دورية أكاديمية
أعرض في EDS

Acetylenotrophic and Diazotrophic Bradyrhizobium sp. Strain I71 from TCE-Contaminated Soils.

التفاصيل البيبلوغرافية
العنوان: Acetylenotrophic and Diazotrophic Bradyrhizobium sp. Strain I71 from TCE-Contaminated Soils.
المؤلفون: Akob DM; U.S. Geological Survey, Geology, Energy & Minerals Science Center, Reston, Virginia, USA., Sutton JM; Department of Biological Sciences, The University of Alabama, Tuscaloosagrid.411015.0, Alabama, USA., Bushman TJ; U.S. Geological Survey, Geology, Energy & Minerals Science Center, Reston, Virginia, USA.; Department of Biological Sciences, The University of Alabama, Tuscaloosagrid.411015.0, Alabama, USA., Baesman SM; U.S. Geological Survey, Menlo Park, California, USA., Klein E; Institute for Applied Biology, Department of Applied Biology, Karlsruhe Institute of Technologygrid.7892.4, Karlsruhe, Germany., Shrestha Y; U.S. Geological Survey, Geology, Energy & Minerals Science Center, Reston, Virginia, USA., Andrews R; U.S. Geological Survey, Geology, Energy & Minerals Science Center, Reston, Virginia, USA., Fierst JL; Biomolecular Sciences Institute, Department of Biological Sciences, Florida International University, Miami, Florida, USA., Kolton M; French Associates Institute for Agriculture and Biotechnology of Drylands, Ben-Gurion University of the Negev, Beer Sheva, Israel.; School of Biological Sciences, Georgia Institute of Technologygrid.213917.f, Atlanta, Georgia, USA., Gushgari-Doyle S; Department of Civil and Environmental Engineering, University of California, Berkeley, California, USA., Oremland RS; U.S. Geological Survey, Menlo Park, California, USA., Freeman JL; Intrinsyx Environmental, Valley Research Park, Mountain View, California, USA.
المصدر: Applied and environmental microbiology [Appl Environ Microbiol] 2022 Nov 22; Vol. 88 (22), pp. e0121922. Date of Electronic Publication: 2022 Oct 26.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Society for Microbiology.
مواضيع طبية MeSH: Bradyrhizobium* , Trichloroethylene*/metabolism, Nitrogen Fixation/genetics ; Soil/chemistry ; Acetylene/metabolism ; Phylogeny ; Symbiosis ; RNA, Ribosomal, 16S/genetics ; Root Nodules, Plant/microbiology ; DNA, Bacterial/genetics ; Sequence Analysis, DNA
مستخلص: Acetylene (C 2 H 2 ) is a molecule rarely found in nature, with very few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs; however, we hypothesize there may yet be unrecognized diversity of acetylenotrophs in nature. This study expands the known diversity of acetylenotrophs by isolating the aerobic acetylenotroph, Bradyrhizobium sp. strain I71, from trichloroethylene (TCE)-contaminated soils. Strain I71 is a member of the class Alphaproteobacteria and exhibits acetylenotrophic and diazotrophic activities, the only two enzymatic reactions known to transform acetylene. This unique capability in the isolated strain may increase the genus' economic impact beyond agriculture as acetylenotrophy is closely linked to bioremediation of chlorinated contaminants. Computational analyses indicate that the Bradyrhizobium sp. strain I71 genome contains 522 unique genes compared to close relatives. Moreover, applying a novel hidden Markov model of known acetylene hydratase (AH) enzymes identified a putative AH enzyme. Protein annotation with I-TASSER software predicted the AH from the microbe Syntrophotalea acetylenica as the closest structural and functional analog. Furthermore, the putative AH was flanked by horizontal gene transfer (HGT) elements, like that of AH in anaerobic acetylenotrophs, suggesting an unknown source of acetylene or acetylenic substrate in the environment that is selecting for the presence of AH. IMPORTANCE The isolation of Bradyrhizobium strain I71 expands the distribution of acetylene-consuming microbes to include a group of economically important microorganisms. Members of Bradyrhizobium are well studied for their abilities to improve plant health and increase crop yields by providing bioavailable nitrogen. Additionally, acetylene-consuming microbes have been shown to work in tandem with other microbes to degrade soil contaminants. Based on genome, cultivation, and protein prediction analysis, the ability to consume acetylene is likely not widespread within the genus Bradyrhizobium . These findings suggest that the suite of phenotypic capabilities of strain I71 may be unique and make it a good candidate for further study in several research avenues.
