دورية أكاديمية
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Histone H2B.8 compacts flowering plant sperm through chromatin phase separation.

التفاصيل البيبلوغرافية
العنوان: Histone H2B.8 compacts flowering plant sperm through chromatin phase separation.
المؤلفون: Buttress T; Cell and Developmental Biology Department, John Innes Centre, Norwich, UK., He S; Cell and Developmental Biology Department, John Innes Centre, Norwich, UK., Wang L; Beijing Frontier Research Center for Biological Structure, Beijing Advanced Innovation Center for Structural Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.; Institute of Biophysics, Chinese Academy of Science, Beijing, China., Zhou S; Cell and Developmental Biology Department, John Innes Centre, Norwich, UK., Saalbach G; Cell and Developmental Biology Department, John Innes Centre, Norwich, UK., Vickers M; Cell and Developmental Biology Department, John Innes Centre, Norwich, UK., Li G; Institute of Biophysics, Chinese Academy of Science, Beijing, China., Li P; Beijing Frontier Research Center for Biological Structure, Beijing Advanced Innovation Center for Structural Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China. pilongli@mail.tsinghua.edu.cn., Feng X; Cell and Developmental Biology Department, John Innes Centre, Norwich, UK. xiaoqi.feng@jic.ac.uk.
المصدر: Nature [Nature] 2022 Nov; Vol. 611 (7936), pp. 614-622. Date of Electronic Publication: 2022 Nov 02.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
أسماء مطبوعة: Publication: Basingstoke : Nature Publishing Group
Original Publication: London, Macmillan Journals ltd.
مواضيع طبية MeSH: Arabidopsis*/cytology , Arabidopsis*/genetics , Arabidopsis*/metabolism , Chromatin*/chemistry , Chromatin*/genetics , Chromatin*/metabolism , Histones*/classification , Histones*/genetics , Histones*/metabolism , Pollen*/cytology , Pollen*/genetics , Pollen*/metabolism , Cell Size*, Protamines ; Gene Expression Regulation, Plant ; AT Rich Sequence ; Cell Nucleus/genetics ; Mutation ; Cell Nucleus Size ; Phase Transition ; Transcription, Genetic
مستخلص: Sperm chromatin is typically transformed by protamines into a compact and transcriptionally inactive state 1,2 . Sperm cells of flowering plants lack protamines, yet they have small, transcriptionally active nuclei with chromatin condensed through an unknown mechanism 3,4 . Here we show that a histone variant, H2B.8, mediates sperm chromatin and nuclear condensation in Arabidopsis thaliana. Loss of H2B.8 causes enlarged sperm nuclei with dispersed chromatin, whereas ectopic expression in somatic cells produces smaller nuclei with aggregated chromatin. This result demonstrates that H2B.8 is sufficient for chromatin condensation. H2B.8 aggregates transcriptionally inactive AT-rich chromatin into phase-separated condensates, which facilitates nuclear compaction without reducing transcription. Reciprocal crosses show that mutation of h2b.8 reduces male transmission, which suggests that H2B.8-mediated sperm compaction is important for fertility. Altogether, our results reveal a new mechanism of nuclear compaction through global aggregation of unexpressed chromatin. We propose that H2B.8 is an evolutionary innovation of flowering plants that achieves nuclear condensation compatible with active transcription.
(© 2022. The Author(s).)
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معلومات مُعتمدة: BBS/E/J/000PR9773 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
المشرفين على المادة: 0 (Chromatin)
0 (Histones)
0 (Protamines)
تواريخ الأحداث: Date Created: 20221103 Date Completed: 20221129 Latest Revision: 20230315
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9668745
DOI: 10.1038/s41586-022-05386-6
PMID: 36323776
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-4687
DOI:10.1038/s41586-022-05386-6