دورية أكاديمية
Profiles of transcriptome and metabolic pathways after hypobaric hypoxia exposure.
| العنوان: | Profiles of transcriptome and metabolic pathways after hypobaric hypoxia exposure. |
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| المؤلفون: | Xu J; Qinghai University, Xining, 810001, China., Chen WJ; Qinghai University Clinical Medicine Class 6, Grade 20, Xining, 810001, China., Wang Z; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China., Xin MY; Qinghai University Clinical Medicine Class 1, Grade 19, Xining, 810001, China., Gao SH; Qinghai University, Plateau Biology Jing Ying Class, 19 Grade, Xining, 810001, China., Liu WJ; Qinghai University the Graduate Student of Foundation Medical of 2020, Xining, 810001, China., Wang KK; Qinghai University Clinical Medicine Class 3, Grade 20, Xining, 810001, China., Ma JW; Qinghai University Clinical Medicine Class 1, Grade 19, Xining, 810001, China., Yan XZ; Qinghai University the Graduate Student of Foundation Medical of 2021, Xining, 810001, China., Ren YM; Qinghai University, Xining, 810001, China. QH202120212021@163.com. |
| المصدر: | Proteome science [Proteome Sci] 2022 Sep 24; Vol. 20 (1), pp. 16. Date of Electronic Publication: 2022 Sep 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101170539 Publication Model: Electronic Cited Medium: Print ISSN: 1477-5956 (Print) Linking ISSN: 14775956 NLM ISO Abbreviation: Proteome Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : BioMed Central, c2003- |
| مستخلص: | Background: Hypoxia is a risk factor for non-alcoholic fatty liver diseases, leading to permanent imbalance of liver lipid homeostasis and steatohepatitis. However, a detailed understanding of the metabolic genes and pathways involved remains elusive. Methods: In vivo experiments were designed to analyze body weight and lipid metabolism changes of rats under hypoxia. After this, we combined microarray analysis and gene overexpression experiments to validate the core mechanisms involved in the response to hypoxia. Results: The hypobaric hypoxia treated rats exhibited significantly increased serum triglycerides (TG) (p < 0.05), despite no significant changes in serum alanine aminotransferase (ALT) and blood glucose (BG) were observed. In addition, serum high-density lipoprotein cholesterol (HDL-C) greatly increased after 3 days and then returned to normal level at 30 days. Interestingly, serum low-density lipoprotein cholesterol (LDL-C) showed an opposite pattern. Transcriptome analysis, qRT-PCR, ICC revealed that the genes PPARA, ANGPTL4, CPT-I, ACC and LPL play a crucial role in response to hypobaric hypoxia. IPA pathway analysis further confirmed that PPARA-mediated regulation of ANGPTL4 participated in TG clearance and lipoprotein metabolism. Finally, the PPARA-ANGPTL4 pathway was validated in rats and HL 7702 cells treated with Fenofibrate, a PPARA specific agonist. Conclusions: Our study showed this pathway plays an important role on lipid metabolism caused by hypobaric hypoxia and the potential target genes associated with oxygen-dependent lipid homeostasis in the liver. (© 2022. The Author(s).) |
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| فهرسة مساهمة: | Keywords: ANGPTL4; Hypobaric hypoxia; Lipid metabolism; Microarray; PPARA |
| تواريخ الأحداث: | Date Created: 20220924 Latest Revision: 20240904 |
| رمز التحديث: | 20240904 |
| مُعرف محوري في PubMed: | PMC9508752 |
| DOI: | 10.1186/s12953-022-00198-y |
| PMID: | 36153524 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1477-5956 |
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| DOI: | 10.1186/s12953-022-00198-y |