دورية أكاديمية
Effect of paclitaxel treatment on cellular mechanics and morphology of human oesophageal squamous cell carcinoma in 2D and 3D environments.
| العنوان: | Effect of paclitaxel treatment on cellular mechanics and morphology of human oesophageal squamous cell carcinoma in 2D and 3D environments. |
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| المؤلفون: | Kiwanuka M; Biomedical Engineering Research Centre, Division of Biomedical Engineering, Department of Human Biology, University of Cape Town, Observatory, South Africa., Higgins G; Biomedical Engineering Research Centre, Division of Biomedical Engineering, Department of Human Biology, University of Cape Town, Observatory, South Africa., Ngcobo S; Cardiovascular Research Unit, Christiaan Barnard Division of Cardiothoracic Surgery, University of Cape Town, Observatory, South Africa., Nagawa J; Biomedical Engineering Research Centre, Division of Biomedical Engineering, Department of Human Biology, University of Cape Town, Observatory, South Africa., Lang DM; Division of Cell Biology, Department of Human Biology, University of Cape Town, Observatory, South Africa., Zaman MH; Department of Biomedical Engineering, Boston University, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Davies NH; Cardiovascular Research Unit, Christiaan Barnard Division of Cardiothoracic Surgery, University of Cape Town, Observatory, South Africa., Franz T; Biomedical Engineering Research Centre, Division of Biomedical Engineering, Department of Human Biology, University of Cape Town, Observatory, South Africa.; Bioengineering Science Research Group, Engineering Sciences, University of Southampton, Southampton, UK. |
| المصدر: | Integrative biology : quantitative biosciences from nano to macro [Integr Biol (Camb)] 2022 Oct 15. Date of Electronic Publication: 2022 Oct 15. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101478378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1757-9708 (Electronic) Linking ISSN: 17579694 NLM ISO Abbreviation: Integr Biol (Camb) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Oxford : Oxford University Press Original Publication: Cambridge : RSC Publishing, c2009-c2018. |
| مستخلص: | During chemotherapy, structural and mechanical changes in malignant cells have been observed in several cancers, including leukaemia and pancreatic and prostate cancer. Such cellular changes may act as physical biomarkers for chemoresistance and cancer recurrence. This study aimed to determine how exposure to paclitaxel affects the intracellular stiffness of human oesophageal cancer of South African origin in vitro. A human oesophageal squamous cell carcinoma cell line WHCO1 was cultured on glass substrates (2D) and in collagen gels (3D) and exposed to paclitaxel for up to 48 h. Cellular morphology and stiffness were assessed with confocal microscopy, visually aided morpho-phenotyping image recognition and mitochondrial particle tracking microrheology at 24 and 48 h. In the 2D environment, the intracellular stiffness was higher for the paclitaxel-treated than for untreated cells at 24 and 48 h. In the 3D environment, the paclitaxel-treated cells were stiffer than the untreated cells at 24 h, but no statistically significant differences in stiffness were observed at 48 h. In 2D, paclitaxel-treated cells were significantly larger at 24 and 48 h and more circular at 24 but not at 48 h than the untreated controls. In 3D, there were no significant morphological differences between treated and untreated cells. The distribution of cell shapes was not significantly different across the different treatment conditions in 2D and 3D environments. Future studies with patient-derived primary cancer cells and prolonged drug exposure will help identify physical cellular biomarkers to detect chemoresistance onset and assess therapy effectiveness in oesophageal cancer patients. (© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| References: | Methods. 2017 Feb 15;115:80-90. (PMID: 27713081) Lancet. 2017 Nov 25;390(10110):2383-2396. (PMID: 28648400) Biochem Pharmacol. 1999 Jun 1;57(11):1215-21. (PMID: 10230764) Br J Cancer. 1994 Aug;70(2):335-41. (PMID: 7914426) Integr Biol (Camb). 2018 Apr 23;10(4):232-241. (PMID: 29620778) Sci Rep. 2019 Feb 7;9(1):1660. (PMID: 30733487) Cancer Res. 1996 Feb 15;56(4):816-25. (PMID: 8631019) CA Cancer J Clin. 2018 Nov;68(6):394-424. (PMID: 30207593) Nat Rev Cancer. 2005 Jul;5(7):516-25. (PMID: 15965493) Bioinformatics. 2017 Aug 01;33(15):2424-2426. (PMID: 28369169) Mol Cancer. 2013 Mar 27;12:24. (PMID: 23537295) Mol Biol Cell. 2014 Sep 15;25(18):2677-81. (PMID: 25213191) Clin Oncol (R Coll Radiol). 2011 Apr;23(3):182-8. (PMID: 21232928) Br J Cancer. 2006 May 22;94(10):1389-94. (PMID: 16670722) FEBS Open Bio. 2019 Sep;9(9):1652-1669. (PMID: 31250984) Nat Protoc. 2021 Feb;16(2):754-774. (PMID: 33424024) Cell Biochem Funct. 2018 Jan;36(1):27-33. (PMID: 29314203) Nat Methods. 2012 Jan 08;9(2):185-8. (PMID: 22231642) Br J Cancer. 2007 May 7;96(9):1348-52. (PMID: 17437008) PLoS Comput Biol. 2014 Nov 20;10(11):e1003959. (PMID: 25412385) J Cell Sci. 2009 Aug 1;122(Pt 15):2579-85. (PMID: 19625502) Br J Cancer. 2002 Mar 4;86(5):669-73. (PMID: 11875723) Am J Gastroenterol. 2017 Aug;112(8):1247-1255. (PMID: 28585555) BMC Cancer. 2016 Feb 15;16:101. (PMID: 26878873) Cells. 2018 Sep 20;7(10):. (PMID: 30241323) Biophys J. 2010 Oct 6;99(7):2048-57. (PMID: 20923638) J Thorac Dis. 2014 Jul;6(7):949-57. (PMID: 25093092) PLoS One. 2015 May 01;10(5):e0126107. (PMID: 25932632) Lancet. 2013 Feb 2;381(9864):400-12. (PMID: 23374478) Cell Death Dis. 2018 Feb 14;9(2):239. (PMID: 29445159) J Cell Biol. 2014 Jan 20;204(2):231-45. (PMID: 24446482) Cell. 2014 Aug 14;158(4):822-832. (PMID: 25126787) Sci Rep. 2015 Dec 17;5:18437. (PMID: 26675084) |
| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust; 108473/Z/15/Z United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: chemoresistance; chemotherapy; collagen; image recognition; mechanobiology; mitochondrial particle tracking microrheology; oesophageal cancer; physical biomarker |
| تواريخ الأحداث: | Date Created: 20221016 Latest Revision: 20240216 |
| رمز التحديث: | 20240217 |
| مُعرف محوري في PubMed: | PMC9585394 |
| DOI: | 10.1093/intbio/zyac013 |
| PMID: | 36244059 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1757-9708 |
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| DOI: | 10.1093/intbio/zyac013 |