دورية أكاديمية
Genetic and phenotypical diversity of Pseudomonas syringae population in the Russian Federation.
| العنوان: | Genetic and phenotypical diversity of Pseudomonas syringae population in the Russian Federation. |
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| المؤلفون: | Tarakanov RI; Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow, Russia., Ignatov AN; People's Friendship University of Russia - RUDN University, Agrarian Technological Institute, Moscow, Russia., Dzhalilov FS; Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow, Russia. |
| المصدر: | Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2022 Oct 21; Vol. 84, pp. e264224. Date of Electronic Publication: 2022 Oct 21 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Ecology Country of Publication: Brazil NLM ID: 101129542 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-4375 (Electronic) Linking ISSN: 15196984 NLM ISO Abbreviation: Braz J Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: São Carlos, SP [Brazil] : International Institute of Ecology, [2000]- |
| مواضيع طبية MeSH: | Plant Diseases* , Pseudomonas syringae*/genetics, Anti-Bacterial Agents ; Citrate (si)-Synthase ; DNA-Directed RNA Polymerases/genetics ; Glycine max ; Russia |
| مستخلص: | Proteobacteria comprising species of Pseudomonas syringae group cause diseases of many plants around the world. The phytopathogen has a complex taxonomic structure, which is constantly being revised due to the emergence of new molecular and biochemical diagnostic methods. Here for the first time, we describe the genetic and phenotypic diversity of 57 strains of Pseudomonas syringae isolated from affected soybeans, cereals, sunflowers, and other plants in the Russian Federation from 1950 to 2019. Genetic diversity was assessed by Multi Locus Sequence Analysis (MLSA) using fragments of the genes of glyceraldehyde-3-phosphate dehydrogenase (gapdh), the DNA-directed RNA polymerase subunit D (rpoD), gyrase (topoisomerase) B subunit (gyrB), and citrate synthase I (gltA). The synthesis of syringomycin and coronatine by bacteria was assessed by the reaction of susceptible yeast culture, seedlings of barley, tomato, and sunflower, and by presence of toxin genes confirmed by PCR test. The pathogenicity of the strains was confirmed on seedlings of dicotyledonous and monocotyledonous plants of peas, soybean, sunflowers, barley and wheat, as the most affected crops. The sensitivity of bacteria to 10 antibiotics of the main mechanisms of activity and two bactericidal commercial products was tested by standard disc method. The obtained results showed a high genetic homogeneity of the Russian population of P. syringae, which infects various agricultural crops, and an increase in the proportion of antibiotic-resistant strains over the years. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) EC 2.3.3.1 (Citrate (si)-Synthase) EC 2.7.7.6 (DNA-Directed RNA Polymerases) |
| تواريخ الأحداث: | Date Created: 20221026 Date Completed: 20221031 Latest Revision: 20231213 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/1519-6984.264224 |
| PMID: | 36287527 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-4375 |
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| DOI: | 10.1590/1519-6984.264224 |