دورية أكاديمية
Ginkgo biloba Extract Stimulates Adipogenesis in 3T3-L1 Preadipocytes.
| العنوان: | Ginkgo biloba Extract Stimulates Adipogenesis in 3T3-L1 Preadipocytes. |
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| المؤلفون: | Thomaz FM; Post-Graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema 09972-270, Brazil., de Jesus Simão J; Post-Graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema 09972-270, Brazil., da Silva VS; Post-Graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema 09972-270, Brazil., Machado MMF; Post-Graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema 09972-270, Brazil., Oyama LM; Discipline of Nutrition Physiology, Department of Physiology, Universidade Federal de São Paulo-UNIFESP, São Paulo 04023-062, Brazil., Ribeiro EB; Discipline of Nutrition Physiology, Department of Physiology, Universidade Federal de São Paulo-UNIFESP, São Paulo 04023-062, Brazil., Alonso Vale MIC; Post-Graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema 09972-270, Brazil., Telles MM; Post-Graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema 09972-270, Brazil.; Discipline of Nutrition Physiology, Department of Physiology, Universidade Federal de São Paulo-UNIFESP, São Paulo 04023-062, Brazil. |
| المصدر: | Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2022 Oct 20; Vol. 15 (10). Date of Electronic Publication: 2022 Oct 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101238453 Publication Model: Electronic Cited Medium: Print ISSN: 1424-8247 (Print) Linking ISSN: 14248247 NLM ISO Abbreviation: Pharmaceuticals (Basel) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c2004- |
| مستخلص: | Smaller adipocytes are related to the reversal of metabolic disorders, suggesting that molecules that can act in the adipogenesis pathway are of great interest. The objective of this study was to investigate the effect of Ginkgo biloba extract (GbE) in modulating the differentiation in preadipocytes. 3T3-L1 preadipocytes were differentiated for 7 days into adipocytes without (control group) and with GbE at 1.0 mg/mL. Lipid content and gene expression were analyzed on day 7 (D7) by Oil Red O staining and PCR Array Gene Expression. Western blotting analysis of the key adipogenesis markers was evaluated during the differentiation process at days 3 (D3), 5 (D5), and 7 (D7). GbE increased lipid content and raised the gene expression of the main adipogenesis markers. Key proteins of the differentiation process were modulated by GbE, since C/EBPβ levels were decreased, while C/EBPα levels were increased at D7. Regarding the mature adipocytes' markers, GbE enhanced the levels of both FABP4 at D5, and perilipin at D3 and D5. In summary, the present findings showed that GbE modulated the adipogenesis pathway suggesting that the treatment could accelerate the preadipocyte maturation, stimulating the expression of mature adipocyte proteins earlier than expected. |
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| معلومات مُعتمدة: | #2014/18929-9 Fundação de Amparo à Pesquisa do Estado de São Paulo |
| فهرسة مساهمة: | Keywords: adipocytes hyperplasia; natural compounds; polyphenols; preadipocytes maturation; pro-adipogenesis effect |
| تواريخ الأحداث: | Date Created: 20221027 Latest Revision: 20221030 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9610090 |
| DOI: | 10.3390/ph15101294 |
| PMID: | 36297406 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1424-8247 |
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| DOI: | 10.3390/ph15101294 |