دورية أكاديمية
β-heavy-spectrin stabilizes the constricting contractile ring during cytokinesis.
العنوان: | β-heavy-spectrin stabilizes the constricting contractile ring during cytokinesis. |
---|---|
المؤلفون: | Silva AM; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal., Chan FY; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal., Norman MJ; Department of Physics, North Carolina State University, Raleigh, NC.; Quantitative and Computational Developmental Biology Cluster, North Carolina State University, Raleigh, NC., Sobral AF; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal., Zanin E; Department Biologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany., Gassmann R; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal., Belmonte JM; Department of Physics, North Carolina State University, Raleigh, NC.; Quantitative and Computational Developmental Biology Cluster, North Carolina State University, Raleigh, NC., Carvalho AX; i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal. |
المصدر: | The Journal of cell biology [J Cell Biol] 2023 Jan 02; Vol. 222 (1). Date of Electronic Publication: 2022 Oct 11. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: New York : Rockefeller University Press |
مواضيع طبية MeSH: | Actin Cytoskeleton* , Cytokinesis* , Spectrin*/metabolism, Actins ; Actomyosin ; Animals ; Caenorhabditis elegans/cytology ; Caenorhabditis elegans Proteins/metabolism ; Formins ; Membrane Glycoproteins/metabolism ; Membrane Proteins/metabolism ; Microfilament Proteins/metabolism |
مستخلص: | Cytokinesis requires the constriction of an actomyosin-based contractile ring and involves multiple F-actin crosslinkers. We show that partial depletion of the C. elegans cytokinetic formin generates contractile rings with low F-actin levels that constrict but are structurally fragile, and we use this background to investigate the roles of the crosslinkers plastin/PLST-1 and β-heavy-spectrin/SMA-1 during ring constriction. We show that the removal of PLST-1 or SMA-1 has opposite effects on the structural integrity of fragile rings. PLST-1 loss reduces cortical tension that resists ring constriction and makes fragile rings less prone to ruptures and regressions, whereas SMA-1 loss exacerbates structural defects, leading to frequent ruptures and cytokinesis failure. Fragile rings without SMA-1 or containing a shorter SMA-1, repeatedly rupture at the same site, and SMA-1::GFP accumulates at repair sites in fragile rings and in rings cut by laser microsurgery. These results establish that β-heavy-spectrin stabilizes the constricting ring and reveals the importance of β-heavy-spectrin size for network connectivity at low F-actin density. (© 2022 Silva et al.) |
References: | J Cell Sci. 2013 Jan 1;126(Pt 1):1-7. (PMID: 23516326) Development. 1998 Jun;125(11):2087-98. (PMID: 9570773) Proc Natl Acad Sci U S A. 1985 Sep;82(18):6153-7. (PMID: 3862123) J Biol Chem. 2006 May 19;281(20):14383-92. (PMID: 16556604) Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18581-6. (PMID: 19846787) Cell. 2004 Mar 5;116(5):711-23. (PMID: 15006353) Curr Biol. 2003 Oct 14;13(20):1820-3. (PMID: 14561409) Mol Biol Cell. 2018 Oct 1;29(20):2433-2449. (PMID: 30091661) Plant Cell. 2005 Aug;17(8):2296-313. (PMID: 15994911) J Biol Chem. 2005 Feb 25;280(8):6986-92. (PMID: 15591319) J Cell Biol. 1987 Mar;104(3):527-36. (PMID: 2434513) Curr Biol. 2005 Apr 26;15(8):724-31. (PMID: 15854904) Nature. 