دورية أكاديمية
Mesenchymal stromal cell-derived extracellular vesicles afford neuroprotection by modulating PI3K/AKT pathway and calcium oscillations.
العنوان: | Mesenchymal stromal cell-derived extracellular vesicles afford neuroprotection by modulating PI3K/AKT pathway and calcium oscillations. |
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المؤلفون: | Turovsky EA; Institute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia., Golovicheva VV; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia., Varlamova EG; Institute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia., Danilina TI; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia., Goryunov KV; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Shevtsova YA; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia.; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234 Moscow, Russia., Pevzner IB; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Zorova LD; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Babenko VA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Evtushenko EA; Faculty of Biology, Lomonosov Moscow State University, Moscow 119234, Russia., Zharikova AA; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234 Moscow, Russia., Khutornenko AA; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Kovalchuk SI; Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; 117997 Moscow, Russia., Plotnikov EY; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Zorov DB; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Sukhikh GT; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia., Silachev DN; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 117997 Moscow, Russia. |
المصدر: | International journal of biological sciences [Int J Biol Sci] 2022 Aug 21; Vol. 18 (14), pp. 5345-5368. Date of Electronic Publication: 2022 Aug 21 (Print Publication: 2022). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Ivyspring International Country of Publication: Australia NLM ID: 101235568 Publication Model: eCollection Cited Medium: Internet ISSN: 1449-2288 (Electronic) Linking ISSN: 14492288 NLM ISO Abbreviation: Int J Biol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Lake Haven, N.S.W., Australia : Ivyspring International, c2004- |
مواضيع طبية MeSH: | Extracellular Vesicles*/metabolism , Hypoxia-Ischemia, Brain*/metabolism , Mesenchymal Stem Cells*/metabolism, Animals ; Calcium/metabolism ; Calcium Signaling ; Humans ; Infant, Newborn ; Inositol/metabolism ; Ischemia/therapy ; Neuroprotection ; Phosphatidylinositol 3-Kinases/metabolism ; Proteome/metabolism ; Proto-Oncogene Proteins c-akt/metabolism |
مستخلص: | Mesenchymal stromal cells (MSC) are widely recognized as potential effectors in neuroprotective therapy. The protective properties of MSC were considered to be associated with the secretion of extracellular vesicles (MSC-EV). We explored the effects of MSC-EV in vivo on models of traumatic and hypoxia-ischemia (HI) brain injury. Neuroprotective mechanisms triggered by MSC-EV were also studied in vitro using a primary neuroglial culture. Intranasal administration of MSC-EV reduced the volume of traumatic brain damage, correlating with a recovery of sensorimotor functions. Neonatal HI-induced brain damage was mitigated by the MSC-EV administration. This therapy also promoted the recovery of sensorimotor functions, implying enhanced