دورية أكاديمية
Non-Invasive Diagnostics of Renal Cell Carcinoma Using Ultrasensitive Immunodetection of Cancer-Retina Antigens.
| العنوان: | Non-Invasive Diagnostics of Renal Cell Carcinoma Using Ultrasensitive Immunodetection of Cancer-Retina Antigens. |
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| المؤلفون: | Shlyapnikov YM; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. yuri.shlyapnikov@gmail.com., Malakhova EA; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. mallakhovka@yandex.ru., Potoldykova NV; Institute for Urology and Reproductive Health, Sechenov First Moscow State Medical University, Moscow, 119991, Russia. natalis8282@mail.ru., Svetocheva YA; Institute for Urology and Reproductive Health, Sechenov First Moscow State Medical University, Moscow, 119991, Russia. svetochevaya@yandex.ru., Vinarov AZ; Institute for Urology and Reproductive Health, Sechenov First Moscow State Medical University, Moscow, 119991, Russia. avinarov@mail.ru., Zinchenko DV; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, 117997, Russia. zdv@bibch.ru., Zernii EY; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. zerni@belozersky.msu.ru., Zamyatnin AA Jr; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. zamyat@belozersky.msu.ru.; Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, 119991, Russia.; Department of Biotechnology, Sirius University of Science and Technology, Sochi, 354340, Russia.; Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK., Shlyapnikova EA; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. shlyapnikova@rambler.ru. |
| المصدر: | Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2022 Jul; Vol. 87 (7), pp. 658-666. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MAIK Nauka/Interperiodica Country of Publication: United States NLM ID: 0376536 Publication Model: Print Cited Medium: Internet ISSN: 1608-3040 (Electronic) Linking ISSN: 00062979 NLM ISO Abbreviation: Biochemistry (Mosc) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2007->: Moscow : MAIK Nauka/Interperiodica Original Publication: New York, Consultants Bureau [etc.] |
| مواضيع طبية MeSH: | Carcinoma, Renal Cell*/diagnosis , Carcinoma, Renal Cell*/pathology , Kidney Neoplasms*/diagnosis , Kidney Neoplasms*/pathology, Arrestins ; Biomarkers, Tumor ; G-Protein-Coupled Receptor Kinase 1 ; Humans ; Recoverin ; Retina ; Transducin |
| مستخلص: | Renal cell carcinoma (RCC) is the most common urological malignancy with a high mortality and low detection rate. One of the approaches to improving its diagnostics may be the search for new non-invasive biomarkers in liquid biopsy and development of more sensitive methods for their detection. Cancer-retina antigens, which are known to be aberrantly expressed in malignant tumors, are present in liquid biopsy at extremely low concentrations. Using the developed multiplex immunoassay with a detection limit of 0.1 pg/ml, urine and serum samples of 89 patients with RCC and 50 non-cancer patients were examined for the presence of cancer-retina antigens (arrestin, recoverin, rhodopsin kinase, and transducin); the difference between the RCC and control groups was evaluated with the χ 2 test. The results showed high diagnostic efficiency of a combination of arrestin and recoverin: at a threshold of 0.1 pg/ml, the sensitivity was 96%, specificity 92%, and AUC = 0.96 (95% confidence interval, 0.93-0.99). Seven days after nephrectomy, the concentration of the antigens returned to the level characteristic of the control group. Therefore, arrestin in a combination with recoverin can serve as a diagnostic non-invasive urinary biomarker of RCC. |
| فهرسة مساهمة: | Keywords: cancer-retina antigens; immunoassay; magnetic particles; renal cell carcinoma |
| المشرفين على المادة: | 0 (Arrestins) 0 (Biomarkers, Tumor) 135844-11-0 (Recoverin) EC 2.7.11.14 (G-Protein-Coupled Receptor Kinase 1) EC 3.6.5.1 (Transducin) |
| تواريخ الأحداث: | Date Created: 20220926 Date Completed: 20220928 Latest Revision: 20220928 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1134/S0006297922070070 |
| PMID: | 36154884 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1608-3040 |
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| DOI: | 10.1134/S0006297922070070 |