دورية أكاديمية
Bioanalytical method for simultaneous determination of benzodiazepines in vitreous humor using liquid chromatography-tandem mass spectrometry.
| العنوان: | Bioanalytical method for simultaneous determination of benzodiazepines in vitreous humor using liquid chromatography-tandem mass spectrometry. |
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| المؤلفون: | Birk L; Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, 90050-170, Brazil., Ossanes D; Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, 90050-170, Brazil., Silva Petry AU; Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, 90050-170, Brazil; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS, 91750-040, Brazil., Paz de Menezes F; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS, 91750-040, Brazil., Gonzaga AP; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS, 91750-040, Brazil., Schlickmann PF; Division of Postmortem Inspection, Associação Hospitalar Vila Nova, Porto Alegre, RS, 91750-040, Brazil., Eller S; Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, 90050-170, Brazil., Franco de Oliveira T; Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, 90050-170, Brazil. Electronic address: oliveira@ufcspa.edu.br. |
| المصدر: | Journal of forensic and legal medicine [J Forensic Leg Med] 2022 Oct; Vol. 91, pp. 102434. Date of Electronic Publication: 2022 Sep 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101300022 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-7487 (Electronic) Linking ISSN: 1752928X NLM ISO Abbreviation: J Forensic Leg Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kidlington, Oxford : Elsevier |
| مواضيع طبية MeSH: | Benzodiazepines*/analysis , Tandem Mass Spectrometry*/methods, Chromatography, Liquid/methods ; Diazepam/analysis ; Humans ; Reproducibility of Results ; Solvents/analysis ; Vitreous Body/chemistry |
| مستخلص: | The use of vitreous humor (VH) in forensic casework has been growing in the last years due to numerous advantages. Several compounds can be evaluated in this matrix, including benzodiazepines whose determination is essential due to their great availability and potential of dependance and misuse. Postmortem toxicological analyses are required to determine the influence of benzodiazepines in deaths. However, most of the analytical methods which determine these drugs in VH are laborious and time consuming. This article describes a simple method based on protein precipitation for the determination of eight benzodiazepines in VH samples. Samples were prepared through a protein precipitation method and analyzed by liquid chromatography tandem mass spectrometry. Solvent choice and sample and solvent volumes for precipitation were optimized using chemometric approaches. The method was validated for selectivity, lower limit of quantification (LLOQ), linearity, carryover, precision, bias, matrix effect and dilution integrity. In order to verify the applicability, 62 vitreous humor samples were analyzed. LLOQs were 1 ng/mL and calibration curves were linear from 1 to 25 ng/mL (r2 > 0,99) for all analytes. Bias, precision and dilution integrity results were satisfactory according to proper guidelines. Ionization suppression was significant with values ranging from 8 to 37%. Two samples from real cases were positive for diazepam with the following concentrations: 6.80 ng/mL and 47.68 ng/mL, approximately 10 times lower than those found in peripheral blood. The procedure described here can be used as a straightforward and low cost method for the quantitation of multiple benzodiazepines in VH. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Benzodiazepines; LC-MS/MS; Multivariate optimization; Postmortem; Protein precipitation; Vitreous humor |
| المشرفين على المادة: | 0 (Solvents) 12794-10-4 (Benzodiazepines) Q3JTX2Q7TU (Diazepam) |
| تواريخ الأحداث: | Date Created: 20221003 Date Completed: 20221011 Latest Revision: 20221011 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jflm.2022.102434 |
| PMID: | 36191450 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1878-7487 |
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| DOI: | 10.1016/j.jflm.2022.102434 |