References: Front Microbiol. 2019 Mar 12;10:407. (PMID: 30915047)
Int J Syst Evol Microbiol. 2020 Nov;70(11):5972-6016. (PMID: 33151140)
Nat Protoc. 2010 Apr;5(4):725-38. (PMID: 20360767)
Int J Syst Evol Microbiol. 2019 Jun;71(3):. (PMID: 33570486)
DNA Res. 2002 Dec 31;9(6):189-97. (PMID: 12597275)
J Bacteriol. 2001 Feb;183(4):1405-12. (PMID: 11157954)
Nucleic Acids Res. 2021 Jan 8;49(D1):D545-D551. (PMID: 33125081)
Met Ions Life Sci. 2014;14:15-35. (PMID: 25416389)
PeerJ. 2015 Oct 08;3:e1319. (PMID: 26500826)
BMC Genomics. 2012 May 03;13:162. (PMID: 22554235)
Environ Sci Technol. 2017 Feb 21;51(4):2366-2372. (PMID: 28075122)
Int J Syst Evol Microbiol. 2016 Feb;66(2):1100-1103. (PMID: 26585518)
Proc Natl Acad Sci U S A. 1967 Nov;58(5):2071-8. (PMID: 5237501)
J Bacteriol. 1995 Oct;177(20):5767-72. (PMID: 7592321)
Nucleic Acids Res. 2020 Jan 8;48(D1):D265-D268. (PMID: 31777944)
Appl Environ Microbiol. 2017 Aug 17;83(17):. (PMID: 28667112)
Nucleic Acids Res. 2016 Aug 19;44(14):6614-24. (PMID: 27342282)
FEMS Microbiol Lett. 2017 May 1;364(10):. (PMID: 28379342)
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3073-7. (PMID: 17360611)
Appl Environ Microbiol. 2007 Apr;73(8):2635-43. (PMID: 17308185)
Appl Environ Microbiol. 1977 May;33(5):1225-8. (PMID: 327932)
Bioinformatics. 2013 Nov 1;29(21):2669-77. (PMID: 23990416)
Mol Biol Evol. 2021 Dec 9;38(12):5825-5829. (PMID: 34597405)
Nucleic Acids Res. 2014 Jan;42(Database issue):D26-31. (PMID: 24225321)
Water Res. 2012 Dec 1;46(19):6339-50. (PMID: 23039917)
Int J Syst Bacteriol. 1994 Jul;44(3):392-403. (PMID: 7520731)
FEMS Microbiol Ecol. 2018 Aug 1;94(8):. (PMID: 29933435)
Nucleic Acids Res. 2016 Jan 4;44(D1):D7-19. (PMID: 26615191)
Appl Environ Microbiol. 2022 Nov 22;88(22):e0121922. (PMID: 36286524)
Biochem J. 1976 Aug 1;157(2):495-7. (PMID: 962879)
Nat Methods. 2015 Jan;12(1):7-8. (PMID: 25549265)
FEMS Microbiol Lett. 1997 Mar 15;148(2):175-80. (PMID: 9084145)
PeerJ. 2018 Jan 25;6:e4320. (PMID: 29423345)
Environ Sci Technol. 2017 Sep 5;51(17):10050-10058. (PMID: 28737929)
FEMS Microbiol Ecol. 2003 May 1;44(2):191-202. (PMID: 19719636)
Genome Announc. 2017 Feb 9;5(6):. (PMID: 28183760)
Chemosphere. 2015 Jan;119:744-749. (PMID: 25192648)
Front Microbiol. 2019 Jun 13;10:1334. (PMID: 31263459)
Mol Biol Evol. 2020 May 1;37(5):1530-1534. (PMID: 32011700)
Syst Appl Microbiol. 2019 Jul;42(4):427-439. (PMID: 31031014)
J Microbiol. 2018 Apr;56(4):280-285. (PMID: 29492869)
Can J Microbiol. 1988 Mar;34(3):242-8. (PMID: 3416237)
Nucleic Acids Res. 2015 Jul 1;43(W1):W174-81. (PMID: 25883148)
Mol Biol Evol. 2018 Feb 1;35(2):518-522. (PMID: 29077904)
J Bacteriol. 1982 May;150(2):989-92. (PMID: 6121789)
Mol Biol Evol. 2015 Jan;32(1):268-74. (PMID: 25371430)
J Mol Biol. 2016 Feb 22;428(4):726-731. (PMID: 26585406)
Appl Environ Microbiol. 2017 Aug 17;83(17):. (PMID: 28667109)
Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):81-91. (PMID: 17220447)
Biochim Biophys Acta. 1966 Oct 31;127(2):285-94. (PMID: 5964974)
Syst Appl Microbiol. 2020 May;43(3):126073. (PMID: 32139173)
Front Microbiol. 2018 Jul 24;9:1644. (PMID: 30087663)
Appl Environ Microbiol. 2012 May;78(9):3504-7. (PMID: 22367087)
Genome Announc. 2017 Feb 9;5(6):. (PMID: 28183759)
PLoS One. 2014 Nov 19;9(11):e112963. (PMID: 25409509)
ISME J. 2017 Nov;11(11):2399-2406. (PMID: 28731467)
Appl Microbiol. 1975 Oct;30(4):707-9. (PMID: 1190767)
J Bacteriol. 2011 Mar;193(5):1229-36. (PMID: 21193613)
Proc Natl Acad Sci U S A. 1967 Jul;58(1):213-6. (PMID: 5231601)
Environ Sci Technol. 2003 Jul 15;37(14):3181-8. (PMID: 12901668)
Astrobiology. 2015 Nov;15(11):977-86. (PMID: 26539733)
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7. (PMID: 15034147)
Mol Biol Evol. 2009 Jul;26(7):1641-50. (PMID: 19377059)
ISME J. 2015 Nov;9(11):2435-41. (PMID: 25909973)
Nucleic Acids Res. 2019 Jan 8;47(D1):D309-D314. (PMID: 30418610)
mBio. 2021 Feb 2;12(1):. (PMID: 33531396)
فهرسة مساهمة: Keywords: Bradyrhizobium; acetylene; acetylenotrophy; microbial ecology; nitrogen fixation; soil microbiology
المشرفين على المادة: 290YE8AR51 (Trichloroethylene)
0 (Soil)
OC7TV75O83 (Acetylene)
0 (RNA, Ribosomal, 16S)
0 (DNA, Bacterial)
تواريخ الأحداث: Date Created: 20221026 Date Completed: 20221124 Latest Revision: 20230427
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9680620
DOI: 10.1128/aem.01219-22
PMID: 36286524
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5336
DOI:10.1128/aem.01219-22