دورية أكاديمية

Water transport regulates nucleus volume, cell density, Young's modulus, and E-cadherin expression in tumor spheroids.

التفاصيل البيبلوغرافية
العنوان: Water transport regulates nucleus volume, cell density, Young's modulus, and E-cadherin expression in tumor spheroids.
المؤلفون: Conrad C; Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA., Conway J; Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA., Polacheck WJ; Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, USA., Rizvi I; Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, USA., Scarcelli G; Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA. Electronic address: scarc@umd.edu.
المصدر: European journal of cell biology [Eur J Cell Biol] 2022 Sep-Nov; Vol. 101 (4), pp. 151278. Date of Electronic Publication: 2022 Oct 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Germany NLM ID: 7906240 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1298 (Electronic) Linking ISSN: 01719335 NLM ISO Abbreviation: Eur J Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Jena, Germany : Elsevier
Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, <1979-1997>
مواضيع طبية MeSH: Cadherins*/metabolism , Ovarian Neoplasms*/metabolism, Female ; Humans ; Cell Line, Tumor ; Cell Nucleus Size ; Cell Count ; Water
مستخلص: Cell volume is maintained by the balance of water and solutes across the cell membrane and plays an important role in mechanics and biochemical signaling in cells. Here, we assess the relationship between cell volume, mechanical properties, and E-cadherin expression in three-dimensional cultures for ovarian cancer. To determine the effect of water transport in multi-cellular tumors, ovarian cancer spheroids were subjected to hypotonic and hypertonic shock using water and sucrose mixtures, respectively. Increased osmolality resulted in decreased nucleus volume, increased Young's modulus, and increased tumor cell density in ovarian cancer spheroids. Next, we looked at the reversibility of mechanics and morphology after 5 min of osmotic shock and found that spheroids had a robust ability to return to their original state. Finally, we quantified the size of E-cadherin clusters at cell-cell junctions and observed a significant increase in aggregate size following 30 min of hypertonic and hypotonic osmotic shocks. Yet, these effects were not apparent after 5 min of osmotic shock, illustrating a temporal difference between E-cadherin regulation and the immediate mechanical and morphology changes. Still, the osmotically induced E-cadherin aggregates which formed at the 30-minute timepoint was reversible when spheroids were replenished with isotonic medium. Altogether, this work demonstrated an important role of osmolality in transforming mechanical, morphology, and molecular states.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.)
معلومات مُعتمدة: R00 CA175292 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: Cancer; Cell volume; E-cadherin; Mechanics; Osmolality
المشرفين على المادة: 0 (Cadherins)
059QF0KO0R (Water)
تواريخ الأحداث: Date Created: 20221028 Date Completed: 20221216 Latest Revision: 20240611
رمز التحديث: 20240611
DOI: 10.1016/j.ejcb.2022.151278
PMID: 36306595
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-1298
DOI:10.1016/j.ejcb.2022.151278