دورية أكاديمية
أعرض في EDS

Utilization of 18s ribosomal RNA LAMP for detecting Plasmodium falciparum in microscopy and rapid diagnostic test negative patients.

التفاصيل البيبلوغرافية
العنوان: Utilization of 18s ribosomal RNA LAMP for detecting Plasmodium falciparum in microscopy and rapid diagnostic test negative patients.
المؤلفون: Aninagyei E; Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Volta Region, Ghana., Boakye AA; Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Volta Region, Ghana., Tettey CO; Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Volta Region, Ghana., Ntiri KA; Ghana Health Service, Greater Accra Region, Mayera-Faase Health Centre, Mayera-Faase, Ghana., Ofori SO; Medical Laboratory Department, Accra Technical University, Accra, Ghana., Tetteh CD; Ghana Health Service, Municipal Health Directorate, Ga West Municipal, Amasaman, Ghana., Aphour TT; Ghana Health Service, Greater Accra Region, Ablekuma North Health Directorate, Accra, Ghana., Rufai T; Ghana Health Service, Eastern Region, New Juaben South Municipal Health Directorate, Koforidua, Ghana.; Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States of America.
المصدر: PloS one [PLoS One] 2022 Oct 06; Vol. 17 (10), pp. e0275052. Date of Electronic Publication: 2022 Oct 06 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Malaria*/diagnosis , Malaria, Falciparum*/diagnosis , Plasmodium*/genetics, Child ; Diagnostic Tests, Routine/methods ; Female ; Humans ; Male ; Microscopy ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques/methods ; Plasmodium falciparum/genetics ; RNA, Ribosomal, 18S/genetics ; Reagent Kits, Diagnostic ; Sensitivity and Specificity
مستخلص: In this study, Plasmodium falciparum was detected in patients that were declared negative for malaria microscopy and rapid diagnostic test kit (mRDT), using Plasmodium 18s rRNA loop-mediated isothermal amplification (LAMP) technique. The main aim of this study was to assess the usefulness of LAMP assay for detecting pre-clinical malaria, when microscopy and mRDT were less sensitive. DNA was obtained from 100 μL of whole blood using the boil and spin method. Subsequently, the Plasmodium 18s rRNA LAMP assay was performed to amplify the specific Plasmodium 18s rRNA gene. Microscopy and mRDT negative samples [697/2223 (31.2%)] were used for this study. Compared to frequencies obtained for the other demographic variables, most of the patients were < 6 years (37.7%), females (59.0%), peri-urban dwellers (39.0%) and patients that sought outpatient department services (39.3%). Overall, the prevalence of Plasmodium 18s rRNA was 17.5%. when stratified by study variables, Plasmodium 18s rRNA LAMP positivity was higher in patients over 30 years [58/122 (54.2%)], males [69/122 (56.5%)], rural dwellers [69/122 (56.5%)] and patients that sought OPD services [68/122 (55.7%)]. The risk of being infected with Plasmodium when routine tests were negative was higher in 15-30-year group (OR = 3.03, 95% CI: 1.6-5.8, p = 0.0007), patients > 30 years (OR = 15.2, 95% CI: 8.3-27.7, p<0.001), males (OR = 2.1, 95% CI: 1.4-3.2, p = 0.0002) and rural dwellers (OR = 2.2, 95% CI:1.4-3.6, p = 0.0009). However, risk was lower in post-natal children (OR = 0.3, 95% CI: 0.18-0.51, p<0.001). Majority (81.5%) of the infected patients presented with headache, herpes labialis, diarrhea and vomiting. We demonstrated the lack of sensitivities of microscopy and mRDT for one-time diagnosis of malaria. Therefore, it is essential to utilize a sensitive technique such as Plasmodium 18s rRNA LAMP to increase the detection rate of Plasmodium infection.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (RNA, Ribosomal, 18S)
0 (Reagent Kits, Diagnostic)
SCR Protocol: LAMP assay
تواريخ الأحداث: Date Created: 20221006 Date Completed: 20221010 Latest Revision: 20240905
رمز التحديث: 20240905
مُعرف محوري في PubMed: PMC9536604
DOI: 10.1371/journal.pone.0275052
PMID: 36201568
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0275052