دورية أكاديمية
Guidelines for mouse and human DC generation.
| العنوان: | Guidelines for mouse and human DC generation. |
|---|---|
| المؤلفون: | Lutz MB; Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany., Ali S; Institute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, Germany., Audiger C; Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.; Department of Medical Biology, University of Melbourne, Parkville, VIC, 3052, Australia., Autenrieth SE; Dendritic Cells in Infection and Cancer (F171), German Cancer Research Center (DKFZ), Heidelberg, Germany.; Department of Internal Medicine II, University of Tübingen, Tübingen, Germany., Berod L; Institute of Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany., Bigley V; Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, United Kingdom., Cyran L; Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany., Dalod M; CNRS, INSERM, Aix Marseille Univ, Centre d'Immunologie de Marseille-Luminy, Turing Center for Living Systems, Marseille, France., Dörrie J; RNA-based Immunotherapy, Hautklinik, Universitätsklinikum Erlangen (UKER), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany.; Comprehensive Cancer Center Erlangen European Metropolitan Area of Nuremberg (CCC ER-EMN), Östliche Stadtmauerstraße 30, 91054, Erlangen, Germany.; Deutsches Zentrum Immuntherapie (DZI), Ulmenweg 18, 91054, Erlangen, Germany., Dudziak D; Comprehensive Cancer Center Erlangen European Metropolitan Area of Nuremberg (CCC ER-EMN), Östliche Stadtmauerstraße 30, 91054, Erlangen, Germany.; Deutsches Zentrum Immuntherapie (DZI), Ulmenweg 18, 91054, Erlangen, Germany.; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), D-91054, Erlangen, Germany., Flórez-Grau G; Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands., Giusiano L; Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany., Godoy GJ; Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany., Heuer M; Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany., Krug AB; Institute for Immunology, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany., Lehmann CHK; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), D-91054, Erlangen, Germany., Mayer CT; Experimental Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA., Naik SH; Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.; Department of Medical Biology, University of Melbourne, Parkville, VIC, 3052, Australia., Scheu S; Institute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, Germany., Schreibelt G; Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboudumc, Nijmegen, the Netherlands., Segura E; Institut Curie, PSL Research University, INSERM, U932, 26 rue d'Ulm, Paris, 75005, France., Seré K; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany.; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany., Sparwasser T; Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, 55131, Germany., Tel J; Laboratory of Immunoengineering, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands., Xu H; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany.; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany., Zenke M; Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen University Medical School, Aachen, Germany.; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany. |
| المصدر: | European journal of immunology [Eur J Immunol] 2023 Nov; Vol. 53 (11), pp. e2249816. Date of Electronic Publication: 2022 Oct 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4141 (Electronic) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005->: Weinheim : Wiley-VCH Original Publication: Weinheim, Verlag Chemie GmbH. |
| مواضيع طبية MeSH: | Dendritic Cells* , Monocytes*, Animals ; Mice ; Humans ; Antigens, CD34 ; Phenotype ; Cell Differentiation |
| مستخلص: | This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. This article provides protocols with top ticks and pitfalls for preparation and successful generation of mouse and human DC from different cellular sources, such as murine BM and HoxB8 cells, as well as human CD34 + cells from cord blood, BM, and peripheral blood or peripheral blood monocytes. We describe murine cDC1, cDC2, and pDC generation with Flt3L and the generation of BM-derived DC with GM-CSF. Protocols for human DC generation focus on CD34 + cell culture on OP9 cell layers for cDC1, cDC2, cDC3, and pDC subset generation and DC generation from peripheral blood monocytes (MoDC). Additional protocols include enrichment of murine DC subsets, CRISPR/Cas9 editing, and clinical grade human DC generation. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists. (© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.) |
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| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust; MR/W01677X/1 United Kingdom MRC_ Medical Research Council; ZIA BC011975 United States ImNIH Intramural NIH HHS; 101155/Z/13/Z United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: Dendritic cells; Generation; In vitro; Isolation |
| المشرفين على المادة: | 0 (Antigens, CD34) |
| تواريخ الأحداث: | Date Created: 20221028 Date Completed: 20231102 Latest Revision: 20231210 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10704271 |
| DOI: | 10.1002/eji.202249816 |
| PMID: | 36303448 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1521-4141 |
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| DOI: | 10.1002/eji.202249816 |