دورية أكاديمية
Challenges for the development of a universal vaccine against leptospirosis revealed by the evaluation of 22 vaccine candidates.
العنوان: | Challenges for the development of a universal vaccine against leptospirosis revealed by the evaluation of 22 vaccine candidates. |
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المؤلفون: | Maia MAC; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Bettin EB; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Barbosa LN; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., de Oliveira NR; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Bunde TT; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Pedra ACK; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Rosa GA; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., da Rosa EEB; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Seixas Neto ACP; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., Grassmann AA; Department of Medicine, University of Connecticut Health, Farmington, CT, United States., McFadden J; School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom., Dellagostin OA; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil., McBride AJA; Biotechnology Unit, Technological Development Centre, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.; School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom. |
المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Oct 07; Vol. 12, pp. 940966. Date of Electronic Publication: 2022 Oct 07 (Print Publication: 2022). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
مواضيع طبية MeSH: | Leptospirosis*/prevention & control , Leptospira*, Cricetinae ; Humans ; Mice ; Animals ; Aluminum Hydroxide ; Reproducibility of Results ; Bacterial Vaccines ; Antigens, Bacterial/genetics ; Recombinant Proteins ; Escherichia coli ; Immunoglobulin G ; Epitopes |
مستخلص: | Leptospirosis is a neglected disease of man and animals that affects nearly half a million people annually and causes considerable economic losses. Current human vaccines are inactivated whole-cell preparations (bacterins) of Leptospira spp. that provide strong homologous protection yet fail to induce a cross-protective immune response. Yearly boosters are required, and serious side-effects are frequently reported so the vaccine is licensed for use in humans in only a handful of countries. Novel universal vaccines require identification of conserved surface-exposed epitopes of leptospiral antigens. Outer membrane β-barrel proteins (βb-OMPs) meet these requirements and have been successfully used as vaccines for other diseases. We report the evaluation of 22 constructs containing protein fragments from 33 leptospiral βb-OMPs, previously identified by reverse and structural vaccinology and cell-surface immunoprecipitation. Three-dimensional structures for each leptospiral βb-OMP were predicted by I-TASSER. The surface-exposed epitopes were predicted using NetMHCII 2.2 and BepiPred 2.0. Recombinant constructs containing regions from one or more βb-OMPs were cloned and expressed in Escherichia coli . IMAC-purified recombinant proteins were adsorbed to an aluminium hydroxide adjuvant to produce the vaccine formulations. Hamsters (4-6 weeks old) were vaccinated with 2 doses containing 50 - 125 μg of recombinant protein, with a 14-day interval between doses. Immunoprotection was evaluated in the hamster model of leptospirosis against a homologous challenge (10 - 20× ED Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Maia, Bettin, Barbosa, de Oliveira, Bunde, Pedra, Rosa, da Rosa, Seixas Neto, Grassmann, McFadden, Dellagostin and McBride.) |