neuroplasticity, and MSC-EV-induced growth of neurites in vitro supports this. In the in vitro ischemic model, MSC-EV prevented cell calcium (Ca 2+ ) overload and subsequent cell death. In mixed neuroglial culture, MSC-EV induced inositol trisphosphate (IP3) receptor-related Ca 2+ oscillations in astrocytes were associated with resistance to calcium overload not only in astrocytes but also in co-cultured neurons, demonstrating intercellular positive crosstalk between neural cells. This implies that phosphatidylinositol 3-Kinase/AKT signaling is one of the main pathways in MSC-EV-mediated protection of neural cells exposed to ischemic challenge. Components of this pathway were identified among the most enriched categories in the MSC-EV proteome. Competing Interests: Competing Interests: The authors have declared that no competing interest exists. (© The author(s).) |
References: | Function (Oxf). 2022 Jan 10;3(2):zqab069. (PMID: 35399497) Adv Funct Mater. 2020 Sep 10;30(37):. (PMID: 32952493) Proteomics. 2009 Nov;9(21):4997-5000. (PMID: 19810033) Biochimie. 2013 Dec;95(12):2271-85. (PMID: 23827856) FEBS Lett. 1996 Aug 26;392(2):143-7. (PMID: 8772192) J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094) Regen Med. 2010 Jan;5(1):121-43. (PMID: 20017699) Molecules. 2018 Mar 19;23(3):. (PMID: 29562677) Arch Neurol. 2007 Jun;64(6):794-800. (PMID: 17562927) Neurobiol Dis. 2021 Sep;157:105445. (PMID: 34271084) Biochemistry (Mosc). 2012 Sep;77(9):996-9. (PMID: 23157258) Biochem Soc Trans. 2018 Aug 20;46(4):843-853. (PMID: 29986939) Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):6291-6296. (PMID: 29844175) Eur J Neurol. 2003 Sep;10(5):471-7. (PMID: 12940825) Int J Proteomics. 2012;2012:971907. (PMID: 22852084) Front Neurol. 2020 Sep 18;11:812. (PMID: 33071923) Nat Protoc. 2007;2(1):187-90. (PMID: 17401329) Bull Exp Biol Med. 2016 Jan;160(3):313-8. (PMID: 26742738) Neurosci Bull. 2020 Jul;36(7):733-760. (PMID: 32219700) Mol Cell Neurosci. 2019 Apr;96:10-24. (PMID: 30776416) Brain Res. 2021 Mar 2;:147399. (PMID: 33662337) Neuropharmacology. 2000 Mar 3;39(5):777-87. (PMID: 10699444) Nat Protoc. 2009;4(1):44-57. (PMID: 19131956) Circ Res. 2017 May 12;120(10):1658-1673. (PMID: 28495996) Cell Mol Neurobiol. 2012 May;32(4):567-76. (PMID: 22290155) Mol Cell Proteomics. 2019 Feb;18(2):383-390. (PMID: 30373789) J Cell Biochem. 2011 Feb;112(2):374-80. (PMID: 21268056) Stem Cell Res Ther. 2020 Jul 22;11(1):313. (PMID: 32698909) Nucleic Acids Res. 2012 Jan;40(Database issue):D1241-4. (PMID: 21989406) Biochemistry (Mosc). 2019 Nov;84(11):1375-1389. (PMID: 31760924) Theranostics. 2021 Apr 3;11(12):5986-6005. (PMID: 33897894) Best Pract Res Clin Haematol. 2011 Mar;24(1):59-64. (PMID: 21396593) Front Physiol. 2016 Feb 09;7:24. (PMID: 26903875) Exp Neurol. 2020 Aug;330:113359. (PMID: 32428505) Cell Mol Life Sci. 2016 Feb;73(3):567-87. (PMID: 26514730) J Physiol. 2010 Oct 15;588(Pt 20):3901-20. (PMID: 20736421) Int J Mol Sci. 2018 Feb 25;19(3):. (PMID: 29495345) Stroke. 2011 Aug;42(8):2342-50. (PMID: 21737804) Nat Genet. 2000 May;25(1):25-9. (PMID: 10802651) Front Immunol. 2019 Mar 12;10:446. (PMID: 30915084) Mol Neurobiol. 2015 Feb;51(1):155-67. (PMID: 24826917) Neural Regen Res. 2020 Aug;15(8):1483-1489. (PMID: 31997812) Front Neurosci. 2021 Feb 26;15:641157. (PMID: 33716657) Glia. 2018 Jun;66(6):1185-1199. (PMID: 29274121) Bull Exp Biol Med. 2009 Feb;147(2):269-72. (PMID: 19513437) Front Cell Neurosci. 