دورية أكاديمية
Mpp51Aa1 toxicity to Diaphorina citri nymphs demonstrated using a new, long-term bioassay method.
العنوان: | Mpp51Aa1 toxicity to Diaphorina citri nymphs demonstrated using a new, long-term bioassay method. |
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المؤلفون: | Tavares CS; Department of Entomology and Nematology, University of Florida, PO Box 110620, Gainesville, FL 32611, USA., Bonning BC; Department of Entomology and Nematology, University of Florida, PO Box 110620, Gainesville, FL 32611, USA. Electronic address: bbonning@ufl.edu. |
المصدر: | Journal of invertebrate pathology [J Invertebr Pathol] 2022 Nov; Vol. 195, pp. 107845. Date of Electronic Publication: 2022 Oct 18. |
نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0014067 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0805 (Electronic) Linking ISSN: 00222011 NLM ISO Abbreviation: J Invertebr Pathol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
مواضيع طبية MeSH: | Hemiptera*/microbiology , Rhizobiaceae* , Citrus*/microbiology, Animals ; Nymph/microbiology ; Plant Diseases/microbiology ; Biological Assay |
مستخلص: | While pesticidal proteins from Bacillus thuringiensis have provided for effective management of several insect pests of agricultural importance, few with toxicity to hemipteran species have been identified. The Asian citrus psyllid, Diaphorina citri transmits Candidatus Liberibacter asiaticus (CLas), the presumed bacterial causative agent of the devastating disease citrus greening. Despite the critical role of D. citri nymphs in the acquisition and inoculation of CLas, the lack of a long-term feeding method impedes the screening of Bt proteins for toxicity against nymphs, which play a key role in CLas transmission. Here, we developed a long-term nymph bioassay and determined the toxicity of the Bt pesticidal protein Mpp51Aa1. The new bioassay method allows nymphs to survive for up to six days when maintained on treated folded wipes. The standard hemipteran membrane feeding assay was used to assess Mpp51Aa1 toxicity against D. citri adults. Mpp51Aa1 was toxic to D. citri nymphs with a median lethal concentration (LC Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.) |
فهرسة مساهمة: | Keywords: Asian citrus psyllid; Bioassay; Diaphorina citri; Mpp51Aa1; Nymphs; Pesticidal protein |
تواريخ الأحداث: | Date Created: 20221021 Date Completed: 20221202 Latest Revision: 20221221 |
رمز التحديث: | 20240628 |
DOI: | 10.1016/j.jip.2022.107845 |
PMID: | 36270336 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1096-0805 |
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DOI: | 10.1016/j.jip.2022.107845 |