دورية أكاديمية
Investigating the bone regeneration activity of PVA nanofibers scaffolds loaded with simvastatin/chitosan nanoparticles in an induced bone defect rabbit model.
| العنوان: | Investigating the bone regeneration activity of PVA nanofibers scaffolds loaded with simvastatin/chitosan nanoparticles in an induced bone defect rabbit model. |
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| المؤلفون: | Delan WK; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, Cairo 11562, Egypt., Ali IH; Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, P.O. Box 32897, Sadat City, Egypt; Department of Chemistry, School of Sciences and Engineering, The American University in Cairo (AUC), AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt., Zakaria M; Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University, Kasr Al Aini Street, Cairo, Egypt., Elsaadany B; Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University, Kasr Al Aini Street, Cairo, Egypt., Fares AR; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, Cairo 11562, Egypt., ElMeshad AN; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr el Aini Street, Cairo 11562, Egypt; Department of Pharmaceutics, Faculty of Pharmacy and Drug Technology, The Egyptian Chinese University, Gesr El Suez Street, Cairo 11786, Egypt., Mamdouh W; Department of Chemistry, School of Sciences and Engineering, The American University in Cairo (AUC), AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt. Electronic address: wael_mamdouh@aucegypt.edu. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2022 Dec 01; Vol. 222 (Pt B), pp. 2399-2413. Date of Electronic Publication: 2022 Oct 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Chitosan* , Nanofibers* , Nanoparticles*, Animals ; Rabbits ; Simvastatin/pharmacology ; Bone Regeneration ; Tissue Scaffolds |
| مستخلص: | This study aims at preparing electrospun PVA NFs incorporating simvastatin/chitosan nanoparticles (SIM CS NPs) as a controlled drug eluting scaffold for bone regeneration. Optimization was performed by Design Expert® software through establishing two factor, three level factorial design, where the independent variables were the applied voltage, flow rate and PVA solution/SIM CS NPs ratio. Formulation variables values for the optimized formula were 18KV, 0.5 mL/h, and 3:1 respectively. NFs diameter and mesh pore size were chosen as the dependent variables. The optimized NFs were evaluated morphologically, chemically, and physically. Additionally, in-vitro SIM release from the scaffolds was investigated along 24 days. Optimum NFs possessed 136 nm diameter size and 6.5 nm porosity. Also, they showed sustained SIM release for 24 days to achieve the desired goal in bone regeneration. The optimized NFs were implanted within induced bone defects in rabbits. In-vivo assessments were performed through cone beam computed tomography 3D images, bone density measurements, histological analysis and bone morphogenetic protein 2 (BMP 2) level. The obtained results proved the high potential of the optimized NFs in promoting bone regeneration compared to untreated group, non-medicated NFs group, free SIM group and NFs loaded with SIM group after 6 weeks of implantation. Competing Interests: Declaration of competing interest None. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bone regeneration; Chitosan nanoparticles; PVA NFs; Scaffold; Simvastatin |
| المشرفين على المادة: | 9012-76-4 (Chitosan) AGG2FN16EV (Simvastatin) |
| تواريخ الأحداث: | Date Created: 20221011 Date Completed: 20221111 Latest Revision: 20221111 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.ijbiomac.2022.10.026 |
| PMID: | 36220413 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2022.10.026 |