دورية أكاديمية
Diversity of Haemogregarine Parasites Infecting Brazilian Anurans, with a Description of New Species of Dactylosoma (Apicomplexa: Adeleorina: Dactylosomatidae).
العنوان: | Diversity of Haemogregarine Parasites Infecting Brazilian Anurans, with a Description of New Species of Dactylosoma (Apicomplexa: Adeleorina: Dactylosomatidae). |
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المؤلفون: | Úngari LP; Setor de Parasitologia, DBBVPZ, Instituto de Biociências, Universidade Estadual Paulista- UNESP, Distrito de Rubião Junior, Botucatu, São Paulo, Brasil. letspungari@hotmail.com., Netherlands EC; Department of Zoology and Entomology, University of the Free State, PO Box 339, Bloemfontein, 9300, South Africa., Santos ALQ; Laboratório de Ensino e Pesquisa em Animais Silvestres, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brasil., de Alcantara EP; Setor de Parasitologia, DBBVPZ, Instituto de Biociências, Universidade Estadual Paulista- UNESP, Distrito de Rubião Junior, Botucatu, São Paulo, Brasil., Emmerich E; Setor de Parasitologia, DBBVPZ, Instituto de Biociências, Universidade Estadual Paulista- UNESP, Distrito de Rubião Junior, Botucatu, São Paulo, Brasil., da Silva RJ; Setor de Parasitologia, DBBVPZ, Instituto de Biociências, Universidade Estadual Paulista- UNESP, Distrito de Rubião Junior, Botucatu, São Paulo, Brasil., O Dwyer LH; Setor de Parasitologia, DBBVPZ, Instituto de Biociências, Universidade Estadual Paulista- UNESP, Distrito de Rubião Junior, Botucatu, São Paulo, Brasil. |
المصدر: | Acta parasitologica [Acta Parasitol] 2022 Dec; Vol. 67 (4), pp. 1740-1755. Date of Electronic Publication: 2022 Oct 20. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2019- : Cham : Springer International Publishing Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology |
مواضيع طبية MeSH: | Parasites* , Eucoccidiida* , Apicomplexa*/genetics, Animals ; Brazil ; Phylogeny ; Anura/parasitology |
مستخلص: | Purpose: Brazilian anurans are considered the most diverse and species rich around the world. Although in recent years there has been a strong focus on research related to this group of animals, their parasites have not received the same attention. Thus, this study aimed to provide morphological and molecular data on haemogregarines biodiversity infecting Brazilian anurans. Methods: During 2020, 116 anurans were collected from four Brazilian States and their blood and fragment of organs were screened for haemogregarine parasites. Results: From the total, seven (6.03%) animals were found infected with species of Hepatozoon and Dactylosoma. Based on the morphological and molecular analysis, four anurans were found infected with Hepatozoon latrensis. The phylogenetic analysis has shown the isolates from this study grouping with the Brazilian anuran Hepatozoon clade, also with gene similarity ranging from 99.70 to 100% to H. latrensis isolates available on GenBank. Furthermore, three specimens (Trachycephalus typhonius, Leptodactylus latrans, and Rhinella diptycha) were infected with the same species of Dactylosoma (100% genetic similarity), with a genetic similarity of 98.56% to Dactylosoma piperis the only other species described in Brazil. In support of the molecular data, different morphological characters were observed in the blood smears as compared to D. piperis, suggesting that the species of Dactylosoma from the present study infecting three different species of Brazilian anurans is an undescribed species. Conclusion: Thus, this study increases the knowledge of Brazilian anuran blood parasites and demonstrates the importance of using integrative approaches for the diagnosis of haemoparasites. (© 2022. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.) |
