Severe inbreeding, increased mutation load and gene loss-of-function in the critically endangered Devils Hole pupfish.

التفاصيل البيبلوغرافية
العنوان:	Severe inbreeding, increased mutation load and gene loss-of-function in the critically endangered Devils Hole pupfish.
المؤلفون:	Tian D; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.; Museum of Vertebrate Zoology, University of California, Berkeley, CA 94720, USA., Patton AH; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.; Museum of Vertebrate Zoology, University of California, Berkeley, CA 94720, USA., Turner BJ; Museum of Vertebrate Zoology, University of California, Berkeley, CA 94720, USA., Martin CH; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.; Museum of Vertebrate Zoology, University of California, Berkeley, CA 94720, USA.
المصدر:	Proceedings. Biological sciences [Proc Biol Sci] 2022 Nov 09; Vol. 289 (1986), pp. 20221561. Date of Electronic Publication: 2022 Nov 02.
نوع المنشور:	Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural
اللغة:	English
بيانات الدورية:	Publisher: Royal Society of London Country of Publication: England NLM ID: 101245157 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1471-2954 (Electronic) Linking ISSN: 09628452 NLM ISO Abbreviation: Proc Biol Sci Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: London : Royal Society of London, c1990-
مواضيع طبية MeSH:	Inbreeding* , Killifishes*, Male ; Humans ; Animals ; Sperm Motility ; Endangered Species ; Mutation ; Genetic Variation
مستخلص:	Small populations with limited range are often threatened by inbreeding and reduced genetic diversity, which can reduce fitness and exacerbate population decline. One of the most extreme natural examples is the Devils Hole pupfish ( Cyprinodon diabolis ), an iconic and critically endangered species with the smallest known range of any vertebrate. This species has experienced severe declines in population size over the last 30 years and suffered major bottlenecks in 2007 and 2013, when the population shrunk to 38 and 35 individuals, respectively. Here, we analysed 30 resequenced genomes of desert pupfishes from Death Valley, Ash Meadows and surrounding areas to examine the genomic consequences of small population size. We found extremely high levels of inbreeding ( F ROH = 0.34-0.81) and an increased amount of potentially deleterious genetic variation in the Devils Hole pupfish as compared to other species, including unique, fixed loss-of-function alleles and deletions in genes associated with sperm motility and hypoxia. Additionally, we successfully resequenced a formalin-fixed museum specimen from 1980 and found that the population was already highly inbred prior to recent known bottlenecks. We thus document severe inbreeding and increased mutation load in the Devils Hole pupfish and identify candidate deleterious variants to inform management of this conservation icon.
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معلومات مُعتمدة:	R01 DE027052 United States DE NIDCR NIH HHS
فهرسة مساهمة:	Keywords: Devils Hole pupfish; inbreeding; mutation load; runs of homozygosity
تواريخ الأحداث:	Date Created: 20221102 Date Completed: 20221103 Latest Revision: 20230526
رمز التحديث:	20230526
مُعرف محوري في PubMed:	PMC9627712
DOI:	10.1098/rspb.2022.1561
PMID:	36321496
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1471-2954
DOI:	10.1098/rspb.2022.1561