Impact of tobacco smoking in association with H2BFWT, PRM1 and PRM2 genes variants on male infertility.

التفاصيل البيبلوغرافية
العنوان:	Impact of tobacco smoking in association with H2BFWT, PRM1 and PRM2 genes variants on male infertility.
المؤلفون:	Amor H; Department of Obstetrics & Gynaecology, Saarland University, Homburg, Germany., Jankowski PM; Department of Obstetrics & Gynaecology, Saarland University, Homburg, Germany., Dahadhah FW; Department of Obstetrics & Gynaecology, Saarland University, Homburg, Germany., Al Zoubi MS; Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan., Hammadeh ME; Department of Obstetrics & Gynaecology, Saarland University, Homburg, Germany.
المصدر:	Andrologia [Andrologia] 2022 Dec; Vol. 54 (11), pp. e14611. Date of Electronic Publication: 2022 Oct 10.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Blackwell Pub Country of Publication: Germany NLM ID: 0423506 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0272 (Electronic) Linking ISSN: 03034569 NLM ISO Abbreviation: Andrologia Subsets: MEDLINE
أسماء مطبوعة:	Publication: : Berlin : Blackwell Pub. Original Publication: Berlin, Grosse
مواضيع طبية MeSH:	Infertility, Male/genetics , Infertility, Male/metabolism , Protamines/genetics , Protamines/metabolism , Semen*/metabolism, Humans ; Male ; DNA/metabolism ; Histones/metabolism ; Spermatozoa/metabolism ; Tobacco Smoking
مستخلص:	Tobacco's genotoxic components can cause a wide range of gene defects in spermatozoa such as single- or double-strand DNA breaks, cross-links, DNA-adducts, higher frequencies of aneuploidy and chromosomal abnormalities. The aim in this study was to determine the correlation between sperm quality determined by standard parameters, sperm DNA maturity tested by Chromomycin A3 (CMA3) staining, sperm DNA fragmentation tested by TUNEL assay and tobacco smoking in association with the single nucleotides polymorphisms (SNP) of three nuclear protein genes in spermatozoa (H2BFWT, PRM1 and PRM2). In this study, semen samples of 167 male patients were collected and divided into 54 non-smokers and 113 smokers. The target sequences in the extracted sperm DNA were amplified by PCR followed by Sanger sequencing. The results showed the presence of three variants: rs7885967, rs553509 and rs578953 in H2BFWT gene in the study population. Only one variant rs737008 was detected in PRM1 gene, and three variants were detected in the PRM2 gene: rs2070923, rs1646022 and rs424908. No significant association was observed between the concentration, progressive motility, morphology and the occurrence of H2BFWT, PRM1 and PRM2 SNPs. However, sperm parameters were significantly lower in heavy smokers compared to controls (p < 0.01) (sperm count: 46.00 vs. 78.50 mill/ml, progressive motility: 15.00% vs. 22.00%, and morphology 4.00% vs. 5.00%, respectively). Moreover, the heavy smoker individuals exhibited a considerable increase in CMA3 positivity and sDF compared to non-smokers (p < 0.01) (29.50% vs. 20.50% and 24.50% vs. 12.00%, respectively). In conclusion, smoking altered sperm parameters and sperm DNA integrity, but did not show a linkage with genetic variants in H2BFWT, and protamine genes (PRM1 and PRM2). (© 2022 The Authors. Andrologia published by Wiley-VCH GmbH.)
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معلومات مُعتمدة:	Universität des Saarlandes
فهرسة مساهمة:	Keywords: H2BFWT; PRM1; SNPs; male infertility; tobacco smoking
المشرفين على المادة:	9007-49-2 (DNA) 0 (Histones) 0 (PRM1 protein, human) 0 (Protamines) 0 (protamine 2)
تواريخ الأحداث:	Date Created: 20221011 Date Completed: 20221205 Latest Revision: 20221205
رمز التحديث:	20221206
DOI:	10.1111/and.14611
PMID:	36217675
قاعدة البيانات:	MEDLINE

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تدمد:	1439-0272
DOI:	10.1111/and.14611