دورية أكاديمية
أعرض في EDS

Effectiveness of antimicrobial photodynamic therapy mediated by butyl toluidine blue in preventing medication-related osteonecrosis of the jaws in rats.

التفاصيل البيبلوغرافية
العنوان: Effectiveness of antimicrobial photodynamic therapy mediated by butyl toluidine blue in preventing medication-related osteonecrosis of the jaws in rats.
المؤلفون: Ervolino E; Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil. Electronic address: e.ervolino@unesp.br., Olivo MB; Department of Diagnostic and Surgery, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil., Toro LF; Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil; Institute of Biosciences, São Paulo State University (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250, Botucatu, SP 18618-689, Brazil., Freire JOA; Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil; Institute of Biosciences, São Paulo State University (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250, Botucatu, SP 18618-689, Brazil., Ganzaroli VF; Department of Diagnostic and Surgery, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil., Guiati IZ; Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil; Institute of Biosciences, São Paulo State University (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250, Botucatu, SP 18618-689, Brazil., Nuernberg MAA; Department of Diagnostic and Surgery, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil., Franciscon JPS; Department of Diagnostic and Surgery, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil., Ângelo Cintra LT; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil., Garcia VG; Latin American Institute of Dental Research and Education (ILAPEO), R. Jacarezinho, 656, Curitiba, PR 80710-150, Brazil., Wainwright M; School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom., Theodoro LH; Department of Diagnostic and Surgery, School of Dentistry, São Paulo State University (UNESP), Rod. Marechal Rondon, Km 528, Araçatuba, SP 16015-050, Brazil.
المصدر: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2022 Dec; Vol. 40, pp. 103172. Date of Electronic Publication: 2022 Oct 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101226123 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-1597 (Electronic) Linking ISSN: 15721000 NLM ISO Abbreviation: Photodiagnosis Photodyn Ther Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; Boston : Elsevier, c2004-
مواضيع طبية MeSH: Photochemotherapy*/methods , Anti-Infective Agents*, Rats ; Female ; Animals ; Tolonium Chloride/pharmacology ; Zoledronic Acid/pharmacology ; Molar/surgery ; Molar/pathology
مستخلص: Background: Medication-related osteonecrosis of the jaws (MRONJ) is difficult to treat, therefore, prevention would be the ideal clinical approach. This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT), mediated by butyl toluidine blue (BuTB) in the prevention of MRONJ-like lesions after tooth extraction in rats.
Methods: Twenty-eight senescent female rats were distributed in groups: VEH and VEH-aPDT, treated with vehicle, ZOL and ZOL-aPDT, treated with 100 µg/Kg of zoledronate, both treatments every three days over seven weeks. After three weeks from the commencement of treatment, the mandibular first molar was extracted. For the VEH and ZOL groups, no local treatment was performed, while with the VEH-aPDT and ZOL-aPDT groups, photodynamic treatment was carried out at 0, 2, and 4 days after extraction. For aPDT, 500μl of BuTB solution was deposited on the dental extraction site (0.5 mg/ml; 60 s), followed by irradiation with low-level laser (InGaAIP; 660 nm; 35 mW; 74.2 J/cm²; 60 s). After 28 postoperative days, euthanasia was performed. The hemimandibles were processed to: (1) histological analysis of tissue repair; (2) histometric analysis of the percentage of newly formed bone tissue (PNFBT) and percentage of non-vital bone tissue (PNVBT); (3) immunohistochemical analysis for tartrate-resistant acid phosphatase (TRAP).
Results: The ZOL and ZOL-aPDT groups showed less TRAP-positive cells when compared with VEH and VEH-aPDT. The ZOL group demonstrated great compromise in the tissue repair process, consistent with MRONJ-like lesions. VEH, VEH-aPDT and ZOL-aPDT presented a favorable tissue repair process. PNFBT in the ZOL group was lower than in the VEH, VEH-aPDT and ZOL-aPDT groups, whereas PNVBT in the ZOL group was higher than in the VEH, VEH-aPDT and ZOL-aPDT groups.
Conclusion: aPDT mediated by BuTB prevented the occurrence of MRONJ-like lesions after tooth extraction in rats.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Bisphosphonates; Butyl toluidine blue; Jaws; Osteonecrosis; Photodynamic therapy; Prevention
المشرفين على المادة: 15XUH0X66N (Tolonium Chloride)
6XC1PAD3KF (Zoledronic Acid)
0 (Anti-Infective Agents)
تواريخ الأحداث: Date Created: 20221025 Date Completed: 20221216 Latest Revision: 20221220
رمز التحديث: 20240628
DOI: 10.1016/j.pdpdt.2022.103172
PMID: 36283616
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-1597
DOI:10.1016/j.pdpdt.2022.103172