دورية أكاديمية
SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis .
| العنوان: | SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis . |
|---|---|
| المؤلفون: | Kathare PK; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712.; The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712., Xin R; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712.; The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712., Ganesan AS; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712.; The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712., June VM; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712.; The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712., Reddy ASN; Department of Biology, Program in Cell and Molecular Plant Biology, Colorado State University, Fort Collins, CO 80523., Huq E; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712.; The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Nov; Vol. 119 (44), pp. e2214565119. Date of Electronic Publication: 2022 Oct 25. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Arabidopsis*/metabolism , Arabidopsis Proteins*/genetics , Arabidopsis Proteins*/metabolism , Phytochrome*/genetics , Phytochrome*/metabolism, Phytochrome B/genetics ; Phytochrome B/metabolism ; RNA Precursors/genetics ; RNA Precursors/metabolism ; RNA Splicing Factors/genetics ; RNA Splicing Factors/metabolism ; Light ; RNA, Messenger/metabolism ; Gene Expression Regulation, Plant ; Mutation |
| مستخلص: | Light signals perceived by a group of photoreceptors have profound effects on the physiology, growth, and development of plants. The red/far-red light-absorbing phytochromes (phys) modulate these aspects by intricately regulating gene expression at multiple levels. Here, we report the identification and functional characterization of an RNA-binding splicing factor, SWAP1 (SUPPRESSOR-OF-WHITE-APRICOT/SURP RNA-BINDING DOMAIN-CONTAINING PROTEIN1). Loss-of-function swap1-1 mutant is hyposensitive to red light and exhibits a day length-independent early flowering phenotype. SWAP1 physically interacts with two other splicing factors, (SFPS) SPLICING FACTOR FOR PHYTOCHROME SIGNALING and (RRC1) REDUCED RED LIGHT RESPONSES IN CRY1CRY2 BACKGROUND 1 in a light-independent manner and forms a ternary complex. In addition, SWAP1 physically interacts with photoactivated phyB and colocalizes with nuclear phyB photobodies. Phenotypic analyses show that the swap1sfps , swap1rrc1, and sfpsrrc1 double mutants display hypocotyl lengths similar to that of the respective single mutants under red light, suggesting that they function in the same genetic pathway. The swap1sfps double and swap1sfpsrrc1 triple mutants display pleiotropic phenotypes, including sterility at the adult stage. Deep RNA sequencing (RNA-seq) analyses show that SWAP1 regulates the gene expression and pre-messenger RNA (mRNA) alternative splicing of a large number of genes, including those involved in plant responses to light signaling. A comparative analysis of alternative splicing among single, double, and triple mutants showed that all three splicing factors coordinately regulate the alternative splicing of a subset of genes. Our study uncovered the function of a splicing factor that modulates light-regulated alternative splicing by interacting with photoactivated phyB and other splicing factors. |
| References: | Annu Rev Biochem. 2020 Jun 20;89:359-388. (PMID: 31794245) Mol Syst Biol. 2008;4:193. (PMID: 18463617) Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1335-40. (PMID: 22232680) Plant Cell. 2019 Sep;31(9):2052-2069. (PMID: 31266850) Mol Cell. 2022 Aug 18;82(16):2916-2918. (PMID: 35985299) Nat Plants. 2021 Oct;7(10):1326-1327. (PMID: 34650266) Plant Physiol. 2012 Jan;158(1):52-60. (PMID: 21951469) Annu Rev Plant Biol. 2021 Jun 17;72:217-244. (PMID: 33756095) Mol Cell. 2019 Mar 7;73(5):1066-1074.e3. (PMID: 30661982) Plant Cell. 2012 Aug;24(8):3278-95. (PMID: 22942380) Plant Cell Physiol. 2018 Jun 1;59(6):1104-1110. (PMID: 29727006) Plant Physiol. 2020 Jan;182(1):243-254. (PMID: 31501299) Plant Cell. 2016 Nov;28(11):2715-2734. (PMID: 27803310) Curr Opin Plant Biol. 2021 Oct;63:102037. (PMID: 33823333) Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):9382-7. (PMID: 26170331) Nat Commun. 2019 Nov 19;10(1):5219. (PMID: 31745087) Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18781-6. (PMID: 25512548) Science. 2014 Apr 25;344(6182):427-30. (PMID: 24763593) Plant Cell. 2019 Oct;31(10):2510-2524. (PMID: 31409629) Plant J. 2012 Nov;72(4):537-46. (PMID: 22849408) Plant Mol Biol. 1994 Jun;25(3):413-27. (PMID: 8049367) Semin Cell Dev Biol. 2019 Aug;92:114-121. (PMID: 30946988) Plant Cell. 2010 Sep;22(9):2926-9. (PMID: 20884799) Front Plant Sci. 2019 Jun 12;10:708. (PMID: 31244866) Elife. 2021 Mar 30;10:. (PMID: 33783355) Plant Cell. 2018 Jun;30(6):1277-1292. (PMID: 29764986) Nat Rev Genet. 2014 Oct;15(10):689-701. (PMID: 25112293) Genome Biol. 2022 Jul 7;23(1):149. (PMID: 35799267) Nat Commun. 2021 Sep 23;12(1):5614. (PMID: 34556672) Annu Rev Biochem. 2015;84:291-323. (PMID: 25784052) Mol Cell. 2022 Aug 18;82(16):3015-3029.e6. (PMID: 35728588) Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2214565119. (PMID: 36282917) Nat Plants. 2021 Oct;7(10):1397-1408. (PMID: 34650267) Annu Rev Plant Biol. 2014;65:311-33. (PMID: 24779996) Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):E7018-E7027. (PMID: 28760995) Bioinformatics. 2021 Jun 10;:. (PMID: 34109387) Plant Physiol. 2010 Jul;153(3):1161-74. (PMID: 20466843) Plant J. 2012 Jun;70(5):727-38. (PMID: 22324426) Nature. 2010 Nov 4;468(7320):112-6. (PMID: 20962777) Plant Cell. 2012 Oct;24(10):4066-82. (PMID: 23110899) Front Plant Sci. 2022 Feb 10;13:836519. (PMID: 35222493) |
| فهرسة مساهمة: | Keywords: Arabidopsis; photomorphogenesis; phytochrome B; pre-mRNA splicing; splicing factor |
| المشرفين على المادة: | 136250-22-1 (Phytochrome B) 0 (Arabidopsis Proteins) 0 (RNA Precursors) 0 (RNA Splicing Factors) 11121-56-5 (Phytochrome) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20221025 Date Completed: 20221027 Latest Revision: 20221108 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9636961 |
| DOI: | 10.1073/pnas.2214565119 |
| PMID: | 36282917 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
|---|---|
| DOI: | 10.1073/pnas.2214565119 |