References: | Wake D, Vredenburg VT (2008) Are we in the midst of the sixth mass extinction? A view from the world of amphibians. Proc Natl Acad Sci USA 105:11466–11473. https://doi.org/10.1073/pnas.0801921105. (PMID: 10.1073/pnas.0801921105) Isaak-Delgado AB, López-Díaz O, Romero-Callejas E, Martínez-Hernández F, Muñoz-Garcia CI, Villalobos G, Randón-Franco E (2020) Morphological and molecular characteristics of hemoparasites in vaillant’s frogs (Lithobates vaillanti). Parasitol Res 119:1891–1901. https://doi.org/10.1007/s00436-020-06689-1. (PMID: 10.1007/s00436-020-06689-1) Stuart S, Chanson J, Cox N, Young B (2006) El estado global de los anfíbios. In: Ângulo A, Rueda J, Rodríguez J, La Marca E (eds) Técnicas de inventario y monitoreo para los anfibios de la región tropical andina. Conservación internacional serie manuales para la conservación, vol 2. Panamericana Formas e Impresos S A, Bogotá, pp 19–34. Netherlands EC, Cook CA, Smit NJ (2014) Hepatozoon species [Adeleorina: Hepatozoidae] of African bufonids, with morphological description and molecular diagnosis of Hepatozoon ixoxo sp. nov. parasiting three Amietophrynus species (Anura: Bufonid). Parasit Vectors 7:552–563. https://doi.org/10.1186/s13071-014-0552-0. (PMID: 10.1186/s13071-014-0552-0) Barta JR, Ogedengbe JD, Martin DS, Smith TG (2012) Phylogenetic position of the Adeleorinid Coccidia (Myzozoa, Apicomplexa, Coccidia, Eucoccidiorida, Adeleorina) inferred using 18S rDNA sequences. J Eukaryot Microbiol 59:171–180. https://doi.org/10.1111/j.1550-7408.2011.00607.x. (PMID: 10.1111/j.1550-7408.2011.00607.x) Davies A, Johnston M (2000) The biology of some intraerythrocytic parasites of fishes, Amphibia and reptiles. Adv Parasitol 45:1–107. https://doi.org/10.1016/S0065-308X(00)45003-7. (PMID: 10.1016/S0065-308X(00)45003-7) Netherlands EC, Cook CA, Smit NJ, Du Preez LH (2014) Redescription and molecular diagnosis of Hepatozoon theileri (Laveran, 1905) (Apicomplexa: Adeleorina: Hepatozoidae) infecting Amietia quecketti (Anura: Pyxicephalidae). Folia Parasitol 61:239–300. https://doi.org/10.14411/fp.2014.046. (PMID: 10.14411/fp.2014.046) Levine ND (1988) The protozoan phylum apicomplexan, vol 2. CRC Press, Boca Raton. Netherlands EC, Cook CA, Du Preez LH (2018) Monophyly of the species of Hepatozoon (Adeleorina: Hepatozoidae) parasiting (African) anurans, with the description of three new species from hyperoliid frogs in South Africa. Parasitol 145:1039–1050. https://doi.org/10.1017/S003118201700213X. (PMID: 10.1017/S003118201700213X) Úngari LP, Netherlands EC, Santos ALQ, Alcantara EP, Emmerich E, Silva RJ, O´Dwyer LH (2021) New insights on the diversity of Brazilian anuran blood parasites: with the description of three new species of Hepatozoon (Apicomplexa:Hepatozoidae) from Leptodactylidae anurans. Int J Parasitol 14:190–201. https://doi.org/10.1016/j.ijppaw.2021.02.009. (PMID: 10.1016/j.ijppaw.2021.02.009) Costa SCG, Pessoa SB, Pereira NM, Colombo T (1973) The life history of Hepatozoon leptodactyli (Lesage, 19080) Pessoa, 1970: a parasite of the common laboratory animal – the frog of the genus Leptodactylus. Mem Inst Oswaldo Cruz 71:1–18. https://doi.org/10.1590/S0074-02761973000100001. (PMID: 10.1590/S0074-02761973000100001) Netherlands EC, Cook CA, Du Preez LH, Vanhove MPM, Bren- donck L, Smit NJ, (2020) An overview of the Dactylosomatidae (Apicomplexa: Adeleorina: Dactylosomatidae), with the description of Dactylosoma kermiti n. sp. parasitising Ptychadena anchietae and Sclerophrys gutturalis from South Africa. Int J Parasitol 11:246–260. https://doi.org/10.1016/j.ijppaw.2019.12.006. (PMID: 10.1016/j.ijppaw.2019.12.006) Zippel