دورية أكاديمية
أعرض في EDS

Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts.

التفاصيل البيبلوغرافية
العنوان: Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts.
المؤلفون: Jensen ED; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark. emdaje@biosustain.dtu.dk., Deichmann M; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Ma X; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Vilandt RU; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Schiesaro G; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Rojek MB; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Lengger B; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Eliasson L; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Vento JM; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA., Durmusoglu D; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA., Hovmand SP; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Al'Abri I; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA., Zhang J; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Crook N; Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA., Jensen MK; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark. mije@biosustain.dtu.dk.
المصدر: Nature communications [Nat Commun] 2022 Oct 19; Vol. 13 (1), pp. 6201. Date of Electronic Publication: 2022 Oct 19.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Saccharomyces cerevisiae*/metabolism , Probiotics*, Reproduction/genetics ; Receptors, G-Protein-Coupled/metabolism ; Pheromones/metabolism ; Receptors, Cell Surface/metabolism ; Cell Differentiation ; Cell Communication ; Ligands
مستخلص: G protein-coupled receptors (GPCRs) enable cells to sense environmental cues and are indispensable for coordinating vital processes including quorum sensing, proliferation, and sexual reproduction. GPCRs comprise the largest class of cell surface receptors in eukaryotes, and for more than three decades the pheromone-induced mating pathway in baker's yeast Saccharomyces cerevisiae has served as a model for studying heterologous GPCRs (hGPCRs). Here we report transcriptome profiles following mating pathway activation in native and hGPCR-signaling yeast and use a model-guided approach to correlate gene expression to morphological changes. From this we demonstrate mating between haploid cells armed with hGPCRs and endogenous biosynthesis of their cognate ligands. Furthermore, we devise a ligand-free screening strategy for hGPCR compatibility with the yeast mating pathway and enable hGPCR-signaling in the probiotic yeast Saccharomyces boulardii. Combined, our findings enable new means to study mating, hGPCR-signaling, and cell-cell communication in a model eukaryote and yeast probiotics.
(© 2022. The Author(s).)
References: Bioinformatics. 2015 Jan 15;31(2):166-9. (PMID: 25260700)
Sci Adv. 2017 Jun 28;3(6):e1603221. (PMID: 28782007)
Cell. 2019 Apr 18;177(3):782-796.e27. (PMID: 30955892)
Biotechnol J. 2016 Aug;11(8):1110-7. (PMID: 27166612)
Science. 1990 Oct 5;250(4977):121-3. (PMID: 2171146)
Mol Biol Cell. 1999 Aug;10(8):2559-72. (PMID: 10436012)
Genome Res. 2019 Sep;29(9):1478-1494. (PMID: 31467028)
FEBS Lett. 2012 Nov 30;586(23):4208-4214. (PMID: 23108052)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
FEMS Yeast Res. 2020 Feb 1;20(1):. (PMID: 31825496)
ACS Synth Biol. 2021 Mar 19;10(3):466-477. (PMID: 33577304)
Annu Rev Genet. 1998;32:561-99. (PMID: 9928492)
Mol Cell Proteomics. 2004 May;3(5):478-89. (PMID: 14766929)
Appl Microbiol Biotechnol. 2015 Feb;99(3):1299-308. (PMID: 25331280)
Biotechnol J. 2016 May;11(5):717-24. (PMID: 26710256)
Mol Cell Biol. 1982 Jan;2(1):11-20. (PMID: 7050665)
Mol Syst Biol. 2019 Dec;15(12):e9021. (PMID: 31885202)
J Cell Biol. 1980 Jun;85(3):811-22. (PMID: 6993497)
Proc Natl Acad Sci U S A. 2020 Jun 9;117(23):13117-13126. (PMID: 32434907)
Therap Adv Gastroenterol. 2012 Mar;5(2):111-25. (PMID: 22423260)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
Nature. 1998 Dec 3;396(6710):422-3. (PMID: 9853747)
Yeast. 2000 Jan 15;16(1):11-22. (PMID: 10620771)
PLoS One. 2008;3(12):e3865. (PMID: 19052645)
Science. 2000 Feb 4;287(5454):873-80. (PMID: 10657304)
ACS Synth Biol. 2021 May 21;10(5):1039-1052. (PMID: 33843197)
J Biol Chem. 2002 Jul 5;277(27):24515-21. (PMID: 11923301)
Nat Protoc. 2013 Sep;8(9):1765-86. (PMID: 23975260)
ACS Synth Biol. 2015 Sep 18;4(9):975-86. (PMID: 25871405)
Proc Natl Acad Sci U S A. 1943 Oct 15;29(10):306-8. (PMID: 16588616)
ACS Sens. 2022 May 27;7(5):1323-1335. (PMID: 35452231)
Br J Pharmacol. 2014 Aug;171(15):3651-65. (PMID: 24712679)
Genome Biol. 2010;11(3):R25. (PMID: 20196867)
Nat Med. 2021 Jul;27(7):1212-1222. (PMID: 34183837)
Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308)
J Biol Chem. 1997 Feb 14;272(7):3871-4. (PMID: 9064301)
Metab Eng. 2015 Mar;28:213-222. (PMID: 25638686)
Nat Commun. 2018 Nov 29;9(1):5057. (PMID: 30498215)
Genome Res. 2004 Jun;14(6):1043-51. (PMID: 15173111)
Mol Biol Cell. 1998 Dec;9(12):3273-97. (PMID: 9843569)
Curr Biol. 2011 Aug 23;21(16):1337-46. (PMID: 21835624)
Nucleic Acids Res. 2021 Sep 7;49(15):e88. (PMID: 34107026)
Genetics. 2013 Dec;195(4):1277-90. (PMID: 24121774)
Microb Cell Fact. 2017 Mar 15;16(1):46. (PMID: 28298224)
Nucleic Acids Res. 2013 Apr;41(7):4336-43. (PMID: 23460208)
Nat Rev Genet. 2001 Sep;2(9):659-68. (PMID: 11533715)
Proc Natl Acad Sci U S A. 1953 Mar;39(3):171-9. (PMID: 16589243)
Fungal Genet Biol. 2005 Apr;42(4):328-38. (PMID: 15749052)
Nucleic Acids Res. 2007;35(6):1992-2002. (PMID: 17341463)
معلومات مُعتمدة: P30 DK034987 United States DK NIDDK NIH HHS; T32 GM008776 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Receptors, G-Protein-Coupled)
0 (Pheromones)
0 (Receptors, Cell Surface)
0 (Ligands)
تواريخ الأحداث: Date Created: 20221019 Date Completed: 20221021 Latest Revision: 20240520
رمز التحديث: 20240520
مُعرف محوري في PubMed: PMC9582028
DOI: 10.1038/s41467-022-33961-y
PMID: 36261657
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-022-33961-y