دورية أكاديمية
IL27 gene expression distinguishes multisystem inflammatory syndrome in children from febrile illness in a South African cohort.
| العنوان: | IL27 gene expression distinguishes multisystem inflammatory syndrome in children from febrile illness in a South African cohort. |
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| المؤلفون: | Spracklen TF; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Cape Heart Institute, University of Cape Town, Cape Town, South Africa., Mendelsohn SC; South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa., Butters C; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Division of Immunology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa., Facey-Thomas H; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa., Stander R; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa., Abrahams D; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa., Erasmus M; South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa., Baguma R; South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa., Day J; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa., Scott C; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa., Zühlke LJ; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Cape Heart Institute, University of Cape Town, Cape Town, South Africa.; South African Medical Research Council, Cape Town, South Africa., Kassiotis G; Retroviral Immunology Laboratory, The Francis Crick Institute, London, United Kingdom.; Department of Infectious Disease, St Mary's Hospital, Imperial College, London, United Kingdom., Scriba TJ; South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa., Webb K; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Crick African Network, The Francis Crick Institute, London, United Kingdom. |
| المصدر: | Frontiers in immunology [Front Immunol] 2022 Sep 06; Vol. 13, pp. 992022. Date of Electronic Publication: 2022 Sep 06 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | COVID-19*/complications , COVID-19*/genetics , Interleukin-27*, Antiviral Agents ; Child ; Cytokines ; Gene Expression ; Humans ; Interleukin-1 ; SARS-CoV-2 ; South Africa ; Systemic Inflammatory Response Syndrome |
| مستخلص: | Introduction: Multisystem inflammatory syndrome in children (MIS-C) is a severe acute inflammatory reaction to SARS-CoV-2 infection in children. There is a lack of data describing differential expression of immune genes in MIS-C compared to healthy children or those with other inflammatory conditions and how expression changes over time. In this study, we investigated expression of immune-related genes in South African MIS-C patients and controls. Methods: The cohort included 30 pre-treatment MIS-C cases and 54 healthy non-inflammatory paediatric controls. Other controls included 34 patients with juvenile systemic lupus erythematosus, Kawasaki disease or other inflammatory conditions. Longitudinal post-treatment MIS-C specimens were available at various timepoints. Expression of 80 immune-related genes was determined by real-time quantitative PCR. Results: A total of 29 differentially expressed genes were identified in pre-treatment MIS-C compared to healthy controls. Up-regulated genes were found to be overrepresented in innate immune pathways including interleukin-1 processing and pyroptosis. Post-treatment follow-up data were available for up to 1,200 hours after first treatment. All down-regulated genes and 17/18 up-regulated genes resolved to normal levels in the timeframe, and all patients clinically recovered. When comparing MIS-C to other febrile conditions, only IL27 expression could differentiate these two groups with high sensitivity and specificity. Conclusions: These data indicate a unique 29-gene signature of MIS-C in South African children. The up-regulation of interleukin-1 and pyroptosis pathway genes highlights the role of the innate immune system in MIS-C. IL-27 is a potent anti-inflammatory and antiviral cytokine that may distinguish MIS-C from other conditions in our setting. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Spracklen, Mendelsohn, Butters, Facey-Thomas, Stander, Abrahams, Erasmus, Baguma, Day, Scott, Zühlke, Kassiotis, Scriba and Webb.) |
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| معلومات مُعتمدة: | D43 TW010559 United States TW FIC NIH HHS; MR/P028071/1 United Kingdom MRC_ Medical Research Council; MR/S005242/1 United Kingdom MRC_ Medical Research Council; United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: COVID-19; SARS-CoV-2; South Africa; children; multisystem inflammatory syndrome |
| المشرفين على المادة: | 0 (Antiviral Agents) 0 (Cytokines) 0 (Interleukin-1) 0 (Interleukin-27) |
| SCR Disease Name: | pediatric multisystem inflammatory disease, COVID-19 related |
| تواريخ الأحداث: | Date Created: 20220923 Date Completed: 20220926 Latest Revision: 20230308 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9486543 |
| DOI: | 10.3389/fimmu.2022.992022 |
| PMID: | 36148243 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2022.992022 |