دورية أكاديمية
Interaction between Benzo[a]anthracene 7,2-dione 7-oxime (BZA) and calf thymus dsDNA using electroanalytical genosensor.
| العنوان: | Interaction between Benzo[a]anthracene 7,2-dione 7-oxime (BZA) and calf thymus dsDNA using electroanalytical genosensor. |
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| المؤلفون: | Pinheiro-Sousa DB; Coordenação do Curso de Engenharia Ambiental, Universidade Federal do Maranhão, CEP 65800-000, Balsas, MA, Brazil; Programa de Pós-Graduação em Biodiversidade e Biotecnologia (REDE BIONORTE), Universidade Estadual do Maranhão, CEP 65055-310 São Luís, MA, Brazil. Electronic address: debora.batista@ufma.br., Sousa Lima MI; Departamento de Biologia, Universidade Federal do Maranhão, Campus Paulo VI, CEP 65055-970, São Luis, MA, Brazil., Gonçalves RM; Departamento de Biologia, Universidade Federal do Maranhão, Campus Paulo VI, CEP 65055-970, São Luis, MA, Brazil., Silva Santos DM; Departamento de Biologia, Universidade Estadual do Maranhão, CEP 65080-805 São Luís, MA, Brazil., Vieira de Carvalho Neta A; Departamento de Biologia, Universidade Estadual do Maranhão, CEP 65080-805 São Luís, MA, Brazil., Benjamim LDA; Departamento de Medicina Veterinária, Universidade Federal de Viçosa, CEP, 36570-000, Viçosa, MG, Brazil., Nunes GS; Departamento de Tecnologia Química, Universidade Federal do Maranhão, CEP 65080-805 São Luís, MA, Brazil., Brasil de Oliveira Marques PR; Coordenação do Curso de Licenciaturas em Ciências Naturais (Biologia), Universidade Federal do Maranhão, CEP 65400-000, Codó, MA, Brazil., Fortes Carvalho-Neta RN; Departamento de Biologia, Universidade Estadual do Maranhão, CEP 65080-805 São Luís, MA, Brazil. |
| المصدر: | Analytical biochemistry [Anal Biochem] 2022 Nov 15; Vol. 657, pp. 114905. Date of Electronic Publication: 2022 Sep 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : San Diego, CA : Elsevier Original Publication: Orlando Fl : Academic Press |
| مواضيع طبية MeSH: | Biosensing Techniques*/methods , Oximes*, Anthracenes ; Carbon/chemistry ; DNA/chemistry ; Electrodes ; Guanine ; Reproducibility of Results |
| مستخلص: | In the present study, the objective was to evaluate in situ interaction between Benzo[a]anthracene 7,2-dione 7-oxime (BZA) and calf thymus dsDNA (ct-dsDNA) using electroanalytical genosensor. Analytical techniques based on Ultraviolet/Visible (UV-Vis) spectroscopy and electroanalytical were used to investigate the interaction processes in solution and immobilized on carbon screen-printed electrodes modified with electrochemical mediator Meldola blue. In addition, was possible to evaluate the degree of damage caused to the genetic material by the analyte through of toxicity estimate (S%). The interaction evaluated by genosensor showed processes of intercalation, degradation, and breaks of the double strand of ct-dsDNA, suggesting that the interaction simulates highly toxic (values varying from 0.6 to 0.8 μA in 48 h of interaction), such as 8-oxoguanine (+0.48 V), which is a by-product of guanine oxidation. Furthermore, monitoring A (+1.10 V) after 1 h showed an S% value between 50 and 90%, indicative of high toxicity, and monitoring G (+0.85 V), which showed S>90%, indicated no toxicity after 10 min. Overall, the electroanalytical genosensor developed in a miniaturized system displayed good reproducibility and stability over time being a quick alternative for assesses the degree of toxicity between toxic xenobiotics and biologically electroactive molecules, such as DNA. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 8-Oxoguanine; Chronoamperometry; Deoxyribonucleic acid; Oxidative DNA damage; Screen-printed electrodes |
| المشرفين على المادة: | 0 (Anthracenes) 0 (Oximes) 5Z93L87A1R (Guanine) 7440-44-0 (Carbon) 9007-49-2 (DNA) EH46A1TLD7 (anthracene) |
| تواريخ الأحداث: | Date Created: 20220926 Date Completed: 20221006 Latest Revision: 20221018 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.ab.2022.114905 |
| PMID: | 36154836 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0309 |
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| DOI: | 10.1016/j.ab.2022.114905 |