References: | J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) Front Immunol. 2017 Mar 09;8:256. (PMID: 28337203) J Gen Microbiol. 1986 Jan;132(1):103-9. (PMID: 3711857) Vaccine. 2007 Aug 14;25(33):6277-86. (PMID: 17629368) J Immunol Res. 2015;2015:156241. (PMID: 26526043) Curr Top Med Chem. 2013;13(20):2629-37. (PMID: 24066888) Appl Microbiol Biotechnol. 2022 Jan;106(1):173-184. (PMID: 34893930) BMC Microbiol. 2016 Oct 14;16(1):241. (PMID: 27737644) Nat Rev Microbiol. 2017 May;15(5):297-307. (PMID: 28260786) Vaccine. 2004 Mar 29;22(11-12):1545-52. (PMID: 15063580) Clin Microbiol Infect. 2010 Jun;16(6):593-9. (PMID: 19778300) BMC Microbiol. 2006 Jun 09;6:51. (PMID: 16762078) Res Vet Sci. 2017 Jun;112:156-160. (PMID: 28391058) Vet Microbiol. 2018 Sep;223:47-50. (PMID: 30173751) Immunol Lett. 2006 Jul 15;106(1):1-7. (PMID: 16765451) Front Immunol. 2020 Jul 21;11:1362. (PMID: 32849496) Mol Syst Biol. 2011 Oct 11;7:539. (PMID: 21988835) PLoS Pathog. 2021 Dec 2;17(12):e1009078. (PMID: 34855918) Nat Commun. 2020 Feb 12;11(1):851. (PMID: 32051408) Cold Spring Harb Perspect Biol. 2010 May;2(5):a000414. (PMID: 20452953) Trends Microbiol. 2016 Mar;24(3):198-208. (PMID: 26711681) J Bacteriol. 2006 Nov;188(22):7893-904. (PMID: 16980464) Elife. 2017 Dec 06;6:. (PMID: 29210669) Adv Immunol. 1989;46:111-47. (PMID: 2528896) Vaccine. 2018 May 3;36(19):2574-2580. (PMID: 29625765) Biochim Biophys Acta. 2002 Oct 11;1565(2):308-17. (PMID: 12409203) Microbes Infect. 2018 Oct - Nov;20(9-10):578-588. (PMID: 29452258) Science. 2000 Mar 10;287(5459):1816-20. (PMID: 10710308) Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W509-12. (PMID: 18463140) PLoS Negl Trop Dis. 2011 Dec;5(12):e1422. (PMID: 22180800) Nat Rev Microbiol. 2012 Dec;10(12):807-13. (PMID: 23154260) J Bacteriol. 1988 Mar;170(3):1063-8. (PMID: 2830227) Front Immunol. 2022 Mar 22;13:810834. (PMID: 35392072) Braz J Med Biol Res. 2004 Aug;37(8):1103-9. (PMID: 15273812) Vaccine. 2001 Jan 8;19(11-12):1526-33. (PMID: 11163678) Sci Rep. 2018 Apr 24;8(1):6447. (PMID: 29691454) BMC Bioinformatics. 2008 Jan 23;9:40. (PMID: 18215316) Vaccine. 2009 Oct 19;27(44):6129-36. (PMID: 19715780) BMC Genomics. 2008 Apr 21;9:181. (PMID: 18423054) Int J Mol Sci. 2017 Jan 14;18(1):. (PMID: 28098813) PLoS Negl Trop Dis. 2021 Mar 11;15(3):e0008970. (PMID: 33705392) PLoS Negl Trop Dis. 2019 May 23;13(5):e0007270. (PMID: 31120895) Front Immunol. 2017 Apr 27;8:463. (PMID: 28496441) Front Microbiol. 2017 Mar 14;8:396. (PMID: 28352257) PLoS One. 2009 Jun 16;4(6):e5940. (PMID: 19529772) Exp Toxicol Pathol. 1998 Jun;50(3):191-8. (PMID: 9681649) Curr Top Microbiol Immunol. 2015;387:223-50. (PMID: 25388137) J Biol Chem. 2004 Jun 11;279(24):25420-9. (PMID: 15044492) Sci Rep. 2015 Jun 08;5:10775. (PMID: 26053859) Cell. 2016 Apr 21;165(3):535-50. (PMID: 27104977) Rev Panam Salud Publica. 2018 Feb 19;41:e131. (PMID: 29466519) Infect Immun. 2015 Dec;83(12):4693-700. (PMID: 26416909) J Bacteriol. 1990 May;172(5):2736-46. (PMID: 2139651) Microb Pathog. 