دورية أكاديمية
Feeder cell-dependent primary culture of single blastula-derived embryonic cell lines from marine medaka (Oryzias dancena).
| العنوان: | Feeder cell-dependent primary culture of single blastula-derived embryonic cell lines from marine medaka (Oryzias dancena). |
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| المؤلفون: | Son MJ; Department of Fisheries Biology, Pukyong National University, Busan, 48513, Korea., Gong SP; Department of Fisheries Biology, Pukyong National University, Busan, 48513, Korea. gongsp@pknu.ac.kr.; Division of Fisheries Life Science, Major in Aquaculture and Applied Life Science, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan, 48513, Korea. gongsp@pknu.ac.kr. |
| المصدر: | In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2022 Oct; Vol. 58 (9), pp. 840-850. Date of Electronic Publication: 2022 Oct 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9418515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-706X (Electronic) Linking ISSN: 10712690 NLM ISO Abbreviation: In Vitro Cell Dev Biol Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Original Publication: Columbia, MD : Tissue Culture Association, c1993- |
| مواضيع طبية MeSH: | Oryzias*, Animals ; Feeder Cells ; Blastula ; Cell Differentiation ; Cell Line |
| مستخلص: | Fish embryonic stem cells (ESCs) are derived from blastomeres that have been cultured from blastula embryos. The most widely used method for derivation of fish ESCs is the culture of blastomeres that have been isolated from approximately 10 blastula embryos under feeder-free conditions. However, this method leads to intercellular genetic heterogeneity among the cultured cells, which is a major obstacle to the development of stable ESC culture conditions. In this study, to establish ESC lines with intercellular genetic homogeneity at the early stage of culture, we attempted to derive embryonic cell lines from single blastula-derived blastomeres of marine medaka (Oryzias dancena) in a feeder cell culture system. Using basic fibroblast growth factor-expressing feeder cells during primary culture, we successfully established 22 single blastula-derived embryonic cell lines that could be subcultured more than 20 times. In contrast, we were unable to efficiently derive cell lines using wild-type feeder cells and under feeder-free conditions. The established cell lines exhibited ESC-like cell characteristics in terms of alkaline phosphatase activity, pluripotency-related gene expression, and embryoid body formation. The results of this study will contribute to the development of methods for derivation of fish ESCs. (© 2022. The Society for In Vitro Biology.) |
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| معلومات مُعتمدة: | 2021 Pukyong National University |
| فهرسة مساهمة: | Keywords: Basic fibroblast growth factor; Cell lines; Cellular homogeneity; Embryonic stem cells; Feeder cells |
| تواريخ الأحداث: | Date Created: 20221017 Date Completed: 20221201 Latest Revision: 20221201 |
| رمز التحديث: | 20221201 |
| DOI: | 10.1007/s11626-022-00727-1 |
| PMID: | 36251152 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1543-706X |
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| DOI: | 10.1007/s11626-022-00727-1 |