دورية أكاديمية
Real world clinical feasibility of direct-from-specimen antimicrobial susceptibility testing of clinical specimens with unknown microbial load or susceptibility.
| العنوان: | Real world clinical feasibility of direct-from-specimen antimicrobial susceptibility testing of clinical specimens with unknown microbial load or susceptibility. |
|---|---|
| المؤلفون: | Chen J; GeneFluidics, Los Angeles, CA, USA., Navarro E; GeneFluidics, Los Angeles, CA, USA., Mesich B; The Medical College of Wisconsin, Milwaukee, WI, USA., Gerstbrein D; The Medical College of Wisconsin, Milwaukee, WI, USA., Cruz A; The Medical College of Wisconsin, Milwaukee, WI, USA., Faron ML; The Medical College of Wisconsin, Milwaukee, WI, USA., Gau V; GeneFluidics, Los Angeles, CA, USA. vgau@genefluidics.com. |
| المصدر: | Scientific reports [Sci Rep] 2022 Nov 02; Vol. 12 (1), pp. 18525. Date of Electronic Publication: 2022 Nov 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/therapeutic use , Ciprofloxacin*/pharmacology , Ciprofloxacin*/therapeutic use, Humans ; Feasibility Studies ; Microbial Sensitivity Tests ; Ampicillin |
| مستخلص: | Within healthcare settings, physicians use antibiograms, which offer information on local susceptibility rates, as an aid in selecting empirical antibiotic therapy and avoiding the prescription of potentially ineffective drugs. While antibiograms display susceptibility and resistance data at hospital, city, or region-specific levels and ultimately enable the initiation of antibiogram-based empirical antibiotic treatment, AST reports at the individual patient level and guides treatments away from broad-spectrum antibiotics towards narrower-spectrum antibiotics or the removal of antibiotics entirely. Despite these advantages, AST traditionally requires a 48- to 72-h turn-around; this window of time can be critical for some antimicrobial therapeutic interventions. Herein, we present a direct-from-specimen AST to reduce the time between patient sampling and receipt of lab AST results. The biggest challenge of performing AST directly from unprocessed clinical specimens with an unknown microbial load is aligning the categorical susceptibility report with CLSI reference methods, which start from a fixed inoculum of 0.5 McFarland units prepared using colonies from a sub-culture. In this pilot clinical feasibility study using de-identified remnant specimens collected from MCW, we observed the high and low ends of microbial loads, demonstrating a final categorical agreement of 87.5% for ampicillin, 100% for ciprofloxacin, and 100% for sulfamethoxazole-trimethoprim. (© 2022. The Author(s).) |
| References: | Arh Hig Rada Toksikol. 2020 Dec 31;71(4):300-311. (PMID: 33410777) PeerJ. 2019 Oct 4;7:e7702. (PMID: 31592346) Sci Rep. 2021 Aug 9;11(1):16069. (PMID: 34373552) Cancer Treat Res. 2019;178:265-283. (PMID: 31209850) Sci Rep. 2017 Apr 25;7(1):1148. (PMID: 28442767) J Clin Microbiol. 2018 Mar 26;56(4):. (PMID: 29367292) Clin Microbiol Infect. 2019 Nov;25(11):1347-1355. (PMID: 31055166) Front Bioeng Biotechnol. 2021 Sep 16;9:744198. (PMID: 34604191) PLoS One. 2022 Feb 16;17(2):e0263868. (PMID: 35171945) MethodsX. 2021 Jul 12;8:101453. (PMID: 34434862) Front Digit Health. 2021 Jun 25;3:645232. (PMID: 34713115) J Urol. 2011 Jan;185(1):148-53. (PMID: 21074208) |
| معلومات مُعتمدة: | R01 AI117059 United States AI NIAID NIH HHS; R44 HD084033 United States HD NICHD NIH HHS |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 5E8K9I0O4U (Ciprofloxacin) 7C782967RD (Ampicillin) |
| تواريخ الأحداث: | Date Created: 20221103 Date Completed: 20221104 Latest Revision: 20230104 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9630444 |
| DOI: | 10.1038/s41598-022-21970-2 |
| PMID: | 36323751 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
|---|---|
| DOI: | 10.1038/s41598-022-21970-2 |