دورية أكاديمية
Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS.
| العنوان: | Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS. |
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| المؤلفون: | Teixeira DO; Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, CE, Brasil., Silva HVR; Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, CE, Brasil., Brito BF; Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, CE, Brasil., Barbosa BS; Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, CE, Brasil., Tabosa BEA; Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, CE, Brasil., da Silva LDM; Universidade Estadual do Ceará, Faculdade de Veterinária, Fortaleza, CE, Brasil. |
| المصدر: | Animal reproduction [Anim Reprod] 2022 Sep 05; Vol. 19 (3), pp. e20210069. Date of Electronic Publication: 2022 Sep 05 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Colégio Brasileiro de Reprodução Animal Country of Publication: Brazil NLM ID: 101272804 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-3143 (Electronic) Linking ISSN: 18069614 NLM ISO Abbreviation: Anim Reprod Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Belo Horizonte, MG, Brazil : Colégio Brasileiro de Reprodução Animal, [2004]- |
| مستخلص: | Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs . 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare. |
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| فهرسة مساهمة: | Keywords: cryodamage; cryopreservation; extenders; morphology; spermatozoa measurement |
| تواريخ الأحداث: | Date Created: 20220926 Latest Revision: 20220928 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC9484261 |
| DOI: | 10.1590/1984-3143-AR2021-0069 |
| PMID: | 36156885 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1984-3143 |
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| DOI: | 10.1590/1984-3143-AR2021-0069 |