دورية أكاديمية
أعرض في EDS

Collagen V α1 Chain Decrease in Papillary Dermis from Early Systemic Sclerosis: A New Proposal in Cutaneous Fibrosis Molecular Structure.

التفاصيل البيبلوغرافية
العنوان: Collagen V α1 Chain Decrease in Papillary Dermis from Early Systemic Sclerosis: A New Proposal in Cutaneous Fibrosis Molecular Structure.
المؤلفون: de Morais J; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Velosa APP; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Andrade PC; Department of Pathology, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Frediani D; Discipline of Clinical Emergency (LIM 51), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Carrasco S; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Queiroz ZAJ; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Martin P; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Saito RF; Center for Translational Research in Oncology (LIM24), Department of Radiology and Oncology, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.; Instituto do Câncer do Estado de São Paulo, São Paulo 01246-903, Brazil., Elias V; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Goldenstein-Schainberg C; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Chammas R; Center for Translational Research in Oncology (LIM24), Department of Radiology and Oncology, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.; Instituto do Câncer do Estado de São Paulo, São Paulo 01246-903, Brazil., Sampaio-Barros PD; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Capelozzi VL; Department of Pathology, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil., Teodoro WR; Division of Rheumatology (LIM 17), Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2022 Oct 21; Vol. 23 (20). Date of Electronic Publication: 2022 Oct 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Dermis*/metabolism , Scleroderma, Systemic*/pathology, Humans ; RNA, Small Interfering/metabolism ; Molecular Structure ; Fibrosis ; Collagen/metabolism ; Skin/metabolism ; Fibroblasts/metabolism
مستخلص: Cutaneous fibrosis is one of the main features of systemic sclerosis (SSc). Recent findings correlated abnormal collagen V (Col V) deposition in dermis with skin thickening and disease activity in SSc. Considering that Col V is an important regulator of collagen fibrillogenesis, understanding the role of Col V in the first two years of the skin fibrosis in SSc (early SSc) can help to determine new targets for future treatments. In this study, we analyzed the morphological, ultrastructural and molecular features of α1(V) and α2(V) chains and the expression of their coding genes COL5A1 and COL5A2 in collagen fibrillogenesis in early-SSc. Skin biopsies were obtained from seven consecutive treatment-naïve patients with SSc-related fibrosis and four healthy controls. Our data showed increased α1(V) and α2(V) chain expression in the reticular dermis of early-SSc patients; however, immunofluorescence and ultrastructural immunogold staining determined a significant decreased expression of the α1(V) chain along the dermoepidermal junction in the papillary dermis from early-SSc-patients in relation to the control (12.77 ± 1.34 vs. 66.84 ± 3.36; p < 0.0001). The immunoblot confirmed the decreased expression of the α1(V) chain by the cutaneous fibroblasts of early-SSc, despite the increased COL5A1 and COL5A2 gene expression. In contrast, the α2(V) chain was overexpressed in the small vessels (63.18 ± 3.56 vs. 12.16 ± 0.81; p < 0.0001) and capillaries (60.88 ± 5.82 vs. 15.11 ± 3.80; p < 0.0001) in the reticular dermis of early-SSc patients. Furthermore, COLVA2 siRNA in SSc cutaneous fibroblasts resulted in a decreased α1(V) chain expression. These results highlight an intense decrease in the α1(V) chain along the dermoepidermal junction, suggesting an altered molecular histoarchitecture in the SSc papillary dermis, with a possible decrease in the expression of the α1(V)3 homotrimeric isoform, which could interfere with the thickening and cutaneous fibrosis related to SSc.
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معلومات مُعتمدة: 2018/00415-0; 2018/20403-6; 2021/13220-5 São Paulo Research Foundation
فهرسة مساهمة: Keywords: collagen V alpha chains; fibrosis; papillary dermis; skin; systemic sclerosis
المشرفين على المادة: 0 (RNA, Small Interfering)
9007-34-5 (Collagen)
تواريخ الأحداث: Date Created: 20221027 Date Completed: 20221028 Latest Revision: 20230308
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9604101
DOI: 10.3390/ijms232012654
PMID: 36293511
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms232012654