisms involved in the origin of the chromosomal variation in Iris series Chinenses . This study contributes towards the genomic and chromosomal evolution of the genus Iris .
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معلومات مُعتمدة: NRF-2018R1C1B6003170 National Research Foundation of Korea; NRF-2021R1I1A2045820 National Research Foundation of Korea
فهرسة مساهمة: Keywords: FISH; Iris series Chinenses; allopolyploidy; chloroplast genome; rDNA loci
المشرفين على المادة: 0 (DNA, Ribosomal)
0 (Nucleotides)
تواريخ الأحداث: Date Created: 20220923 Date Completed: 20220926 Latest Revision: 20220928
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9504294
DOI: 10.3390/ijms231810929
PMID: 36142840
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms231810929