2019 Jan 10;12:526. (PMID: 30687015) J Cell Biol. 2013 Feb 18;200(4):373-83. (PMID: 23420871) Proteomics. 2019 Jan;19(1-2):e1800163. (PMID: 30467989) Hepat Med. 2018 Sep 12;10:105-115. (PMID: 30254497) Neurosignals. 2005;14(5):207-21. (PMID: 16301836) Cell Mol Life Sci. 2014 Aug;71(15):2787-814. (PMID: 24442513) N Engl J Med. 1978 Oct 26;299(17):920-5. (PMID: 692597) Biochem Soc Trans. 2006 Apr;34(Pt 2):232-7. (PMID: 16545083) Neurosci Lett. 2014 Jun 13;571:55-60. (PMID: 24796809) Curr Neuropharmacol. 2020;18(7):596-612. (PMID: 31934841) Front Genet. 2020 Mar 19;11:172. (PMID: 32265978) Nucleic Acids Res. 2021 Jan 8;49(D1):D325-D334. (PMID: 33290552) Physiol Rev. 2002 Oct;82(4):893-922. (PMID: 12270947) Methods Mol Biol. 2015;1254:197-210. (PMID: 25431067) Brain Res Bull. 2018 Oct;143:155-170. (PMID: 30449731) Proc Natl Acad Sci U S A. 2014 Oct 14;111(41):14888-93. (PMID: 25267620) Antioxid Redox Signal. 2011 Apr 1;14(7):1261-73. (PMID: 20626318) J Cardiovasc Pharmacol. 1988;12 Suppl 5:S69-72. (PMID: 2469881) Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289) Nat Protoc. 2016 Dec;11(12):2301-2319. (PMID: 27809316) iScience. 2019 Jun 28;16:230-241. (PMID: 31195240) Nucleic Acids Res. 2009 Jan;37(1):1-13. (PMID: 19033363) Brain Res. 1981 Apr 27;211(1):67-77. (PMID: 7225844) Ann Neurol. 1981 Feb;9(2):131-41. (PMID: 7235629) Neuroepidemiology. 2013;40(3):154-9. (PMID: 23257914) J Clin Med. 2019 Jan 24;8(2):. (PMID: 30682787) Stem Cell Res Ther. 2018 Mar 9;9(1):63. (PMID: 29523213) Basic Res Cardiol. 2020 Mar 7;115(3):26. (PMID: 32146560) Biology (Basel). 2021 Aug 03;10(8):. (PMID: 34439975) Cell Calcium. 2008 Jun;43(6):591-601. (PMID: 18054077) Front Neurosci. 2019 Mar 05;13:163. (PMID: 30890911) Front Neuroenergetics. 2013 Dec 18;5:10. (PMID: 24385963) Lancet. 2006 May 27;367(9524):1747-57. (PMID: 16731270) J Neurosci. 2020 Dec 2;40(49):9364-9371. (PMID: 33122390) Anesthesiology. 2014 Nov;121(5):1099-121. (PMID: 25211170) Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3536-E3545. (PMID: 28396435) Nat Methods. 2016 Sep;13(9):731-40. (PMID: 27348712) Eur J Pharmacol. 2000 Jul 21;400(2-3):211-9. (PMID: 10988336) Ann Neurol. 2006 Mar;59(3):447-8. (PMID: 16489618) Int J Biol Sci. 2017 Feb 6;13(2):232-244. (PMID: 28255275) Pharmacol Res. 2015 Sep;99:377-81. (PMID: 26013908) J Mol Biol. 2016 Feb 22;428(4):688-692. (PMID: 26434508) PLoS Genet. 2021 Dec 6;17(12):e1009951. (PMID: 34871319) Neuron. 1998 Jul;21(1):13-26. (PMID: 9697848) Nat Rev Mol Cell Biol. 2019 Sep;20(9):509-510. (PMID: 31324871) Front Immunol. 2014 Oct 21;5:525. (PMID: 25374572) |
فهرسة مساهمة: | Keywords: Multipotent mesenchymal stromal cells; calcium oscillations; extracellular vesicles; neonatal hypoxic-ischemic encephalopathy; neuroprotection; the phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (AKT) signaling pathway; traumatic brain injury |
المشرفين على المادة: | 0 (Proteome) 4L6452S749 (Inositol) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SY7Q814VUP (Calcium) |
تواريخ الأحداث: | Date Created: 20220923 Date Completed: 20220926 Latest Revision: 20221012 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC9461662 |
DOI: | 10.7150/ijbs.73747 |
PMID: | 36147480 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1449-2288 |
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DOI: | 10.7150/ijbs.73747 |