دورية أكاديمية
NKp46 + natural killer cells develop an activated/memory-like phenotype and contribute to innate immunity against experimental filarial infection.
| العنوان: | NKp46 + natural killer cells develop an activated/memory-like phenotype and contribute to innate immunity against experimental filarial infection. |
|---|---|
| المؤلفون: | Pionnier N; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; Centre for Bioscience, John Dalton Building, Faculty of Science and Engineering, Manchester Metropolitan University, Manchester, United Kingdom., Furlong-Silva J; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Colombo SAP; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Marriott AE; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Chunda VC; Parasite and Vector Biology Research Unit, Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Ndzeshang BL; Parasite and Vector Biology Research Unit, Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Sjoberg H; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Archer J; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Steven A; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Wanji S; Parasite and Vector Biology Research Unit, Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon., Taylor MJ; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Turner JD; Centre for Drugs and Diagnostics, Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom. |
| المصدر: | Frontiers in immunology [Front Immunol] 2022 Sep 27; Vol. 13, pp. 969340. Date of Electronic Publication: 2022 Sep 27 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Immunity, Innate* , Interferon-gamma*, Animals ; Antigens, Ly ; Inflammation ; Interleukin-1 Receptor-Like 1 Protein ; Killer Cells, Natural ; Male ; Mice ; Mice, Inbred BALB C ; Natural Cytotoxicity Triggering Receptor 1 ; Phenotype |
| مستخلص: | Lymphatic filariasis and onchocerciasis are major neglected tropical diseases affecting over 90 million people worldwide with painful and profoundly disfiguring pathologies (such as lymphoedema or blindness). Type 2 inflammation is a hallmark of filarial nematode tissue infection and is implicated both in eosinophil dependent immunity and lymphatic or ocular immunopathologies. Type-2 innate lymphoid cells (ILC2) are known to play an important role in the initiation of type 2 inflammation in helminth infection. We therefore tracked comparative IL-12Rβ2 + ILC1, ST2 + ILC2 and NKp46 + natural killer (NK) innate lymphoid cell population expansions during Brugia malayi experimental peritoneal filarial infections using either immunocompetent or immunodeficient mice. In immunocompetent BALB/c animals, NKp46 + NK cells rapidly expanded representing over 90% of the ILC population in the first week of infection, whereas, surprisingly, ST2 + ILC2 failed to expand. NKp46 + NK cell expansions were confirmed in RAG2 deficient mice lacking adaptive immunity. Ablation of the NKp46 + NK cell compartment in RAG2 common gamma chain (gc) mice led to increased susceptibility to chronic adult B. malayi infection. This data was recapitulated using an Onchocerca ochengi male worm peritoneal implant model. When NKp46 + NK cells were depleted in RAG2 deficient mice using anti-NKp46 or asialo GM1 antibody injections over the first five weeks of B. malayi infection, susceptibility to adult B. malayi infection was significantly increased by 2-3 fold with concomitant impairment in eosinophil or neutrophil recruitments. Finally, we demonstrate that in RAG2 deficient mice, drug clearance of a primary adult B. malayi infection followed by challenge infection leads to resistance against early larval B. malayi establishment. This innate resistance is associated with bolstered NK and eosinophils whereby NKp46 + NK cells express markers of memory-like/enhanced activation (increased expression of interferon gamma and Ly6C). Our data promotes a novel functional role for NKp46 + NK cells in immunoprotection against experimental primary and secondary filarial infection which can proceed in the absence of adaptive immune regulation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Pionnier, Furlong-Silva, Colombo, Marriott, Chunda, Ndzeshang, Sjoberg, Archer, Steven, Wanji, Taylor and Turner.) |
