دورية أكاديمية
أعرض في EDS

cAMP-specific phosphodiesterase 8A and 8B isoforms are differentially expressed in human testis and Leydig cell tumor.

التفاصيل البيبلوغرافية
العنوان: cAMP-specific phosphodiesterase 8A and 8B isoforms are differentially expressed in human testis and Leydig cell tumor.
المؤلفون: Campolo F; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Capponi C; Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy., Tarsitano MG; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.; Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, Catanzaro, Italy., Tenuta M; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Pozza C; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Gianfrilli D; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Magliocca F; Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Rome, Italy., Venneri MA; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Vicini E; Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy., Lenzi A; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Isidori AM; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy., Barbagallo F; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.; Faculty of Medicine and Surgery, Kore University of Enna, Enna, Italy.
المصدر: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Oct 07; Vol. 13, pp. 1010924. Date of Electronic Publication: 2022 Oct 07 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: 3',5'-Cyclic-AMP Phosphodiesterases*/genetics , 3',5'-Cyclic-AMP Phosphodiesterases*/metabolism , Leydig Cell Tumor*/genetics, Humans ; Male ; Adenosine Monophosphate ; Protein Isoforms ; Semen
مستخلص: Cyclic adenosine monophosphate/Protein kinase A (cAMP/PKA) signaling pathway is the master regulator of endocrine tissue function. The level, compartmentalization and amplitude of cAMP response are finely regulated by phosphodiesterases (PDEs). PDE8 is responsible of cAMP hydrolysis and its expression has been characterized in all steroidogenic cell types in rodents including adrenal and Leydig cells in rodents however scarce data are currently available in humans. Here we demonstrate that human Leydig cells express both PDE8A and PDE8B isoforms. Interestingly, we found that the expression of PDE8B but not of PDE8A is increased in transformed Leydig cells (Leydig cell tumors-LCTs) compared to non-tumoral cells. Immunofluorescence analyses further reveals that PDE8A is also highly expressed in specific spermatogenic stages. While the protein is not detected in spermatogonia it accumulates nearby the forming acrosome, in the trans-Golgi apparatus of spermatocytes and spermatids and it follows the fate of this organelle in the later stages translocating to the caudal part of the cell. Taken together our findings suggest that 1) a specific pool(s) of cAMP is/are regulated by PDE8A during spermiogenesis pointing out a possible new role of this PDE8 isoform in key events governing the differentiation and maturation of human sperm and 2) PDE8B can be involved in Leydig cell transformation.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Campolo, Capponi, Tarsitano, Tenuta, Pozza, Gianfrilli, Magliocca, Venneri, Vicini, Lenzi, Isidori and Barbagallo.)
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فهرسة مساهمة: Keywords: Leydig cell; Leydig cell tumor; PDE8A; PDE8B; acrosome biogenesis; human testis
المشرفين على المادة: EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases)
415SHH325A (Adenosine Monophosphate)
0 (Protein Isoforms)
EC 3.1.4.17 (PDE8A protein, human)
EC 3.1.4.17 (PDE8B protein, human)
تواريخ الأحداث: Date Created: 20221024 Date Completed: 20221027 Latest Revision: 20221027
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9585345
DOI: 10.3389/fendo.2022.1010924
PMID: 36277728
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-2392
DOI:10.3389/fendo.2022.1010924