دورية أكاديمية
أعرض في EDS

Prevalence of Plasmodium falciparum haplotypes associated with resistance to sulfadoxine-pyrimethamine and amodiaquine before and after upscaling of seasonal malaria chemoprevention in seven African countries: a genomic surveillance study.

التفاصيل البيبلوغرافية
العنوان: Prevalence of Plasmodium falciparum haplotypes associated with resistance to sulfadoxine-pyrimethamine and amodiaquine before and after upscaling of seasonal malaria chemoprevention in seven African countries: a genomic surveillance study.
المؤلفون: Beshir KB; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK., Muwanguzi J; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK., Nader J; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK; Department of Genetics and Bioinformatics, Division of Health Data and Digitalization, Norwegian Institute of Public Health, Oslo, Norway., Mansukhani R; Faculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, UK., Traore A; Malaria Research and Training Centre, University of Bamako, Bamako, Mali., Gamougam K; Centre de Support en Santé Internationale, N'Djamena, Chad., Ceesay S; Medical Research Council Laboratories, London School of Hygiene & Tropical Medicine, Fajara, The Gambia., Bazie T; Institute of Health Science Research, Bobo-Dioulasso, Burkina Faso., Kolie F; Université Gamal Abdel Nasser de Conakry, Conakry, Guinea., Lamine MM; Centre de Recherche Médecale et Sanitaire, Niamey, Niger., Cairns M; Faculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, UK., Snell P; Faculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, UK., Scott S; Faculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, UK., Diallo A; Université Cheikh Anta Diop, Dakar, Senegal., Merle CS; Special Programme for Research & Training in Tropical Diseases, WHO, Geneva, Switzerland., NDiaye JL; Université Cheikh Anta Diop, Dakar, Senegal., Razafindralambo L; Catholic Relief Services, Dakar, Senegal., Moroso D; Malaria Consortium, Kampala, Uganda; UK Foreign, Commonwealth, & Development Office, Lagos, Nigeria., Ouedraogo JB; Institute of Health Science Research, Bobo-Dioulasso, Burkina Faso., Zongo I; Institute of Health Science Research, Bobo-Dioulasso, Burkina Faso., Kessely H; Centre de Support en Santé Internationale, N'Djamena, Chad., Doumagoum D; Centre de Support en Santé Internationale, N'Djamena, Chad., Bojang K; Medical Research Council Laboratories, London School of Hygiene & Tropical Medicine, Fajara, The Gambia., Ceesay S; Medical Research Council Laboratories, London School of Hygiene & Tropical Medicine, Fajara, The Gambia., Loua K; Université Gamal Abdel Nasser de Conakry, Conakry, Guinea., Maiga H; Malaria Research and Training Centre, University of Bamako, Bamako, Mali., Dicko A; Malaria Research and Training Centre, University of Bamako, Bamako, Mali., Sagara I; Malaria Research and Training Centre, University of Bamako, Bamako, Mali., Laminou IM; Centre de Recherche Médecale et Sanitaire, Niamey, Niger., Ogboi SJ; Jedima International Health Consult, Lagos, Nigeria., Eloike T; Jedima International Health Consult, Lagos, Nigeria., Milligan P; Faculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, UK., Sutherland CJ; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK. Electronic address: colin.sutherland@lshtm.ac.uk.
المصدر: The Lancet. Infectious diseases [Lancet Infect Dis] 2023 Mar; Vol. 23 (3), pp. 361-370. Date of Electronic Publication: 2022 Oct 31.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: United States NLM ID: 101130150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1474-4457 (Electronic) Linking ISSN: 14733099 NLM ISO Abbreviation: Lancet Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Elsevier Science ; The Lancet Pub. Group, 2001-
مواضيع طبية MeSH: Antimalarials*/therapeutic use , Malaria*/drug therapy , Malaria, Falciparum*/drug therapy, Child ; Humans ; Plasmodium falciparum ; Amodiaquine/therapeutic use ; Haplotypes ; Seasons ; Prevalence ; Pyrimethamine/therapeutic use ; Sulfadoxine/therapeutic use ; Drug Combinations ; Chemoprevention ; Nigeria ; Tetrahydrofolate Dehydrogenase/genetics ; Tetrahydrofolate Dehydrogenase/therapeutic use ; Genomics ; Drug Resistance/genetics
مستخلص: Background: Seasonal malaria chemoprevention is used in 13 countries in the Sahel region of Africa to prevent malaria in children younger than 5 years. Resistance of Plasmodium falciparum to seasonal malaria chemoprevention drugs across the region is a potential threat to this intervention.
