دورية أكاديمية
أعرض في EDS

Single-cell mapping of regenerative and fibrotic healing responses after musculoskeletal injury.

التفاصيل البيبلوغرافية
العنوان: Single-cell mapping of regenerative and fibrotic healing responses after musculoskeletal injury.
المؤلفون: Tower RJ; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: robert.tower@utsouthwestern.edu., Bancroft AC; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Chowdary AR; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Barnes S; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Bioinformatics Core, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Edwards NJ; Department of Surgery, University of Michigan, Ann Arbor, MI 48109, USA., Pagani CA; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Dawson LA; Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX 77843, USA., Levi B; Center for Organogenesis and Trauma, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: benjamin.levi@utsouthwestern.edu.
المصدر: Stem cell reports [Stem Cell Reports] 2022 Oct 11; Vol. 17 (10), pp. 2334-2348. Date of Electronic Publication: 2022 Sep 22.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101611300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-6711 (Electronic) Linking ISSN: 22136711 NLM ISO Abbreviation: Stem Cell Reports Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2013-
مواضيع طبية MeSH: Bone and Bones*/injuries , Musculoskeletal System*/injuries , Ossification, Heterotopic* , Regeneration*, Amputation, Surgical ; Cell Differentiation ; Humans ; Transforming Growth Factor beta
مستخلص: After injury, a cascade of events repairs the damaged tissue, including expansion and differentiation of the progenitor pool and redeposition of matrix. To guide future wound regeneration strategies, we compared single-cell sequencing of regenerative (third phalangeal element [P3]) and fibrotic (second phalangeal element [P2]) digit tip amputation (DTA) models as well as traumatic heterotopic ossification (HO; aberrant). Analyses point to a common initial response to injury, including expansion of progenitors, redeposition of matrix, and activation of transforming growth factor β (TGF-β) and WNT pathways. Surprisingly, fibrotic P2 DTA showed greater transcriptional similarity to HO than to regenerative P3 DTA, suggesting that gene expression more strongly correlates with healing outcome than with injury type or cell origin. Differential analysis and immunostaining revealed altered activation of inflammatory pathways, such as the complement pathway, in the progenitor cells. These data suggests that common pathways are activated in response to damage but are fine tuned within each injury. Modulating these pathways may shift the balance toward regenerative outcomes.
Competing Interests: Conflict of interests The authors declare no competing interests.
(Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R01 AR071379 United States AR NIAMS NIH HHS; R01 AR078324 United States AR NIAMS NIH HHS; T32 HD007505 United States HD NICHD NIH HHS
فهرسة مساهمة: Keywords: digit tip amputation; fibrosis; heterotopic ossification; injury repair; mesenchymal progenitor cells; regeneration
المشرفين على المادة: 0 (Transforming Growth Factor beta)
تواريخ الأحداث: Date Created: 20220923 Date Completed: 20221014 Latest Revision: 20230701
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9561541
DOI: 10.1016/j.stemcr.2022.08.011
PMID: 36150381
قاعدة البيانات: MEDLINE
الوصف
تدمد:2213-6711
DOI:10.1016/j.stemcr.2022.08.011