KC, Lillywhite HB, Mladinich CR (2001) New vascular system in reptiles: anatomy and postural hemodynamics of the vertebral venous plexus in snakes. J Morphol 250:173–184. https://doi.org/10.1002/jmor.1063. (PMID: 10.1002/jmor.1063) Sebben A (2007) Microdissecção fisiológica a fresco: uma nova visão sobre a anatomia de anfíbios e répteis. In: Nascimento LB, Oliveira ME (eds) Herpetologia no Brasil, vol 2. Sociedade Brasileira de Herpetologia, Belo Horizonte, pp 311–325. Eisen RJ, Schall JJ (2000) Life history of malaria parasite (Plasmodium mexicanum): independent traits and basis for variation. Proc R Soc B: Biol Sci 267:793–799. https://doi.org/10.1098/rspb.2000.1073. (PMID: 10.1098/rspb.2000.1073) Cook CA, Smit NJ, Davies AJ (2009) A redescription of Haemogregarina fitzsimonsi Dias, 1953 and some comments on Haemogregarina parvula Dias, 1953 (Adeleorina: Haemogregarinidae) from Southern African tortoises (Cyptodira: Testudinidae) with new host data and distribution records. Folia Parasitol 56:173–179. https://doi.org/10.14411/fp.2009.021. (PMID: 10.14411/fp.2009.021) Ujvari B, Madsen T, Olsson M (2004) High prevalence of Hepatozoon spp. (Apicomplexa, Hepatozoidae) infection in water pythons (Liasis fuscus) from Tropical Australia. J Parasitol 90:670–672. https://doi.org/10.1645/GE-204R. (PMID: 10.1645/GE-204R) Perkins SL, Keller AK (2001) Phylogeny of nuclear small subunit rRNA genes of hemogregarines amplified with specific oligonucleotídeos. J Parasitol 87:870–876. https://doi.org/10.1645/0022-3395(2001)087[0870:PONSSR]2.0.CO;2. (PMID: 10.1645/0022-3395(2001)087[0870:PONSSR]2.0.CO;2) Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649. https://doi.org/10.1093/bioinformatics/bts199. (PMID: 10.1093/bioinformatics/bts199) Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755. https://doi.org/10.1093/bioinformatics/btg180. (PMID: 10.1093/bioinformatics/btg180) Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:312–1313. https://doi.org/10.1093/bioinformatics/btu033. (PMID: 10.1093/bioinformatics/btu033) Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Syst Biol 67:901–904. https://doi.org/10.1093/sysbio/syy032. (PMID: 10.1093/sysbio/syy032) Rambaut A (2012) FigTree v1.4.2. Available at: http://tree.bio.ed.ac.uk/software/figtree/ (Accessed Mar 2022). Úngari LP, Netherlands EC, Santos ALQ, Alcantara EP, Emmerich E, Silva RJ, O´Dwyer LH (2020) A new species, Dactylosoma piperis n. sp. (Apicomplexa, Dactylosomatidae), from the pepper frog Leptodactylus labyrinthicus (Anura, Leptodactylidae) from Mato Grosso State, Brazil. Parasite 27:73. https://doi.org/10.1051/parasite/2020070. (PMID: 10.1051/parasite/2020070) O´Dwyer LH, Moço TC, Paduan KS, Spenassatto C, Silva RJ, Ribolla PE (2013) Description of three new species of Hepatozoon (Apicomplexa, Hepatozoidae) from Rattlesnakes (Crotalus durissus terrificus) based on molecular, morphometric and morphologic characters. Exp Parasitol 135:200–207. https://doi.org/10.1016/j.exppara.2013.06.019. (PMID: 10.1016/j.exppara.2013.06.019) ICZN, International Code of Zoological Nomenclature (1999) The International Trust for Zoological Nomenclature: London. Available at: http://www.nhm.ac.uk/hosted-sites/iczn/code/ (Accessed July 2021). Barta JR, Boulard Y, Desser SS (1987) Ultrastructural observations on secundary merogony and gametogony of Dactylosoma ranarum Labbé, 1894. J Parasitol 73:1019–1029. https://doi.org/10.2307/3282527. (PMID: 10.2307/3282527) Frost DR (2019) Amphibian species of the world. American Museum of Natural History, New York. Frost DR (2022) Amphibian species of the world: an online reference. American Museum of Natural History, New York. Telford SR Jr (2009) Hemoparasite