2019 Jul;571(7764):E5. (PMID: 31243360) J Cell Sci. 2020 Aug 5;133(15):. (PMID: 32620698) J Cell Sci. 2013 Apr 15;126(Pt 8):1891-901. (PMID: 23444374) Nat Cell Biol. 2013 Aug;15(8):958-66. (PMID: 23851486) J Cell Biol. 2007 Jan 29;176(3):269-75. (PMID: 17261846) J Mol Biol. 2008 Dec 12;384(2):324-34. (PMID: 18835565) Elife. 2018 Jul 02;7:. (PMID: 29963981) Curr Biol. 2005 Apr 26;15(8):732-6. (PMID: 15854905) Mol Biol Cell. 2017 Mar 1;28(5):613-623. (PMID: 28057763) J Biol Chem. 2009 May 1;284(18):12533-40. (PMID: 19251693) Biochim Biophys Acta. 2014 Feb;1838(2):620-34. (PMID: 23673272) Front Cell Dev Biol. 2020 Sep 25;8:573393. (PMID: 33102479) Methods Mol Biol. 2020;2101:297-325. (PMID: 31879911) Nature. 1982 Sep 9;299(5879):126-31. (PMID: 7110333) Biophys J. 2016 Jun 7;110(11):2528-2539. (PMID: 27276270) Nat Commun. 2017 Nov 16;8(1):1530. (PMID: 29146911) J Cell Biol. 2001 May 28;153(5):947-56. (PMID: 11381081) Nat Commun. 2019 Dec 20;10(1):5803. (PMID: 31862971) Biophys Rev. 2018 Dec;10(6):1553-1569. (PMID: 30392063) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Mol Biol Cell. 2010 Sep 15;21(18):3193-204. (PMID: 20660154) Science. 2013 Jan 25;339(6118):452-6. (PMID: 23239625) Biomolecules. 2019 May 18;9(5):. (PMID: 31109067) J Biol Chem. 1981 Sep 10;256(17):9283-8. (PMID: 6894925) J Mol Biol. 2010 Nov 5;403(4):529-45. (PMID: 20869367) PLoS One. 2011;6(9):e24656. (PMID: 21935434) Curr Biol. 2002 Dec 23;12(24):2066-75. (PMID: 12498681) Nature. 1983 Jan 20;301(5897):209-14. (PMID: 6823301) Dev Cell. 2014 Jul 28;30(2):209-23. (PMID: 25073157) Mol Cell. 2005 Apr 29;18(3):273-81. (PMID: 15866170) Proc Natl Acad Sci U S A. 1981 Nov;78(11):6849-53. (PMID: 6947259) Dev Cell. 2012 Jun 12;22(6):1247-60. (PMID: 22698284) Development. 2019 Nov 12;146(21):. (PMID: 31582415) J Cell Biol. 2017 May 1;216(5):1371-1386. (PMID: 28400443) Nature. 2005 May 26;435(7041):513-8. (PMID: 15864301) Science. 2002 Jul 26;297(5581):612-5. (PMID: 12052901) J Biol Chem. 2011 Jul 29;286(30):26964-77. (PMID: 21642440) Nat Cell Biol. 2015 Feb;17(2):148-59. (PMID: 25621953) Nature. 2019 Sep;573(7773):266-270. (PMID: 31462781) Nature. 2005 Feb 3;433(7025):488-94. (PMID: 15635372) Nat Cell Biol. 2018 Jun;20(6):688-698. (PMID: 29802406) Methods Mol Biol. 2009;545:145-64. (PMID: 19475387) Curr Biol. 2013 Jul 22;23(14):1373-9. (PMID: 23850281) Science. 2004 Jul 2;305(5680):61-6. (PMID: 15166316) Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14725-30. (PMID: 15377785) Biochemistry. 2003 Jan 21;42(2):486-96. (PMID: 12525176) Curr Biol. 2021 Dec 20;31(24):5415-5428.e10. (PMID: 34666005) Mol Biol Cell. 2019 Jan 1;30(1):96-107. (PMID: 30403552) EMBO J. 1997 Jun 2;16(11):3044-56. (PMID: 9214622) J Cell Biol. 2016 Dec 19;215(6):789-799. (PMID: 27974482) J Cell Biol. 1990 Sep;111(3):1069-79. (PMID: 2391360) |
المشرفين على المادة: | 0 (Actins) 0 (Caenorhabditis elegans Proteins) 0 (Formins) 0 (Membrane Glycoproteins) 0 (Membrane Proteins) 0 (Microfilament Proteins) 0 (SMA-1 protein, C elegans) 0 (plastin) 12634-43-4 (Spectrin) 9013-26-7 (Actomyosin) |
تواريخ الأحداث: | Date Created: 20221011 Date Completed: 20221013 Latest Revision: 20230412 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9559602 |
DOI: | 10.1083/jcb.202202024 |
PMID: | 36219157 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1540-8140 |
---|---|
DOI: | 10.1083/jcb.202202024 |