دورية أكاديمية
أعرض في EDS

Anti-inflammatory and anti-oxidant properties of Ipomoea nil (Linn.) Roth significantly alleviates cigarette smoke (CS)-induced acute lung injury via possibly inhibiting the NF-κB pathway.

التفاصيل البيبلوغرافية
العنوان: Anti-inflammatory and anti-oxidant properties of Ipomoea nil (Linn.) Roth significantly alleviates cigarette smoke (CS)-induced acute lung injury via possibly inhibiting the NF-κB pathway.
المؤلفون: Zeng LH; Department of Pharmacology, Zhejiang University City College, 51 Huzhou Street, Hangzhou 310015, China., Fatima M; Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan., Syed SK; School of Health Sciences, University of Management and Technology Lahore, Pakistan., Shaukat S; Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan., Mahdy A; Medical Pharmacology Department, International School of Medicine, Istanbul Medipol University, Istanbul, Turkey., Hussain N; Department of Pharmaceutical Sciences, College of Pharmacy, Al Ain University, Al Ain 64141, United Arab Emirates., Al Haddad AHI; Chief Operations Office, Sheikh Shakbout Medical City (SSMC), Abu Dhabi, UAE., Said ASA; Department of Clinical Pharmacy, College of Pharmacy, Al Ain University, Abu Dhabi, United Arab Emirates; AAU Health and Biomedical Research Center, Al Ain University, Abu Dhabi, United Arab Emirates; Clinical Pharmacy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., Alqahtani A; Department of Pharmacology, College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia., Alqahtani T; Department of Pharmacology, College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia., Majeed A; Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60000, Pakistan., Tariq M; Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan., Hussain M; Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan. Electronic address: musaddique.hussain@iub.edu.pk.
المصدر: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2022 Nov; Vol. 155, pp. 113267. Date of Electronic Publication: 2022 Sep 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 8213295 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1950-6007 (Electronic) Linking ISSN: 07533322 NLM ISO Abbreviation: Biomed Pharmacother Subsets: MEDLINE
أسماء مطبوعة: Publication: Paris : Editions Scientifiques Elsevier
Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
مواضيع طبية MeSH: Ipomoea nil* , Cigarette Smoking* , Acute Lung Injury*/metabolism , Respiratory Distress Syndrome*, Male ; Mice ; Animals ; NF-kappa B/metabolism ; Antioxidants/therapeutic use ; Acetylcholinesterase ; Butyrylcholinesterase ; Saline Solution/adverse effects ; Interleukin-6 ; Anti-Inflammatory Agents/therapeutic use ; Nicotiana/adverse effects ; Cytokines/metabolism ; Chemokines ; Dexamethasone/adverse effects ; Lipoxygenases/therapeutic use
مستخلص: Acute respiratory distress syndrome (ARDS), a serious manifestation of acute lung injury (ALI), is a debilitating inflammatory lung disease that is caused by multiple risk factors. One of the primary causes that can lead to ALI/ARDS is cigarette smoke (CS) and its primary mode of action is via oxidative stress. Despite extensive research, no appropriate therapy is currently available to treat ALI/ARDS, which means there is a dire need for new potential approaches. In our study we explored the protective effects of 70 % methanolic-aqueous extract of Ipomoea nil (Linn.) Roth, named as In.Mcx against CS-induced ALI mice models and RAW 264.7 macrophages because Ipomoea nil has traditionally been used to treat breathing irregularities. Male Swiss albino mice (20-25 ± 2 g) were subjected to CS for 10 uninterrupted days in order to establish CS-induced ALI murine models. Dexamethasone (1 mg/kg), In.Mcx (100 200, and 300 mg/kg) and normal saline (10 mL/kg) were given to respective animal groups, 1 h before CS-exposure. 24 h after the last CS exposure, the lungs and bronchoalveolar lavage fluid (BALF) of all euthanized mice were harvested. Altered alveolar integrity and elevated lung weight-coefficient, total inflammatory cells, oxidative stress, expression of pro-inflammatory cytokines (IL-1β and IL-6) and chemokines (KC) were significantly decreased by In.Mcx in CS-exposed mice. In.Mcx also revealed significant lowering IL-1β, IL-6 and KC expression in CSE (4 %)-activated RAW 264.7 macrophage. Additionally, In.Mcx showed marked enzyme inhibition activity against Acetylcholinesterase, Butyrylcholinesterase and Lipoxygenase. Importantly, In.Mcx dose-dependently and remarkably suppressed the CS-induced oxidative stress via not only reducing the MPO, TOS and MDA content but also improving TAC production in the lungs. Accordingly, HPLC analysis revealed the presence of many important antioxidant components. Finally, In.Mcx showed a marked decrease in the NF-κB expression both in in vivo and in vitro models. Our findings suggest that In.Mcx has positive therapeutic effects against CS-induced ALI via suppressing uncontrolled inflammatory response, oxidative stress, lipoxygenase and NF-κB p65 pathway.
(Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
فهرسة مساهمة: Keywords: Acute lung injury; Lipoxygenase pathway; NF-κB p65; Oxidative stress; Pro-inflammatory cytokines; RAW 264.7 macrophage
المشرفين على المادة: 0 (NF-kappa B)
0 (Antioxidants)
EC 3.1.1.7 (Acetylcholinesterase)
EC 3.1.1.8 (Butyrylcholinesterase)
0 (Saline Solution)
0 (Interleukin-6)
0 (Anti-Inflammatory Agents)
0 (Cytokines)
0 (Chemokines)
7S5I7G3JQL (Dexamethasone)
EC 1.13.11.- (Lipoxygenases)
تواريخ الأحداث: Date Created: 20221022 Date Completed: 20221025 Latest Revision: 20231213
رمز التحديث: 20231215
DOI: 10.1016/j.biopha.2022.113267
PMID: 36271539
قاعدة البيانات: MEDLINE
الوصف
تدمد:1950-6007
DOI:10.1016/j.biopha.2022.113267