دورية أكاديمية
أعرض في EDS

Intrinsic and synaptic properties of adult mouse spinoperiaqueductal gray neurons and the influence of neonatal tissue damage.

التفاصيل البيبلوغرافية
العنوان: Intrinsic and synaptic properties of adult mouse spinoperiaqueductal gray neurons and the influence of neonatal tissue damage.
المؤلفون: Li J; Department of Anesthesiology, Pain Research Center, University of Cincinnati Medical Center, Cincinnati, OH, United States., Serafin EK, Baccei ML
المصدر: Pain [Pain] 2023 Apr 01; Vol. 164 (4), pp. 905-917. Date of Electronic Publication: 2022 Sep 23.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 7508686 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6623 (Electronic) Linking ISSN: 03043959 NLM ISO Abbreviation: Pain Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: Amsterdam, Elsevier/North-Holland.
مواضيع طبية MeSH: Spinal Cord Dorsal Horn* , Interneurons*, Animals ; Mice ; Female ; Male ; Animals, Newborn ; Membrane Potentials/physiology ; Nerve Fibers, Unmyelinated ; Spinal Cord
مستخلص: Abstract: The periaqueductal gray (PAG) represents a key target of projection neurons residing in the spinal dorsal horn. In comparison to lamina I spinoparabrachial neurons, little is known about the intrinsic and synaptic properties governing the firing of spino-PAG neurons, or whether such activity is modulated by neonatal injury. In this study, this issue was addressed using ex vivo whole-cell patch clamp recordings from lamina I spino-PAG neurons in adult male and female FVB mice after hindpaw incision at postnatal day (P)3. Spino-PAG neurons were classified as high output, medium output, or low output based on their action potential discharge after dorsal root stimulation. The high-output subgroup exhibited prevalent spontaneous burst firing and displayed initial burst or tonic patterns of intrinsic firing, whereas low-output neurons showed little spontaneous activity. Interestingly, the level of dorsal root-evoked firing significantly correlated with the resting potential and membrane resistance but not with the strength of primary afferent-mediated glutamatergic drive. Neonatal incision failed to alter the pattern of monosynaptic sensory input, with most spino-PAG neurons receiving direct connections from low-threshold C-fibers. Furthermore, primary afferent-evoked glutamatergic input and action potential discharge in adult spino-PAG neurons were unaltered by neonatal surgical injury. Finally, Hebbian long-term potentiation at sensory synapses, which significantly increased afferent-evoked firing, was similar between P3-incised and naive littermates. Collectively, these data suggest that the functional response of lamina I spino-PAG neurons to sensory input is largely governed by their intrinsic membrane properties and appears resistant to the persistent influence of neonatal tissue damage.
(Copyright © 2022 International Association for the Study of Pain.)
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معلومات مُعتمدة: R01 NS072202 United States NS NINDS NIH HHS; R01 NS080889 United States NS NINDS NIH HHS
تواريخ الأحداث: Date Created: 20220923 Date Completed: 20230322 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC10033328
DOI: 10.1097/j.pain.0000000000002787
PMID: 36149785
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-6623
DOI:10.1097/j.pain.0000000000002787