1986 Feb;1(1):43-9. (PMID: 2469929) Acta Trop. 2008 Feb;105(2):176-80. (PMID: 18093568) Nucleic Acids Res. 2012 Jul;40(Web Server issue):W471-7. (PMID: 22570420) PLoS Negl Trop Dis. 2017 Mar 16;11(3):e0005441. (PMID: 28301479) Sci Rep. 2018 May 2;8(1):6935. (PMID: 29720698) Hum Vaccin Immunother. 2013 Apr;9(4):763-5. (PMID: 23295984) Infect Immun. 1999 Dec;67(12):6572-82. (PMID: 10569777) J Biotechnol. 2000 Jan 21;76(2-3):97-119. (PMID: 10656326) Front Immunol. 2017 Oct 05;8:1227. (PMID: 29051759) J Infect Dis. 2011 Mar 15;203(6):870-9. (PMID: 21220775) PLoS Pathog. 2019 May 20;15(5):e1007811. (PMID: 31107928) Biophys J. 2016 Feb 23;110(4):930-8. (PMID: 26910429) Vaccine. 2017 Oct 9;35(42):5559-5567. (PMID: 28882437) Curr Top Microbiol Immunol. 2015;387:251-72. (PMID: 25388138) Infect Immun. 2008 Jan;76(1):56-70. (PMID: 17938216) Sci Rep. 2018 Oct 11;8(1):15170. (PMID: 30310115) Bio Protoc. 2015 Jul 20;5(14):. (PMID: 26258158) BMC Microbiol. 2014 May 21;14:132. (PMID: 24884439) Bio Protoc. 2017 May 05;7(9):e2250. (PMID: 34541240) Drug Discov Today. 2021 Sep;26(9):2152-2158. (PMID: 33798647) PLoS Pathog. 2014 Mar 13;10(3):e1004004. (PMID: 24626166) Infect Immun. 2011 Jul;79(7):2936-40. (PMID: 21576342) Expert Opin Drug Discov. 2020 Feb;15(2):179-188. (PMID: 31777290) Elife. 2021 Jan 26;10:. (PMID: 33496263) Vaccine. 2020 Apr 3;38(16):3280-3291. (PMID: 32151463) PLoS Negl Trop Dis. 2015 Sep 17;9(9):e0003898. (PMID: 26379143) Subcell Biochem. 2019;92:9-37. (PMID: 31214983) Sci Rep. 2019 May 2;9(1):6843. (PMID: 31048732) Curr Protoc Microbiol. 2006 Sep;Chapter 12:Unit 12E.2. (PMID: 18770576) Bioinformatics. 2001 Sep;17(9):847-8. (PMID: 11590104) Microbiology (Reading). 2014 Feb;160(Pt 2):429-438. (PMID: 24275101) Vaccine. 2012 May 30;30 Suppl 2:B87-97. (PMID: 22607904) Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. (PMID: 9254694) Front Immunol. 2017 Feb 21;8:58. (PMID: 28270811) Drug Discov Today. 2011 Jul;16(13-14):569-82. (PMID: 21570475) Vaccine. 2009 Nov 5;27(47):6537-45. (PMID: 19729088) Int J Infect Dis. 2017 Apr;57:61-69. (PMID: 28161462) Front Cell Infect Microbiol. 2021 Jun 24;11:677999. (PMID: 34249775) PLoS Negl Trop Dis. 2017 Aug 25;11(8):e0005870. (PMID: 28841659) Curr Opin Infect Dis. 2005 Oct;18(5):376-86. (PMID: 16148523) Biologicals. 2013 Sep;41(5):298-302. (PMID: 23838570) Vaccine. 2010 Jul 5;28(30):4810-6. (PMID: 20483197) Ann N Y Acad Sci. 2020 Feb;1462(1):14-26. (PMID: 31659752) J Med Microbiol. 2010 Sep;59(Pt 9):1101-1106. (PMID: 20558584) Vet Microbiol. 2010 Jan 27;140(3-4):287-96. (PMID: 19345023) J Exp Med. 2016 Apr 4;213(4):469-81. (PMID: 27022144) Clin Vaccine Immunol. 2015 Aug;22(8):965-73. (PMID: 26108285) J Innate Immun. 2018;10(5-6):455-464. (PMID: 30149378) |
فهرسة مساهمة: | Keywords: Leptospira interrogans; animal model; cell-surface immunoprecipitation; outer membrane proteins; reverse and structural vaccinology |
المشرفين على المادة: | 5QB0T2IUN0 (Aluminum Hydroxide) 0 (Bacterial Vaccines) 0 (Antigens, Bacterial) 0 (Recombinant Proteins) 0 (Immunoglobulin G) 0 (Epitopes) |
تواريخ الأحداث: | Date Created: 20221024 Date Completed: 20221025 Latest Revision: 20221222 |
رمز التحديث: | 20221222 |
مُعرف محوري في PubMed: | PMC9586249 |
DOI: | 10.3389/fcimb.2022.940966 |
PMID: | 36275031 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2235-2988 |
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DOI: | 10.3389/fcimb.2022.940966 |