| References: | J Infect Dis. 2009 Jun 15;199(12):1827-37. (PMID: 19456233) Front Immunol. 2019 Jun 19;10:1414. (PMID: 31275330) Infect Immun. 1997 Jul;65(7):2846-51. (PMID: 9199458) Elife. 2018 Sep 21;7:. (PMID: 30238872) J Immunol. 1990 Apr 15;144(8):3166-73. (PMID: 2324497) Mucosal Immunol. 2019 Mar;12(2):352-362. (PMID: 30401814) Expert Rev Anti Infect Ther. 2011 May;9(5):497-501. (PMID: 21609260) Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3384-9. (PMID: 17360655) Science. 2016 Apr 22;352(6284):aaf1098. (PMID: 27102489) Front Immunol. 2019 Apr 10;10:620. (PMID: 31024526) Nat Rev Immunol. 2016 Feb;16(2):112-23. (PMID: 26806484) Nature. 2010 Apr 29;464(7293):1367-70. (PMID: 20200518) Mucosal Immunol. 2016 Nov;9(6):1407-1417. (PMID: 26883724) Sci Transl Med. 2013 Feb 27;5(174):174ra26. (PMID: 23447017) Immunity. 2014 Aug 21;41(2):283-95. (PMID: 25088770) J Immunol. 2007 Aug 15;179(4):2445-56. (PMID: 17675506) Parasit Vectors. 2014 Oct 24;7:472. (PMID: 25338621) Science. 2012 Jan 20;335(6066):344-8. (PMID: 22267813) Parasite Immunol. 2014 Aug;36(8):338-46. (PMID: 24134686) Cell. 2018 Aug 23;174(5):1054-1066. (PMID: 30142344) J Immunol. 2009 Sep 15;183(6):3906-14. (PMID: 19692641) Trans R Soc Trop Med Hyg. 2021 Mar 6;115(3):261-268. (PMID: 33515454) J Exp Med. 2013 Mar 11;210(3):535-49. (PMID: 23420878) Front Immunol. 2019 Apr 04;10:623. (PMID: 31019505) Bone Marrow Transplant. 2000 Dec;26(11):1211-6. (PMID: 11149733) PLoS Pathog. 2019 Nov 15;15(11):e1007926. (PMID: 31730667) J Exp Med. 1999 Feb 1;189(3):553-62. (PMID: 9927517) PLoS Pathog. 2018 Mar 16;14(3):e1006949. (PMID: 29547639) J Biol Chem. 2009 Aug 14;284(33):22364-22378. (PMID: 19458089) Front Immunol. 2017 Sep 13;8:1143. (PMID: 28955346) PLoS One. 2014 Apr 11;9(4):e94492. (PMID: 24727794) Am J Trop Med Hyg. 2017 Dec;97(6):1650-1661. (PMID: 29141759) J Immunol. 2010 Dec 15;185(12):7426-34. (PMID: 21057084) Immunity. 2011 May 27;34(5):629-36. (PMID: 21616433) Curr Med Chem. 2012;19(10):1567-86. (PMID: 22360486) J Immunol. 2011 May 15;186(10):5766-71. (PMID: 21490162) J Immunol. 2020 Aug 1;205(3):731-740. (PMID: 32571840) PLoS One. 2014 Mar 24;9(3):e93072. (PMID: 24663956) J Exp Med. 2019 Dec 2;216(12):2714-2723. (PMID: 31582416) Nat Commun. 2019 Mar 29;10(1):1429. (PMID: 30926803) Clin Microbiol Rev. 2011 Jul;24(3):459-68. (PMID: 21734243) Parasitol Int. 2008 Jun;57(2):201-11. (PMID: 18295534) J Immunol. 2006 Jun 1;176(11):6918-27. (PMID: 16709852) Science. 2015 May 22;348(6237):aaa6566. (PMID: 25999512) Cell Rep. 2021 Jan 12;34(2):108621. (PMID: 33440150) J Immunol. 2011 Jun 1;186(11):6129-35. (PMID: 21515793) PLoS Negl Trop Dis. 2017 May 9;11(5):e0005596. (PMID: 28486498) J Immunol. 2002 May 15;168(10):5199-206. (PMID: 11994476) J Exp Med. 1995 Oct 1;182(4):931-40. (PMID: 7561696) J Immunol. 1998 Aug 1;161(3):1428-32. (PMID: 9686607) PLoS One. 2014 Sep 25;9(9):e108649. (PMID: 25255226) J Clin Invest. 2021 Mar 1;131(5):. (PMID: 33434186) Front Immunol. 2021 Nov 18;12:777860. (PMID: 34868049) Parasit Vectors. 2015 Jul 25;8:396. (PMID: 26209319) Proc Natl Acad Sci U S A. 2016 Sep 6;113(36):10139-44. (PMID: 27551096) Mem Inst Oswaldo Cruz. 1992;87 Suppl 5:83-9. (PMID: 1342722) PLoS Negl Trop Dis. 2016 Apr 25;10(4):e0004605. (PMID: 27111140) Exp Parasitol. 2015 Dec;159:118-26. (PMID: 26394284) Scand J Immunol. 2010 Aug;72(2):118-27. (PMID: 20618770) PLoS Pathog. 2014 Mar 13;10(3):e1003988. (PMID: 24626328) PLoS Negl Trop Dis. 2019 Jan 16;13(1):e0006356. (PMID: 30650071) Trans R Soc Trop Med Hyg. 1979;73(6):673-6. (PMID: 538808) |
| معلومات مُعتمدة: | MR/L018756/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: Brugia malayi; Rag2 knockout (KO) mouse; eosinophils; innate lymphoid cells (ILC); lymphatic filariasis; natural killer cells (NK cells); neutrophils |
| المشرفين على المادة: | 0 (Antigens, Ly) 0 (Interleukin-1 Receptor-Like 1 Protein) 0 (Natural Cytotoxicity Triggering Receptor 1) 0 (Ncr1 protein, mouse) 82115-62-6 (Interferon-gamma) |
| تواريخ الأحداث: | Date Created: 20221014 Date Completed: 20221017 Latest Revision: 20230315 |
| رمز التحديث: | 20230315 |
| مُعرف محوري في PubMed: | PMC9551455 |
| DOI: | 10.3389/fimmu.2022.969340 |
| PMID: | 36238293 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
|---|---|
| DOI: | 10.3389/fimmu.2022.969340 |