Methods: Between December, 2015, and March, 2016, and between December, 2017, and March, 2018, immediately following the 2015 and 2017 malaria transmission seasons, community surveys were done among children younger than 5 years and individuals aged 10-30 years in districts implementing seasonal malaria chemoprevention with sulfadoxine-pyrimethamine and amodiaquine in Burkina Faso, Chad, Guinea, Mali, Nigeria, Niger and The Gambia. Dried blood samples were collected and tested for P falciparum DNA by PCR. Resistance-associated haplotypes of the P falciparum genes crt, mdr1, dhfr, and dhps were identified by quantitative PCR and sequencing of isolates from the collected samples, and survey-weighted prevalence and prevalence ratio between the first and second surveys were estimated for each variant.
Findings: 5130 (17·5%) of 29 274 samples from 2016 and 2176 (7·6%) of 28 546 samples from 2018 were positive for P falciparum on quantitative PCR. Among children younger than 5 years, parasite carriage decreased from 2844 of 14 345 samples (19·8% [95% CI 19·2-20·5]) in 2016 to 801 of 14 019 samples (5·7% [5·3-6·1]) in 2018 (prevalence ratio 0·27 [95% CI 0·24-0·31], p<0·0001). Genotyping found no consistent evidence of increasing prevalence of amodiaquine resistance-associated variants of crt and mdr1 between 2016 and 2018. The dhfr haplotype IRN (consisting of 51Ile-59Arg-108Asn) was common at both survey timepoints, but the dhps haplotype ISGEAA (431Ile-436Ser-437Gly-540Glu-581Ala-613Ala), crucial for resistance to sulfadoxine-pyrimethamine, was always rare. Parasites carrying amodiaquine resistance-associated variants of both crt and mdr1 together with dhfr IRN and dhps ISGEAA occurred in 0·05% of isolates. The emerging dhps haplotype VAGKGS (431Val-436Ala-437Gly-540Lys-581Gly-613Ser) was present in four countries.
Interpretation: In seven African countries, evidence of a significant reduction in parasite carriage among children receiving seasonal malaria chemoprevention was found 2 years after intervention scale-up. Combined resistance-associated haplotypes remained rare, and seasonal malaria chemoprevention with sulfadoxine-pyrimethamine and amodiaquine is expected to retain effectiveness. The threat of future erosion of effectiveness due to dhps variant haplotypes requires further monitoring.
Funding: Unitaid.
Competing Interests: Declaration of interests We declare no competing interests.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved.)
التعليقات: Comment in: Lancet Infect Dis. 2023 Mar;23(3):269-270. (PMID: 36327998)
معلومات مُعتمدة: 001 International WHO_ World Health Organization; MR/T016124/1 United Kingdom MRC_ Medical Research Council
المشرفين على المادة: 37338-39-9 (fanasil, pyrimethamine drug combination)
220236ED28 (Amodiaquine)
0 (Antimalarials)
Z3614QOX8W (Pyrimethamine)
88463U4SM5 (Sulfadoxine)
0 (Drug Combinations)
EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
تواريخ الأحداث: Date Created: 20221103 Date Completed: 20230228 Latest Revision: 20230315
رمز التحديث: 20230315
DOI: 10.1016/S1473-3099(22)00593-X
PMID: 36328000
قاعدة البيانات: MEDLINE
الوصف
تدمد:1474-4457
DOI:10.1016/S1473-3099(22)00593-X