دورية أكاديمية
ADD10 protects renal cells from cold injuries by improving energy metabolism.
| العنوان: | ADD10 protects renal cells from cold injuries by improving energy metabolism. |
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| المؤلفون: | Cassim S; Addmedica, 16 rue Montrosier, 92200, Neuilly-Sur-Seine, France. Electronic address: shamir.cassim@addmedica.com., Martin PY; GT GAIN THERAPEUTICS SA, Via Francesco Soave 6, 6900, Lugano, Switzerland., Pascolo-Rebouillat E; Addmedica, 16 rue Montrosier, 92200, Neuilly-Sur-Seine, France. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 Dec 17; Vol. 634, pp. 62-69. Date of Electronic Publication: 2022 Oct 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Organ Preservation Solutions*/pharmacology , Reperfusion Injury*/prevention & control , Kidney Transplantation* , Cold Injury*, Swine ; Animals ; Humans ; Kidney/physiology ; LLC-PK1 Cells ; Energy Metabolism ; Insulin ; Glutathione ; Allopurinol ; Cold Temperature |
| مستخلص: | Static cold storage (SCS) is currently the most widely used method for organ preservation, but a number of limitations are associated including tissue damage and restricted opportunity for organ repair. Thus, the development of improved hypothermic storage solutions is an urgent need. Herein, using a renal epithelial cell model (LLC-PK1), we tested the benefits of ADD10, a novel clinical grade antioxidant product, in reducing damages associated with ischemia-reperfusion (IR). Cells were stored up to 24h at 4 °C in University of Wisconsin (UW) solution without or in the presence of 1% ADD10 with following reperfusion up to 24h at 37 °C. The presence of ADD10 significantly decreased cells damages, cell death, and the level of reactive oxygen species (ROS) (P < 0.05). Concomitantly, ADD10 supplementation also favored an increased oxygen consumption rate (OCR) and improved bioenergetics of LLC-PK1 cells (P < 0.05). Finally, preliminary in vivo studies suggested a benefit of ADD10 on the renal function post-transplantation. In conclusion, these results demonstrate that the addition of ADD10 to the preservation solution not only efficiently protects renal cells during SCS, but also improves the functionality of cold-stored organs during transplantation. Competing Interests: Declaration of competing interest Shamir Cassim and Emanuelle Pascolo-Rebouillat are employees of Addmedica, and importantly, the funding body had no influence on data acquisition. Pierre-Yves Martin was a former employee of Addmedica. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antioxidative compound; Ischemia-reperfusion injury; Kidneys; Organs; Preservation; Static cold storage; Transplantation |
| المشرفين على المادة: | 0 (Organ Preservation Solutions) 0 (Insulin) GAN16C9B8O (Glutathione) 63CZ7GJN5I (Allopurinol) |
| تواريخ الأحداث: | Date Created: 20221014 Date Completed: 20221108 Latest Revision: 20221206 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.bbrc.2022.10.009 |
| PMID: | 36240650 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2022.10.009 |