دورية أكاديمية
Intrinsic and synaptic properties of adult mouse spinoperiaqueductal gray neurons and the influence of neonatal tissue damage.
العنوان: | Intrinsic and synaptic properties of adult mouse spinoperiaqueductal gray neurons and the influence of neonatal tissue damage. |
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المؤلفون: | Li J; Department of Anesthesiology, Pain Research Center, University of Cincinnati Medical Center, Cincinnati, OH, United States., Serafin EK, Baccei ML |
المصدر: | Pain [Pain] 2023 Apr 01; Vol. 164 (4), pp. 905-917. Date of Electronic Publication: 2022 Sep 23. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 7508686 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6623 (Electronic) Linking ISSN: 03043959 NLM ISO Abbreviation: Pain Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2015- : Hagerstown, MD : Lippincott Williams & Wilkins Original Publication: Amsterdam, Elsevier/North-Holland. |
مواضيع طبية MeSH: | Spinal Cord Dorsal Horn* , Interneurons*, Animals ; Mice ; Female ; Male ; Animals, Newborn ; Membrane Potentials/physiology ; Nerve Fibers, Unmyelinated ; Spinal Cord |
مستخلص: | Abstract: The periaqueductal gray (PAG) represents a key target of projection neurons residing in the spinal dorsal horn. In comparison to lamina I spinoparabrachial neurons, little is known about the intrinsic and synaptic properties governing the firing of spino-PAG neurons, or whether such activity is modulated by neonatal injury. In this study, this issue was addressed using ex vivo whole-cell patch clamp recordings from lamina I spino-PAG neurons in adult male and female FVB mice after hindpaw incision at postnatal day (P)3. Spino-PAG neurons were classified as high output, medium output, or low output based on their action potential discharge after dorsal root stimulation. The high-output subgroup exhibited prevalent spontaneous burst firing and displayed initial burst or tonic patterns of intrinsic firing, whereas low-output neurons showed little spontaneous activity. Interestingly, the level of dorsal root-evoked firing significantly correlated with the resting potential and membrane resistance but not with the strength of primary afferent-mediated glutamatergic drive. Neonatal incision failed to alter the pattern of monosynaptic sensory input, with most spino-PAG neurons receiving direct connections from low-threshold C-fibers. Furthermore, primary afferent-evoked glutamatergic input and action potential discharge in adult spino-PAG neurons were unaltered by neonatal surgical injury. Finally, Hebbian long-term potentiation at sensory synapses, which significantly increased afferent-evoked firing, was similar between P3-incised and naive littermates. Collectively, these data suggest that the functional response of lamina I spino-PAG neurons to sensory input is largely governed by their intrinsic membrane properties and appears resistant to the persistent influence of neonatal tissue damage. (Copyright © 2022 International Association for the Study of Pain.) |
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معلومات مُعتمدة: | R01 NS072202 United States NS NINDS NIH HHS; R01 NS080889 United States NS NINDS NIH HHS |
تواريخ الأحداث: | Date Created: 20220923 Date Completed: 20230322 Latest Revision: 20240402 |
رمز التحديث: | 20240402 |
مُعرف محوري في PubMed: | PMC10033328 |
DOI: | 10.1097/j.pain.0000000000002787 |
PMID: | 36149785 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1872-6623 |
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DOI: | 10.1097/j.pain.0000000000002787 |