دورية أكاديمية
أعرض في EDS

T cell interaction with activated endothelial cells primes for tissue-residency.

التفاصيل البيبلوغرافية
العنوان: T cell interaction with activated endothelial cells primes for tissue-residency.
المؤلفون: Wienke J; Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands., Veldkamp SR; Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands., Struijf EM; Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands., Yousef Yengej FA; Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands., van der Wal MM; Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands., van Royen-Kerkhof A; Pediatric Rheumatology and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, Netherlands., van Wijk F; Center for Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands.
المصدر: Frontiers in immunology [Front Immunol] 2022 Sep 12; Vol. 13, pp. 827786. Date of Electronic Publication: 2022 Sep 12 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Immunologic Memory*, CD8-Positive T-Lymphocytes/metabolism ; Cell Communication ; Cytokines/metabolism ; Endothelial Cells/metabolism ; Humans ; Integrin alpha1/metabolism ; Intercellular Adhesion Molecule-1/metabolism ; Interleukin-15/metabolism ; Ki-67 Antigen/metabolism ; Programmed Cell Death 1 Receptor/metabolism ; Receptors, Antigen, T-Cell/metabolism ; Transcription Factors/metabolism ; Vascular Cell Adhesion Molecule-1/metabolism
مستخلص: Tissue-resident memory T cells (TRM) are suspected drivers of chronic inflammation, but their induction remains unclear. Since endothelial cells (EC) are obligate interaction partners for T cells trafficking into inflamed tissues, they may play a role in TRM development. Here, we used an in vitro co-culture system of human cytokine-activated EC and FACS-sorted T cells to study the effect of EC on T(RM) cell differentiation. T cell phenotypes were assessed by flow cytometry, including proliferation measured by CellTrace Violet dilution assay. Soluble mediators were analyzed by multiplex immunoassay. Co-culture of T cells with cytokine-activated, but not resting EC induced CD69 expression without activation (CD25, Ki67) or proliferation. The dynamic of CD69 expression induced by EC was distinct from that induced by TCR triggering, with rapid induction and stable expression over 7 days. CD69 induction by activated EC was higher in memory than naive T cells, and most pronounced in CD8 + effector memory T cells. Early CD69 induction was mostly mediated by IL-15, whereas later effects were also mediated by interactions with ICAM-1 and/or VCAM-1. CD69 + T cells displayed a phenotype associated with tissue-residency, with increased CD49a, CD103, CXCR6, PD-1 and CD57 expression, and decreased CD62L and S1PR1. EC-induced CD69 + T cells were poised for high production of pro-inflammatory cytokines and showed increased expression of T-helper 1 transcription factor T-bet. Our findings demonstrate that activated EC can induce functional specialization in T cells with sustained CD69 expression, increased cytokine response and a phenotypic profile reminiscent of TRM. Interaction with activated EC during transmigration into (inflamed) tissues thus contributes to TRM-residency priming.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Wienke, Veldkamp, Struijf, Yousef Yengej, van der Wal, van Royen-Kerkhof and van Wijk.)
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فهرسة مساهمة: Keywords: CD69; T cell differentiation; endothelial cell; inflammation; tissue-resident memory T cells
المشرفين على المادة: 0 (Cytokines)
0 (Integrin alpha1)
0 (Interleukin-15)
0 (Ki-67 Antigen)
0 (Programmed Cell Death 1 Receptor)
0 (Receptors, Antigen, T-Cell)
0 (Transcription Factors)
0 (Vascular Cell Adhesion Molecule-1)
126547-89-5 (Intercellular Adhesion Molecule-1)
تواريخ الأحداث: Date Created: 20220929 Date Completed: 20220930 Latest Revision: 20221012
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9510578
DOI: 10.3389/fimmu.2022.827786
PMID: 36172363
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-3224
DOI:10.3389/fimmu.2022.827786