دورية أكاديمية
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Microbial Degradation, Spectral analysis and Toxicological Assessment of Malachite Green Dye by Streptomyces exfoliatus .

التفاصيل البيبلوغرافية
العنوان: Microbial Degradation, Spectral analysis and Toxicological Assessment of Malachite Green Dye by Streptomyces exfoliatus .
المؤلفون: Abu-Hussien SH; Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt., Hemdan BA; Environmental and Climate Change Research Institute, National Research Centre, Giza 1266, Egypt., Alzahrani OM; Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Alswat AS; Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Alatawi FA; Biology department, Faculty of science, Tabuk University, Tabuk 71491, Saudi Arabia., Alenezi MA; Biology department, Faculty of science, Tabuk University, Tabuk 71491, Saudi Arabia., Darwish DBE; Biology department, Faculty of science, Tabuk University, Tabuk 71491, Saudi Arabia.; Botany Department, Faculty of science, Mansoura University, Mansoura 35511, Egypt., Bafhaid HS; Pharmacology and Toxicology Department, College of Pharmacy, Umm Al Qura University, Makkah 24381, Saudi Arabia., Mahmoud SF; Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Ibrahim MFM; Department of Agricultural Botany, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt., El-Sayed SM; Department of Biochemistry, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2022 Sep 30; Vol. 27 (19). Date of Electronic Publication: 2022 Sep 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Environmental Pollutants* , Streptomyces*/genetics , Streptomyces*/metabolism, Biodegradation, Environmental ; Coloring Agents/chemistry ; Ecosystem ; Esters ; Humans ; Laccase ; Mannitol ; RNA, Ribosomal, 16S/genetics ; Rosaniline Dyes ; Silanes ; Soil ; Water
مستخلص: Malachite green (MG) dye is a common environmental pollutant that threatens human health and the integrity of the Earth's ecosystem. The aim of this study was to investigate the potential biodegradation of MG dye by actinomycetes species isolated from planted soil near an industrial water effluent in Cairo, Egypt. The Streptomyces isolate St 45 was selected according to its high efficiency for laccase production. It was identified as S. exfoliatus based on phenotype and 16S rRNA molecular analysis and was deposited in the NCBI GenBank with the gene accession number OL720220. Its growth kinetics were studied during an incubation time of 144 h, during which the growth rate was 0.4232 (µ/h), the duplication time (td) was 1.64 d, and multiplication rate (MR) was 0.61 h, with an MG decolorization value of 96% after 120 h of incubation at 25 °C. Eleven physical and nutritional factors (mannitol, frying oil waste, MgSO 4 , NH 4 NO 3 , NH 4 Cl, dye concentration, pH, agitation, temperature, inoculum size, and incubation time) were screened for significance in the biodegradation of MG by S. exfoliatus using PBD. Out of the eleven factors screened in PBD, five (dye concentration, frying oil waste, MgSO 4 , inoculum size, and pH) were shown to be significant in the decolorization process. Central composite design (CCD) was applied to optimize the biodegradation of MG. Maximum decolorization was attained using the following optimal conditions: food oil waste, 7.5 mL/L; MgSO 4 , 0.35 g/L; dye concentration, 0.04 g/L; pH, 4.0; and inoculum size, 12.5%. The products from the degradation of MG by S. exfoliatus were characterized using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The results revealed the presence of several compounds, including leuco-malachite green, di(tert-butyl)(2-phenylethoxy) silane, 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester, 1,4-benzenedicarboxylic acid, bis(2-ethylhexyl) ester, 1,2-benzenedicarboxylic acid, di-n-octyl phthalate, and 1,2-benzenedicarboxylic acid, dioctyl ester. Moreover, the phytotoxicity, microbial toxicity, and cytotoxicity tests confirmed that the byproducts of MG degradation were not toxic to plants, microbes, or human cells. The results of this work implicate S. exfoliatus as a novel strain for MG biodegradation in different environments.
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فهرسة مساهمة: Keywords: Streptomyces exfoliatus; biodegradation; cytotoxicity; decolorization; gas chromatography-mass spectrometry GC-MS; malachite green dye; phytotoxicity; response surface methodology
المشرفين على المادة: 0 (Coloring Agents)
0 (Environmental Pollutants)
0 (Esters)
0 (RNA, Ribosomal, 16S)
0 (Rosaniline Dyes)
0 (Silanes)
0 (Soil)
059QF0KO0R (Water)
12058M7ORO (malachite green)
3OWL53L36A (Mannitol)
EC 1.10.3.2 (Laccase)
SCR Organism: Streptomyces exfoliatus
تواريخ الأحداث: Date Created: 20221014 Date Completed: 20221017 Latest Revision: 20221019
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9572514
DOI: 10.3390/molecules27196456
PMID: 36234993
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules27196456