of the reptilian: color atlas and text. CRC Press, Boca Raton. Smith TG (1996) The genus Hepatozoon (Apicomplexa: Adeleorina). J Parasitol 82:565–585. https://doi.org/10.2307/3283781. (PMID: 10.2307/3283781) Barta JR, Desser SS (1984) Blood parasites of amphibians from Algonquin Park, Ontario. J Wildl Dis 20:180–189. https://doi.org/10.7589/0090-3558-20.3.180. (PMID: 10.7589/0090-3558-20.3.180) Côelho TA, De Souza DC, Oliveira EC, Correa LL, Viana LA, Kawashita-Robeiro RC (2021) Haemogregarine of Genus Dactylosoma (Adeleorina: Dactylosomatidae) in species of Rhinella (Anura: Bufonidae) from the Brazilian Amazon. Acta Parasitol 66:1574–1580. https://doi.org/10.1007/s11686-021-00399-z. (PMID: 10.1007/s11686-021-00399-z) Ferreira DAR, Perles L, Machado RZ, Prado CPA, André MR (2020) Molecular detection of Apicomplexan hemoparasites in anurans from Brazil. Parasitol Res 119:3469–3479. https://doi.org/10.1007/s00436-020-06835-9. (PMID: 10.1007/s00436-020-06835-9) Leal DDM, Dreyer CS, Da Silva RJ, Ribolla PEM, Paduan KS, Blanchi I, O´Dwyer LH, (2015) Characterization of Hepatozoon spp. in Leptodactylus chaquensis and Leptodactylus podicipinus from two regions of the Pantanal, state of Mato Grosso do Sul, Brazil. Parasitol Res 114:1541–1549. https://doi.org/10.1007/s00436-015-4338-x. (PMID: 10.1007/s00436-015-4338-x) Netherlands EC, Cook CA, Du Kruger DJD, Du Preez LH, Smit NJ (2015) Biodiversity of frog haemoparasites from sub-tropical northern KwaZulu-Natal. South Africa, IJP:PAW 4:135–141. https://doi.org/10.1016/j.ijppaw.2015.01.003. (PMID: 10.1016/j.ijppaw.2015.01.003) Segalla M, Berneck B, Canedo C, Caramaschi U, Cruz CAG, Garcia PCA, Grant T, Haddad CFB, Lourenço AC, Mangia S, Mott T, Nascimento L, Toledo LF, Werneck F, Langone LA (2021) List of Brazilian amphibians. Herpetol Bras 10:121–216. https://doi.org/10.5281/zenodo.4716176. (PMID: 10.5281/zenodo.4716176) Lainson R, Paperna I, Naiff RD (2003) Developmental of Hepatozoon caimani (Carini, 1909) Pessoa, de Biasi and Souza, 1972 in the caiman Caiman c. crocodilus, the frog Rana catesbianae and the mosquito Culex fatigans. Mem Inst Oswaldo Cruz 98:103–113. (PMID: 10.1590/S0074-02762003000100014) Fantham HB, Porter A, Richardson LR (1942) Some haematozoa observed in vertebrates in Eastern Canada. Parasitology 34:199–226. https://doi.org/10.1017/S0031182000016176. (PMID: 10.1017/S0031182000016176) Saunders DC (1960) A survey of the blood parasites in the fishes of the Red Sea. Trans Am Micros Soc 79:239–252. https://doi.org/10.2307/3223730. (PMID: 10.2307/3223730) Manwell RD (1964) The genus Dactylosoma. J Protozool 11:526–530. https://doi.org/10.1111/j.1550-7408.1964.tb01792.x. (PMID: 10.1111/j.1550-7408.1964.tb01792.x) Kruse W (1890) Archiv fur pathologische. Anatomie Physiol fur klin Med 120:173–183. Barta JR (1991) The dactylosomatidae. Adv Parasitol 30:1–37. (PMID: 10.1016/S0065-308X(08)60305-X) Nöller W (1913) Die Blutprotozoen des wasserfrosches und ihre übertragung. Archiv fur Protistenkde 31:169–240. Boulard Y, Vivier E, Landau I (1982) Ultrastucture de Dactylosoma ranarum (Kruse, 1890); affinites avec les coccidies; revision du statut taxonomique des dactylosomides. Protistologica 18:103–112. |
معلومات مُعتمدة: | 2018/00754-9 Fapesp; 2018/09623-4 Fapesp |
فهرسة مساهمة: | Keywords: 18 S rRNA; Amphibian; Haemogregarine; Haemoparasite; Hepatozoon; Phylogeny |
تواريخ الأحداث: | Date Created: 20221020 Date Completed: 20221130 Latest Revision: 20221130 |
رمز التحديث: | 20231215 |
DOI: | 10.1007/s11686-022-00624-3 |
PMID: | 36264526 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1896-1851 |
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DOI: | 10.1007/s11686-022-00624-3 |