دورية أكاديمية
Comparative metagenomic analysis of human intervertebral disc nucleus pulposus and cartilaginous end plates.
| العنوان: | Comparative metagenomic analysis of human intervertebral disc nucleus pulposus and cartilaginous end plates. |
|---|---|
| المؤلفون: | Shanmuganathan R; Department of Spine Surgery, Ganga Hospital, Coimbatore, India., Tangavel C; Department of Biotechnology, Ganga Research Centre, Coimbatore, India., K S SVA; Department of Spine Surgery, Ganga Hospital, Coimbatore, India., Muthurajan R; Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India., Nayagam SM; Department of Biotechnology, Ganga Research Centre, Coimbatore, India., Matchado MS; Department of Biotechnology, Ganga Research Centre, Coimbatore, India., Rajendran S; Department of Biotechnology, Ganga Research Centre, Coimbatore, India., Kanna RM; Department of Spine Surgery, Ganga Hospital, Coimbatore, India., Shetty AP; Department of Spine Surgery, Ganga Hospital, Coimbatore, India. |
| المصدر: | Frontiers in cardiovascular medicine [Front Cardiovasc Med] 2022 Sep 28; Vol. 9, pp. 927652. Date of Electronic Publication: 2022 Sep 28 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101653388 Publication Model: eCollection Cited Medium: Print ISSN: 2297-055X (Print) Linking ISSN: 2297055X NLM ISO Abbreviation: Front Cardiovasc Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Study Design: The diversity of microflora inhabiting endplate (EP) and nucleus pulposus (NP) tissues of human intervertebral disc (IVD) was profiled through NGS-supported 16S rRNA amplicon sequencing. Sixteen EP and their corresponding NP were excised from the brain-dead voluntary organ donors with no clinical history of low back pain, and 12 herniated and 8 degenerated NP tissues isolated from the patients undergoing spinal surgery were subjected to study the alteration in the microbial diversity. Objectives: To understand in normal IVD, whether the colonization of bacteria to the NP is through the EP in discs with intact annulus fibrosus. To identify significantly differing microbial population(s) between normal and diseased IVD (NP). Background of the Study: There is increasing evidence for subclinical infection by fastidious low, growing bacteria to be a cause of disc degeneration. Although the presence of bacteria in NP has been reported well in literature, the source of bacteria is not clearly proved as the disc is avascular in healthy condition. Documentation of similar bacterial populations in the EP and NP may add proof that bacterial inoculation of NP occurs via the EP. Materials and Methods: Sixteen EP and their corresponding NP excised from brain-dead voluntary organ donors with no history of back pain and 20 diseased discs collected from patients undergoing microdiscectomy/fusion surgery were used for profiling microbiome through 16S rRNA amplicon sequencing using primers specific for V1-V9 hypervariable regions. Changes in bacterial diversity and abundance were analysed to identify the key microbial populations in normal IVD NP and EP tissues and those significantly altered in diseased IVD (NP). Results: NP and EP shared a similar spectrum of microbiome but with varying abundance. The five dominant phyla identified were Proteobacteria, Firmicutes, Actinobacteria, OD1, and Bacteroidetes. Proteobacteria was found to be the most abundant phyla in both NP (62%) and EP (53%) of the normal IVD. This was followed by Firmicutes (16%), Actinobacteriota (11%), OD1 (Parcubacteria) (7.6%), and Bacteroidetes (2%) in NP and Firmicutes (23.4%), OD1 (Parcubacteria) (17.6%), Actinobacteriota (2.8%), and Bacteroidetes (2.6%) in EP, respectively . Under diseased conditions, Proteobacteria (68%) was dominant when compared with other phyla. However, there was no significant difference in the abundance of Proteobacteria between the normal and diseased discs. Interestingly, the other dominant phyla such as Firmicutes (Normal-NP: 16.2%; Diseased-NP: 4.02%) and Actinobacteria (Normal-NP: 11%; Diseased-NP: 0.99%) showed a significant reduction in degenerated discs. To understand the key microbial populations that are significantly altered during disease, correlation analysis was performed among the three phyla, which revealed a negative correlation in the ratio of Actinobacteria + Firmicutes vs. Proteobacteria ( p = 0.001) in DD. Conclusion: Results of our study clearly demonstrated a similar bacterial diversity but with varying abundance between the EP and NP, suggesting the existence of the endplate-nucleus pulposus axis in the normal IVD microbiome. Further, our results have indicated that the changes in the abundance of Actinobacteria + Firmicutes vs. Proteobacteria during DDD need further investigation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Shanmuganathan, Tangavel, K S, Muthurajan, Nayagam, Matchado, Rajendran, Kanna and Shetty.) |
| References: | Eur Spine J. 2019 Nov;28(11):2478-2486. (PMID: 31309333) Nat Methods. 2016 Jul;13(7):581-3. (PMID: 27214047) BMC Microbiol. 2020 Jul 22;20(1):221. (PMID: 32698765) Connect Tissue Res. 1981;8(2):101-19. (PMID: 6453689) Appl Microbiol Biotechnol. 2013 Feb;97(4):1475-88. (PMID: 23324802) Curr Opin Gastroenterol. 2015 Jan;31(1):69-75. (PMID: 25394236) Front Endocrinol (Lausanne). 2020 Sep 02;11:605. (PMID: 32982987) Eur Spine J. 2019 Dec;28(12):2941-2950. (PMID: 31312913) Eur Spine J. 2020 Jul;29(7):1621-1640. (PMID: 32409889) Scand J Trauma Resusc Emerg Med. 2019 Feb 14;27(1):19. (PMID: 30764843) Eur Spine J. 2008 May;17(5):626-43. (PMID: 18357472) PLoS One. 2013 Apr 22;8(4):e61217. (PMID: 23630581) Ann Transl Med. 2020 Mar;8(6):299. (PMID: 32355743) J Hosp Infect. 2007 Jul;66(3):275-7. (PMID: 17573158) Clin J Am Soc Nephrol. 2019 May 7;14(5):692-701. (PMID: 30962186) Microorganisms. 2019 Jan 10;7(1):. (PMID: 30634578) Spine (Phila Pa 1976). 2001 Dec 1;26(23):2543-9. (PMID: 11725234) Appl Environ Microbiol. 2006 Jul;72(7):5069-72. (PMID: 16820507) Spine J. 2020 Jan;20(1):48-59. (PMID: 31125691) Spine J. 2022 May 13;:. (PMID: 35569807) J Bone Joint Surg Am. 2006 Apr;88 Suppl 2:30-5. (PMID: 16595440) Front Microbiol. 2020 Feb 07;11:36. (PMID: 32117095) Oncogene. 2020 Jun;39(26):4925-4943. (PMID: 32514151) JOR Spine. 2020 Sep 15;3(4):e1123. (PMID: 33392458) Front Microbiol. 2019 Jun 21;10:1409. (PMID: 31293547) Lancet. 2018 Jun 9;391(10137):2356-2367. (PMID: 29573870) Pain. 2019 Nov;160(11):2589-2602. (PMID: 31219947) Spine (Phila Pa 1976). 2004 Dec 1;29(23):2700-9. (PMID: 15564919) PeerJ. 2017 Jan 11;5:e2836. (PMID: 28097056) Microorganisms. 2019 Nov 11;7(11):. (PMID: 31717915) Folia Morphol (Warsz). 2015;74(2):157-68. (PMID: 26050801) Genome Res. 2009 Dec;19(12):2317-23. (PMID: 19819907) Int J Environ Res Public Health. 2018 Aug 07;15(8):. (PMID: 30087270) Nutr Clin Care. 2002 Mar-Apr;5(2):56-65. (PMID: 12134711) Biomed Res Int. 2017;2017:9351507. (PMID: 29230419) |
| فهرسة مساهمة: | Keywords: bacteria; disc degeneration; dysbiosis; endplate; intervertebral disc; microbiome; next-generation sequencing |
| تواريخ الأحداث: | Date Created: 20221017 Latest Revision: 20221019 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC9554234 |
| DOI: | 10.3389/fcvm.2022.927652 |
| PMID: | 36247458 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2297-055X |
|---|---|
| DOI: | 10.3389/fcvm.2022.927652 |